Human Gingival Fibroblast Integrin Subunit Expression on Titanium Implant Surfaces

Oates, Thomas W.; Maller, Steven C.; West, Jason D.; Steffensen, Bjørn

doi:10.1902/jop.2005.76.10.1743

Cited by 34 publications

(26 citation statements)

References 34 publications

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“…It has been well indicated that integrins, whose α and β subunits constitute non-covalently linked αβ heterodimers, are responsible for cell adhesion [33]. Several integrin subunits including α2, α5, β1 and β3 were identified in the periodontal tissue [18,34]. Given the interplay of integrins with the extracellular matrix (ECM) and cytoskeleton [35], they regulate cellular functions, cell proliferation, adhesion, shape, and differentiation [32].…”

Section: Resultsmentioning

confidence: 99%

“…In vitro observation showed the presence of integrin subunits in human gingival fibroblasts (HGFs) and the morphological alteration of HGFs due to the surface roughness of Ti [18]. Surface topography is an important factor in cell attachment, adhesion, proliferation, and differentiation [19], which also affects cell orientation and migration [20].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

et al. 2015

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It was indicated that tetragonal zirconia polycrystal (TZP) containing yttria (Y2O3) and niobium oxide (Nb2O5) ((Y,Nb)-TZP) could be an adequate dental material to be used at esthetically important sites. The (Y,Nb)-TZP was also proved to possess its osteogenic potential comparable with those conventional dental implant material, titanium (Ti). The objective of the current study was to characterize cellular response of human gingival fibroblasts (HGFs) to smooth and rough surfaces of the (Y,Nb)-TZP disc, which were obtained by polishing and sandblasting, respectively. Various microscopic, biochemical, and molecular techniques were used to investigate the disc surfaces and cellular responses for the experimental (Y,Nb)-TZP and the comparing Ti groups. Sandblasted rough (Y,Nb)-TZP (Zir-R) discs had the highest surface roughness. HGFs cultured on polished (Y,Nb)-TZP (Zir) showed a rounded cell morphology and light spreading at 6 h after seeding and its proliferation rate significantly increased during seven days of culture compared to other surfaces. The mRNA expressions of type I collagen, integrin α2 and β1 were significantly stimulated for the Zir group at 24 h after seeding. The current findings, combined with the previous results, indicate that (Y,Nb)-TZP provides appropriate surface condition for osseointegration at the fixture level and for peri-implant mucosal sealing at the abutment level producing a suitable candidate for dental implantation with an expected favorable clinical outcome.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

et al. 2015

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“…In another study, PDL fibroblasts display increased spreading on smooth rather than SLA titanium surfaces 37 . Gingival fibroblast attachment to smooth rather than SLA surfaces and smooth titanium surfaces enhanced expression of the ECM receptors, integrin α4 and β1 subunits 38 . PDL cell attachment to all types of titanium surfaces is inhabited by antibodies to the integrin β1 subunit (common to several ECM receptors) and arginine‐glycine‐aspartic acid–containing peptides (the common integrin binding site for fibronectin, fibrinogen, and many other ECM molecules) 39 .…”

Section: Discussionmentioning

confidence: 93%

Altered Cell Motility and Attachment With Titanium Surface Modifications

Palaiologou

Stoute

Fan

et al. 2012

Journal of Periodontology

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“…One established solution is the development of adjusted Ti/Nb/Zr/Sn titanium alloys whose Young’s modulus is close to the physiological Young’s modulus of approximately 50 GPa [91]. Two attractive functionalization procedures have been described, first the immobilization of the surface with RGD-ligands [92] and second, tailoring/patterning the surface of the titanium material with nano-spikes which fit in the architectural arrangement of the integrin receptors of the bone cells by which a functional morphology and an expression of growth factors in bone cells are elicited [93]. …”

Section: Scaffoldmentioning

confidence: 99%

The Deep-Sea Natural Products, Biogenic Polyphosphate (Bio-PolyP) and Biogenic Silica (Bio-Silica), as Biomimetic Scaffolds for Bone Tissue Engineering: Fabrication of a Morphogenetically-Active Polymer

et al. 2013

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Bone defects in human, caused by fractures/nonunions or trauma, gain increasing impact and have become a medical challenge in the present-day aging population. Frequently, those fractures require surgical intervention which ideally relies on autografts or suboptimally on allografts. Therefore, it is pressing and likewise challenging to develop bone substitution materials to heal bone defects. During the differentiation of osteoblasts from their mesenchymal progenitor/stem cells and of osteoclasts from their hemopoietic precursor cells, a lineage-specific release of growth factors and a trans-lineage homeostatic cross-talk via signaling molecules take place. Hence, the major hurdle is to fabricate a template that is functioning in a way mimicking the morphogenetic, inductive role(s) of the native extracellular matrix. In the last few years, two naturally occurring polymers that are produced by deep-sea sponges, the biogenic polyphosphate (bio-polyP) and biogenic silica (bio-silica) have also been identified as promoting morphogenetic on both osteoblasts and osteoclasts. These polymers elicit cytokines that affect bone mineralization (hydroxyapatite formation). In this manner, bio-silica and bio-polyP cause an increased release of BMP-2, the key mediator activating the anabolic arm of the hydroxyapatite forming cells, and of RANKL. In addition, bio-polyP inhibits the progression of the pre-osteoclasts to functionally active osteoclasts. Based on these findings, new bioinspired strategies for the fabrication of bone biomimetic templates have been developed applying 3D-printing techniques. Finally, a strategy is outlined by which these two morphogenetically active polymers might be used to develop a novel functionally active polymer.

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Human Gingival Fibroblast Integrin Subunit Expression on Titanium Implant Surfaces

Cited by 34 publications

References 34 publications

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

Altered Cell Motility and Attachment With Titanium Surface Modifications

The Deep-Sea Natural Products, Biogenic Polyphosphate (Bio-PolyP) and Biogenic Silica (Bio-Silica), as Biomimetic Scaffolds for Bone Tissue Engineering: Fabrication of a Morphogenetically-Active Polymer

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