Human Glioma Cell Lines: Tumour Associated Antigens Distribution and Sensitivity to Antibody-Toxin or Ligand-Toxin Conjugates. A Preliminary Report

Colombatti, Marco; Bisconti, Massimo; Lorenzi, Pamela; Stevanoni, Gabriella; Dipasquale, Bruno; Gerosa, Massimo; Tridente, Giuseppe

doi:10.1007/978-3-7091-8978-8_26

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…IFN-α also increased CEA in vitro [ 127 , 129 ] and in carcinoma patients [ 130 ]. These agents are able to upregulate other TAAs, including tumor-associated CA125 antigens in ovarian cancer cells [ 131 ], glycoprotein-72 (TAG-72) in patients with colon [ 128 , 130 ] or breast cancer [ 132 ], and TAAs in glioma [ 133 , 134 ] or breast cancer cell lines [ 135 ]. Moreover, EpCAM, a TAA detectable in colon cancer cells, was also found to be upregulated by IFN-α and IFN-γ [ 136 ].…”

Section: Drug-induced Upregulation Of Tumor Immunogenicitymentioning

confidence: 99%

Abscopal Effect and Drug-Induced Xenogenization: A Strategic Alliance in Cancer Treatment?

Franzese

Torino

Giannetti

et al. 2021

IJMS

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The current state of cancer treatment is still far from being satisfactory considering the strong impairment of patients’ quality of life and the high lethality of malignant diseases. Therefore, it is critical for innovative approaches to be tested in the near future. In view of the crucial role that is played by tumor immunity, the present review provides essential information on the immune-mediated effects potentially generated by the interplay between ionizing radiation and cytotoxic antitumor agents when interacting with target malignant cells. Therefore, the radiation-dependent abscopal effect (i.e., a biological effect of ionizing radiation that occurs outside the irradiated field), the influence of cancer chemotherapy on the antigenic pattern of target neoplastic cells, and the immunogenic cell death (ICD) caused by anticancer agents are the main topics of this presentation. It is widely accepted that tumor immunity plays a fundamental role in generating an abscopal effect and that anticancer drugs can profoundly influence not only the host immune responses, but also the immunogenic pattern of malignant cells. Remarkably, several anticancer drugs impact both the abscopal effect and ICD. In addition, certain classes of anticancer agents are able to amplify already expressed tumor-associated antigens (TAA). More importantly, other drugs, especially triazenes, induce the appearance of new tumor neoantigens (TNA), a phenomenon that we termed drug-induced xenogenization (DIX). The adoption of the abscopal effect is proposed as a potential therapeutic modality when properly applied concomitantly with drug-induced increase in tumor cell immunogenicity and ICD. Although little to no preclinical or clinical studies are presently available on this subject, we discuss this issue in terms of potential mechanisms and therapeutic benefits. Upcoming investigations are aimed at evaluating how chemical anticancer drugs, radiation, and immunotherapies are interacting and cooperate in evoking the abscopal effect, tumor xenogenization and ICD, paving the way for new and possibly successful approaches in cancer therapy.

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Section: Drug-induced Upregulation Of Tumor Immunogenicitymentioning

confidence: 99%

Abscopal Effect and Drug-Induced Xenogenization: A Strategic Alliance in Cancer Treatment?

Franzese

Torino

Giannetti

et al. 2021

IJMS

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“…Two conceptual approaches for targeting this receptor have been designed and studied separately [3,4]. Constructs have been made using the plasma protein transferrin (Tf) as targeting ligand [5–8]. Alternatively, antitransferrin receptor monoclonal antibodies (α‐TfR mAbs) or, more recently, recombinant single chain variable fragments (α‐TfR ScFv) have been used [9–14].…”

Section: Introductionmentioning

confidence: 99%

Transferrin toxin but not transferrin receptor immunotoxin is influenced by free transferrin and iron saturation

Gosselaar

van-Dijk

Degast

et al. 2002

Eur J Clin Investigation

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Untitled

Farr-Jones

Parney

Petruk

1999

Journal of Neuro-Oncology

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Culturing human central nervous system tumors has been difficult compared to other neoplasms. We report improved success rates for establishing short term human brain tumor cultures using a modified tissue processing technique. Eighty-seven brain tumor specimens (56 glioblastomas, 8 mid grade astrocytomas, 8 oligodendrogliomas, 15 other) were obtained from June 1988 to March 1997. The first twenty-three samples were processed by dissection, partial enzyme dissociation, and filtration through a tissue culture sieve. Subsequent samples were processed identically except tumor cells were centrifuged on a density gradient prior to plating. Successful cultures were defined as those surviving greater than three passages in tissue culture and growing to sufficient numbers (>10(6) cells) to allow freezing. Success rate was 42% (10/23) using standard processing methods and 86% (55/64) with the addition of density gradient centrifugation. Glial fibrillary acidic protein (GFAP) and vimentin staining, karyotypes, and growth curves were obtained for representative glioma cultures. All cultures tested were positive for vimentin (29/29) while 62% (18/29) were positive for GFAP. Of four cultures karyotyped (two glioblastomas, two oligodendrogliomas), all but one oligodendroglioma culture exhibited clonal cytogenetic abnormalities. These immunohistochemical and karyotypic results are consistent with the malignant glial origin of these cells. Of note, low passage human glioma cultures grew slower and exhibited more contact inhibition than immortalized human glioblastoma cell lines. Nevertheless, this simple method for establishing short term human brain tumor cultures should aid in further developing human brain tumor pre-clinical models as well as enhancing clinical applications dependent on in vitro human brain tumor cell growth adjust.

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Human Glioma Cell Lines: Tumour Associated Antigens Distribution and Sensitivity to Antibody-Toxin or Ligand-Toxin Conjugates. A Preliminary Report

Cited by 3 publications

References 15 publications

Abscopal Effect and Drug-Induced Xenogenization: A Strategic Alliance in Cancer Treatment?

Abscopal Effect and Drug-Induced Xenogenization: A Strategic Alliance in Cancer Treatment?

Transferrin toxin but not transferrin receptor immunotoxin is influenced by free transferrin and iron saturation

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