Human follicular fluid and mouse cumulus cells act synergistically to enhance preimplantation mouse Balb/cJ embryo development

Roudebush, William E.; Levine, Adam Seth; Lodge, Jeffrey S.; Tsai, Chun-Chin; Butler, Warren L.

doi:10.1007/bf02212902

Cited by 3 publications

(4 citation statements)

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“…In our study neat heat inactivated FF was used and the results indicate that the fluid and mouse cumulus cells not only improved the culture conditions independently but also synergistically enhanced the mouse embryo development. This finding confirms the report of Roudebush et al (1995) however, they failed to find any positive effects on embryo development when follicular fluid and cumulus cells were used independently [25]. Follicular fluid contains all the basic materials required for in vitro embryo growth [26].…”

Section: Discussionsupporting

confidence: 85%

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Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Malekshah

Moghaddam

2005

Journal of Reproduction and Development

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Abstract. The development of preimplantation mammalian embryos in vitro is less than optimal. Follicular fluid and cumulus cells have both been used independently, to improve preimplantation embryo quality in culture. This study was undertaken to evaluate the influence of a cumulus cell monolayer in human follicular fluid on mouse early embryo development in vitro. One-cell embryos were obtained from NMRI mice after superovulation with eCG and hCG. Cumulus cells were prepared from mouse egg-cumulus mass. These cells were separated from red blood cells using a Percoll gradient. Follicular fluid was collected from patients undergoing an IVF program during oocyte pick-up. The cumulus cell monolayer was prepared in follicular fluid (FC) and Ham's F10 (HC). Mouse one-cell embryos were cultured in FC, HC, Ham's F10 (HF) and follicular fluid (FF) for 120 h. Only 10.5% of embryos passed the two-cell block in HF. However, the proportions of embryos passing the two-cell block were 23.1%, 21.4% and 68.5% in FF, HC, and FC treatments, respectively; which were significantly different from HF (p<0.05). The differences between FC and the two other treatments were also significant (p<0.001). In FC, 33.7% of one-cell embryos continued to grow to the blastocyst stage whereas only 2.1% and 1.9% of one-cell embryos in FF and HC reached this stage and no embryos developed to blastocyst in HF. The proportion of blastocysts in FC was significantly higher than all other treatments (p<0.001). It can be concluded that follicular fluid and cumulus cells in monolayer form synergistically improve the early embryo culture condition.

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Section: Discussionsupporting

confidence: 85%

“…Hemmings et al (1994) showed the beneficial effects of FF on the in vitro development of human pre-embryos [13]. However, some other studies did not confirm these effects of FF [23][24][25]. This controversy may be due to the fact that the origin of follicular fluid and its proportion used in the media were different.…”

Section: Discussionmentioning

confidence: 99%

Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Malekshah

Moghaddam

2005

Journal of Reproduction and Development

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“…Benkhalifa et al reported similar findings but also demonstrated that the cumulus was producing embryotrophic factors such as leukemia inhibitor factor and platelet activating factor receptor (114). These studies are a few of the many studies that have reported a benefit of coculture with cumulus cells, including studies in other species (115). Interestingly, these studies include a ''sequential medium'' cumulus coculture in mice demonstrating greatly improved blastulation rates when sequential media were used in conjunction with coculture, compared with sequential media alone (116).…”

Section: Clinical Studies Of Coculture Systemsmentioning

confidence: 69%

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential

Swain¹,

Carrell

Cobo

et al. 2016

Fertility and Sterility

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With increased use of comprehensive chromosome screening (CCS), the question remains as to why some practices do not experience the same high levels of clinical success after implementation of the approach. Indeed, the debate surrounding the efficacy and usefulness of blastocyst biopsy and CCS continues. Importantly, several variables impact the success of an assisted reproductive technology cycle. Transfer of a euploid embryo is but one factor in an intricate system that requires numerous steps to occur successfully. Certainly, the culture environment and the manipulations of the embryo during its time in the laboratory can impact its reproductive potential. Environmental stressors ranging from culture media to culture conditions and even culture platform can impact biochemical, metabolic, and epigenetic patterns that can affect the developing cell independent of chromosome number. Furthermore, accompanying procedures, such as biopsy and vitrification, are complex and, when performed improperly, can negatively impact embryo quality. These are areas that likely still carry room for improvement within the IVF laboratory.

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“…Dirnfeld et al recently demonstrated that a coculture system using autologous granulosa cells recovered during oocyte aspiration was beneficial in improving embryo quality and pregnancy rates in patients with a prior cycle of poor embryo development (12). Additionally, cumulus cells have been shown to improve blastocyst development in both the human and the mouse (13,14).…”

Section: Introductionmentioning

confidence: 99%

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Carrell

Peterson

Jones

et al. 1999

Journal of Assisted Reproduction and Genetics

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Human follicular fluid and mouse cumulus cells act synergistically to enhance preimplantation mouse Balb/cJ embryo development

Abstract: Cumulus cells and follicular fluid have a positive synergistic affect on preimplantation mouse Balb/cJ embryo development and formation in vitro.

Cited by 3 publications

References 20 publications

Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential

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