Human gait data becomes very helpful in the prediction and correction of movement control while walking. In many areas like medical practices, Physical fitness training programs, rehabilitation techniques and prosthesis performance, gait study has its significant scientific relevance and applications. This study aims to predict the performance of Jaipur knee joint in terms of gait symmetry with transfemoral Indian amputees. First time we tried to calculate gait symmetry of widely used Jaipur knee joint with Indian population. This aspect has not been focused so far with Indian amputees. 11 unilateral transfemoral amputees (9 men and 2 women; average age 45 years, range 31-58 years) participated in the study. Subjects were using prosthesis with Jaipur knee for long time (mean: 16 yrs; range: 5-27 yrs). To investigate the gait function with 6 Vicon cameras 3D motion analysis system (Kinematrix system) participants were asked to walk with their comfortable speed. Gait symmetry, might be the basis of recommendation of knee joint. Kinematics parameter is predicted quantitavely gait symmetry with Jaipur knee joint. This research will result in significant prevention of degenerated musculoskeletal effects in future generally seen in unilateral transfemoral amputees.