Human fetal mesoangioblasts reveal tissue-dependent transcriptional signatures

Ronzoni, Flavio Lorenzo; Lemeille, Sylvain; Kuzyakiv, Rostyslav; Sampaolesi, Maurilio; Jaconi, Marisa

doi:10.1002/sctm.19-0209

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…In line with Dominici et al [ 12 ], immunophenotypic characterization studies highlighted the negativity to hematopoietic markers (i.e., CD14 and CD45), as well as the positivity for stem cell markers such as CD73, CD105 and CD90. Similar to multipotent cells isolated from the perivascular niche [ 24 ], they were positive for CD34, as well as for markers typically expressed by pericytes [ 25 ], such as CD13 and NG2. Furthermore, the absence of MHC class II molecules (HLA-DR) expression suggests that CMCs transplantation may be connected to a low risk of immune response.…”

Section: Discussionmentioning

confidence: 99%

Growth and Differentiation of Circulating Stem Cells After Extensive Ex Vivo Expansion

Barbon

Rajendran

Bertalot

et al. 2021

Tissue Eng Regen Med

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Background: Stem cell therapy is gaining momentum as an effective treatment strategy for degenerative diseases. Adult stem cells isolated from various sources (i.e., cord blood, bone marrow, adipose tissue) are being considered as a realistic option due to their well-documented therapeutic potentials. Our previous studies standardized a method to isolate circulating multipotent cells (CMCs) that are able to sustain long term in vitro culture and differentiate towards mesodermal lineages. Methods: In this work, long-term cultures of CMCs were stimulated to study in vitro neuronal and myogenic differentiation. After induction, cells were analysed at different time points. Morphological studies were performed by scanning electron microscopy and specific neuronal and myogenic marker expression were evaluated using RT-PCR, flow cytometry and western blot. For myogenic plasticity study, CMCs were transplanted into in vivo model of chemically-induced muscle damage. Results: After neurogenic induction, CMCs showed characteristic dendrite-like morphology and expressed specific neuronal markers both at mRNA and protein level. The calcium flux activity of CMCs under stimulation with potassium chloride and the secretion of noradrenalin confirmed their ability to acquire a functional phenotype. In parallel, the myogenic potential of CMCs was confirmed by their ability to form syncytium-like structures in vitro and express myogenic markers both at early and late phases of differentiation. Interestingly, in a rat model of bupivacaine-induced muscle damage, CMCs integrated within the host tissue taking part in tissue repair. Conclusion: Overall, collected data demonstrated long-term cultured CMCs retain proliferative and differentiative potentials suggesting to be a good candidate for cell therapy.

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Section: Discussionmentioning

confidence: 99%

Growth and Differentiation of Circulating Stem Cells After Extensive Ex Vivo Expansion

Barbon

Rajendran

Bertalot

et al. 2021

Tissue Eng Regen Med

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“…In muscle degenerative disease animal models, several studies have shown the intrinsic capacity of MABs to contribute to muscle regeneration (14)(15)(16)(17)(18). Recently it has been demonstrated that MABs derived from fetal tissues show high plasticity and elevate differentiation capabilities (19). In particular, transcriptional profiles of MABs derived from aorta, atrial, ventricular, and skeletal muscles of fetuses revealed that each subset of MABs displayed a set of differentially expressed genes, which seem to reflect their distinct tissue derivations.…”

Section: Introductionmentioning

confidence: 99%

State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts

Giarratana¹,

Conti²,

Rinvenuti³

et al. 2023

Methods in Molecular Biology

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Adult skeletal muscle is a dynamic tissue able to regenerate quite efficiently thanks to the presence of a stem cell machinery. Besides the quiescent satellite cells that are activated upon injury or paracrine factors other stem cells are described to be directly or indirectly involved in adult myogenesis. Mesoangioblasts (MABs) are vessel-associated stem cells originally isolated form embryonic dorsal aorta and then from adult muscle interstitia expressing pericyte markers.Recently, adult MABs entered clinical trials for the treatment of Duchenne muscular dystrophy and the transcriptome of human fetal MABs has been described. In addition, single cell RNA-seq analyses provide novel information on adult murine MABs and more in general in interstitial muscle stem cells. This chapter provides the state-of-the-art techniques to isolate and characterize murine MABs, fetal and adult human MABs.

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“…However, when muscle loss becomes irreversible (e.g., in case of severe trauma, invasive surgeries, degenerative diseases, or because of aging), lesions are so critical that they impair muscle functionality (Young, 1964). In this scenario, muscle regenerative medicine can provide solutions (Langridge et al., 2021; Ronzoni et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Myoblast 3D bioprinting to burst in vitro skeletal muscle differentiation

Ronzoni

Aliberti

Scocozza

et al. 2022

J Tissue Eng Regen Med

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Skeletal muscle regeneration is one of the major areas of interest in sport medicine as well as trauma centers. Three-dimensional (3D) bioprinting (BioP) is nowadays widely adopted to manufacture 3D constructs for regenerative medicine but a comparison between the available biomaterial-based inks (bioinks) is missing. The present study aims to assess the impact of different hydrogels on the viability, proliferation, and differentiation of murine myoblasts (C2C12) encapsulated in 3D bioprinted constructs aided to muscle regeneration. We tested three different commercially available hydrogels bioinks based on: (1) gelatin methacrylate and alginate crosslinked by UV light; (2) gelatin methacrylate, xanthan gum, and alginate-fibrinogen; (3) nanofibrillated cellulose (NFC)/alginate-fibrinogen crosslinked with calcium chloride and thrombin. Constructs embedding the cells were manufactured by extrusion-based BioP and C2C12 viability, proliferation, and differentiation were assessed after 24 h, 7, 14, 21, and 28 days in culture. Although viability, proliferation, and differentiation were observed in all the constructs, among the investigated bioinks, the best results were obtained by using NFC/alginatefibrinogen-based hydrogel from 7 to 14 days in culture, when the embedded myoblasts started fusing, forming at day 21 and day 28 multinucleated myotubes within the 3D bioprinted structures. The results revealed an extensive myotube alignment all over the linear structure of the hydrogel, demonstrating cell maturation, and enhanced myogenesis. The bioprinting strategies that we describe here denote a strong and endorsed approach for the creation of in vitro artificial muscle to improve skeletal muscle tissue engineering for future therapeutic applications.

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Human fetal mesoangioblasts reveal tissue-dependent transcriptional signatures

Cited by 5 publications

References 36 publications

Growth and Differentiation of Circulating Stem Cells After Extensive Ex Vivo Expansion

Growth and Differentiation of Circulating Stem Cells After Extensive Ex Vivo Expansion

State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts

Myoblast 3D bioprinting to burst in vitro skeletal muscle differentiation

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