Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro

Gaggi, Giulia; Credico, Andrea Di; Guarnieri, Simone; Mariggiò, Maria Addolorata; Ballerini, Patrizia; Baldassarre, Angela Di; Ghinassi, Barbara

doi:10.1016/j.isci.2022.105197

Cited by 7 publications

(3 citation statements)

References 77 publications

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“…For all the examined mRNAs, qPCR analysis was performed using SYBR green (PowerUp SYBR Green Master mix; Thermo Fisher Scientific) in Quant Studio 3 (Thermo Fisher Scientific) as previously reported. 30 Briefly, the samples were analyzed in triplicate in MicroAmp optical 96-well reaction plates (Thermo Fisher Scientific) and 18S was used as a housekeeping gene. The samples were run as follows: step 1, 95C for 10 min; step 2, 95C for 15 sec; and step 3, 60C for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes

Credico

Gaggi

Izzicupo

et al. 2023

J Histochem Cytochem.

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Skeletal muscle atrophy is represented by a dramatic decrease in muscle mass, and it is related to a lower life expectancy. Among the different causes, chronic inflammation and cancer promote protein loss through the effect of inflammatory cytokines, leading to muscle shrinkage. Thus, the availability of safe methods to counteract inflammation-derived atrophy is of high interest. Betaine is a methyl derivate of glycine and it is an important methyl group donor in transmethylation. Recently, some studies found that betaine could promote muscle growth, and it is also involved in anti-inflammatory mechanisms. Our hypothesis was that betaine would be able to prevent tumor necrosis factor-α (TNF-α)-mediated muscle atrophy in vitro. We treated differentiated C2C12 myotubes for 72 hr with either TNF-α, betaine, or a combination of them. After the treatment, we analyzed total protein synthesis, gene expression, and myotube morphology. Betaine treatment blunted the decrease in muscle protein synthesis rate exerted by TNF-α, and upregulated Mhy1 gene expression in both control and myotube treated with TNF-α. In addition, morphological analysis revealed that myotubes treated with both betaine and TNF-α did not show morphological features of TNF-α-mediated atrophy. We demonstrated that in vitro betaine supplementation counteracts the muscle atrophy led by inflammatory cytokines.

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Section: Methodsmentioning

confidence: 99%

Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes

Credico

Gaggi

Izzicupo

et al. 2023

J Histochem Cytochem.

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“…hFM-MSCs were isolated from the term placentas of 3 healthy donor women as previously described [51]. Cells were cultured in DMEM 10% FBS, 1% penicillin/streptomycin, and 2 mM l-glutamine.…”

Section: Human Cell Cultures and Treatmentsmentioning

confidence: 99%

The Effects of Combined Exposure to Bisphenols and Perfluoroalkyls on Human Perinatal Stem Cells and the Potential Implications for Health Outcomes

Di Credico,

Gaggi,

Bucci

et al. 2023

IJMS

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The present study investigates the impact of two endocrine disruptors, namely Bisphenols (BPs) and Perfluoroalkyls (PFs), on human stem cells. These chemicals leach from plastic, and when ingested through contaminated food and water, they interfere with endogenous hormone signaling, causing various diseases. While the ability of BPs and PFs to cross the placental barrier and accumulate in fetal serum has been documented, the exact consequences for human development require further elucidation. The present research work explored the effects of combined exposure to BPs (BPA or BPS) and PFs (PFOS and PFOA) on human placenta (fetal membrane mesenchymal stromal cells, hFM-MSCs) and amniotic fluid (hAFSCs)-derived stem cells. The effects of the xenobiotics were assessed by analyzing cell proliferation, mitochondrial functionality, and the expression of genes involved in pluripotency and epigenetic regulation, which are crucial for early human development. Our findings demonstrate that antenatal exposure to BPs and/or PFs may alter the biological characteristics of perinatal stem cells and fetal epigenome, with potential implications for health outcomes at birth and in adulthood. Further research is necessary to comprehend the full extent of these effects and their long-term consequences.

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“…Neurodegenerative movement disorders (NMDs) are marked by neuronal degeneration, often accompanied by abnormal protein aggregate buildup in the brain, leading to motor symptoms and cognitive decline [ 1 , 2 , 3 , 4 , 5 ]. This category encompasses various diseases, including Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), and multiple sclerosis (MS), among others.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Epigenetic Landscape: Insights into Parkinson’s Disease, Amyotrophic Lateral Sclerosis, and Multiple Sclerosis

Di Martino,

Marcozzi,

Bibbò

et al. 2024

Brain Sciences

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Parkinson’s disease (PD), multiple sclerosis (MS), and amyotrophic lateral sclerosis (ALS) are examples of neurodegenerative movement disorders (NMDs), which are defined by a gradual loss of motor function that is frequently accompanied by cognitive decline. Although genetic abnormalities have long been acknowledged as significant factors, new research indicates that epigenetic alterations are crucial for the initiation and development of disease. This review delves into the complex interactions that exist between the pathophysiology of NMDs and epigenetic mechanisms such DNA methylation, histone modifications, and non-coding RNAs. Here, we examine how these epigenetic changes could affect protein aggregation, neuroinflammation, and gene expression patterns, thereby influencing the viability and functionality of neurons. Through the clarification of the epigenetic terrain underpinning neurodegenerative movement disorders, this review seeks to enhance comprehension of the underlying mechanisms of the illness and augment the creation of innovative therapeutic strategies.

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Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro

Cited by 7 publications

References 77 publications

Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes

Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes

The Effects of Combined Exposure to Bisphenols and Perfluoroalkyls on Human Perinatal Stem Cells and the Potential Implications for Health Outcomes

Unraveling the Epigenetic Landscape: Insights into Parkinson’s Disease, Amyotrophic Lateral Sclerosis, and Multiple Sclerosis

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