Human gene MOB: structure specification and aspects of transcriptional activity

Vladychenskaya, Irina P.; Dergunova, Lyudmila V.; Dmitrieva, Veronica G.; Limborska, Svetlana A.

doi:10.1016/j.gene.2004.06.003

Cited by 17 publications

(12 citation statements)

References 13 publications

(19 reference statements)

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“…60 Structural and functional analysis of its transcripts predicted regulation at both transcriptional and translational level. 60,61 Subsequently, several alternative spliced products of SMS1 were found both in human cerebellum and in the mouse 62 suggesting complex regulation for SMS1 expression.…”

Section: Sms Cloning Strategiesmentioning

confidence: 99%

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Holthuis

Luberto

2010

Advances in Experimental Medicine and Biology

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In the last five years tremendous progress has been made toward the understanding of the mechanisms that govern sphingomyelin (SM) synthesis in animal cells. In line with the complexity of most biological processes, also in the case of SM biosynthesis, the more we learn the more enigmatic and finely tuned the system appears. Therefore with this review we aim first, at highlighting the most significant discoveries that advanced our knowledge and understanding of SM biosynthesis, starting from the discovery of SM to the identification of the enzymes responsible for its production; and second, at discussing old and new riddles that such discoveries pose to current investigators.

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Section: Sms Cloning Strategiesmentioning

confidence: 99%

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Holthuis

Luberto

2010

Advances in Experimental Medicine and Biology

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“…Gene-specific primers were designed using OLIGO Primer Analysis software (Wojciech and Piotr Rychlik copyright, version 6.31) ( Table 1). Aliquots of the PCR products were size-fractionated on silver-stained 6% PAAG as previously described (Vladychenskaya et al 2004). For real-time PCR quantitation, equal amounts of cDNA were added to a set of PCR with mastermix Buffer B SYBR Green I (Syntol, Russia) and primers for glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as the internal control and genes examined.…”

Section: Rt-pcr and Real-time Pcr Quantitationmentioning

confidence: 99%

Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia

Дмитриева

Povarova²,

Skvortsova³

et al. 2009

Cell Mol Neurobiol

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Consisting of a fragment of ACTH(4-7) and C-terminal PGP tripeptide, the polypeptide Semax is successfully used for acute stroke therapy. Previous experiments showed rapid induction of Bdnf, Ngf, and TrkB expression in intact rat hippocampus following Semax treatment. To investigate the mRNA expression of neurotrophins and their receptors after treatment with either Semax or PGP, the rat brains were analyzed at three time points following a permanent middle cerebral artery occlusion (pMCAO). We have shown for the first time that both Semax and PGP activate the transcription of neurotrophins and their receptors in the cortex of rats subjected to pMCAO. The profiles of transcription alteration under PGP and Semax treatment were partially overlapped. Semax enhanced the transcription of Bdnf, TrkC, and TrkA 3 h after occlusion, Nt-3 and Ngf 24 h after occlusion, and Ngf 72 h after occlusion. PGP enhanced the transcription of Bdnf and TrkC 3 h after pMCAO and Ngf, TrkB, TrkC, and TrkA 24 h after pMCAO. The analysis of the transcription alterations under PGP and Semax treatment in the cortex of rats without surgery, sham-operated rats and rats subjected to pMCAO revealed that Semax selectively affected the transcription of neurotrophins and their receptors in the ischemic rat cortex, whereas the influence of PGP was mainly unspecific.

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“…We established that the transcriptional diversity of the SGMS1 gene is provided by the presence of alternative promoters, alternative polyadenylation and exon splicing. [5][6][7][8][9] In human tissues, transcripts with a 5 0 untranslated region (5 0 UTR) that includes exons 1-6 and exon 7, with a coding region that is localized in exons 7-10 and in a portion of exon 11, and with a 3 0 UTR that stems entirely to the remaining portion of exon 11 are mostly abundant. 8 The multiplicity and complexity of the biological processes that involve the sphingomyelin synthase 1 (SMS1) protein suggest the high specificity of the control of the function of its encoding gene in the individual cells and tissues of organisms.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA of the human sphingomyelin synthase 1 gene: Multiple splice variants, evolutionary conservatism and expression in different tissues

et al. 2015

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The human sphingomyelin synthase 1 gene (SGMS1) encodes an essential enzyme that is involved in the synthesis of sphingomyelin and diacylglycerol from phosphatidylcholine and ceramide. Among the products of SGMS1, we found new transcripts, circular RNAs (circRNAs), that contain sequences of the gene's 5 0 untranslated region (5 0 UTR). Some of them include the gene's coding region and fragments of introns. An analysis of the abundance of circRNAs in human tissues showed that the largest transcripts were predominantly found in different parts of the brain. circRNAs of rat and mouse sphingomyelin synthase 1 orthologous genes were detected and are highly similar to the human SGMS1 gene transcripts. A quantitative analysis of the abundance of such transcripts also revealed their elevated amount in the brain. A computational analysis of sequences of human circRNAs showed their high potential of binding microRNAs (miRNAs), including the miRNAs that form complexes with Ago proteins and the mRNA of SGMS1. We assume that the circRNAs identified here participate in the regulation of the function of the SGMS1 gene in the brain.

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Human gene MOB: structure specification and aspects of transcriptional activity

Cited by 17 publications

References 13 publications

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Tales and Mysteries of the Enigmatic Sphingomyelin Synthase Family

Semax and Pro-Gly-Pro Activate the Transcription of Neurotrophins and Their Receptor Genes after Cerebral Ischemia

Circular RNA of the human sphingomyelin synthase 1 gene: Multiple splice variants, evolutionary conservatism and expression in different tissues

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