Human growth hormone in urine: development of an ultrasensitive radiometric assay

Erb, T; Károlyi, G; Pampalone, A; Eberlé, Alex N.; Baumann, J. M.; Bürgisser, Ernst; Girard, J.

doi:10.1007/bf01952201

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…The details of the method have been published elsewhere [7,16]. In short, an anti-human GH antiserum was raised in goats.…”

Section: Methods and Subjectsmentioning

confidence: 99%

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Measurement of Urinary Growth Hormone

Girard

Fischer-Wasels

1990

Horm Res

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Using pharmacological tests or GH profiles, GH deficiency or active acromegaly can be diagnosed. However, it is impossible to discriminate within the continuum between ‘deficiency/insufficiency’ and ‘sufficient secretion’. The use of GH to improve growth velocity is based on 1 injection once a day. It is thus the total amount of GH which appears to be important for its growth-promoting effect. An assay of GH in urine allows to assess such GH ‘production’, even over a prolonged period of time. A radiometric two-step assay applicable to untreated urine is presented. Results for assessing the ‘GH status’ by measuring GH in 24-hour urine, first morning-void urine or timed urine samples are shown. The correlation between 24-hour plasma profiles and the simultaneously collected 24-hour urine is significant at p < 0.01. A correlation coefficient > 0.9 was found between timed urine samples (4 h) and the 120-min plasma GH values during GHRH stimulation tests. The night-to-night variation of urinary GH can be very important. It is advisable, therefore, to use the mean of several night urines. The correlation between the mean urinary GH of 5 nights to one 24-hour sample is significant at p < 0.01. An age-dependent increase in urinary GH is found in the pubertal age group. The percentile distribution of 24-hour and night urines for the age groups < 7 years, prepuberty and puberty is given (P₅₀ GH in ng/night, mean of 3 urines: < 7 years = 0.49, prepubertal = 0.75, pubertal = 1.3). The respective 24-hour excretions are: < 7 years = 1.59, prepubertal = 2.62, and pubertal = 4.1 ng/24h Testosterone treatment of delayed puberty dramatically increases endogenous GH production, as shown by the urinary output. The high GH dose used to treat girls with Turner’s syndrome is reflected in a urinary output which is very similar to that found in boys on testosterone treatment. No correlation could be found between the GH dose used as supplementary treatment and urinary output. Furthermore, during GH therapy, the intraindividual excretion varies largely. It is concluded that urinary GH reflects the circulating GH, timed samples show the corresponding plasma values, the mean of several night (first morning-void) urines is a useful tool for assessing the GH status. Although pulsatility is obviously not reflected in urine, the varying total amount of GH secreted is modulated by pulse amplitude, and this is reflected indirectly in urinary GH. Urinary GH can be used to monitor GH therapy or any therapy intended to impair or increase endogenous GH production.

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“…The details of the method have been published elsewhere [7,16]. In short, an anti-human GH antiserum was raised in goats.…”

Section: Methods and Subjectsmentioning

confidence: 99%

“…Several groups have set up methods which fulfill some or all of these criteria [8][9][10][11][12][13][14]. In our laboratory, we have developed an immunoradiometric assay which does not require any pretreatment or con centration of urine samples [7,[15][16][17][18].…”

mentioning

confidence: 99%

Measurement of Urinary Growth Hormone

Girard

Fischer-Wasels

1990

Horm Res

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“…To remove the deleterious effects of these urinary constituents and standardize the pH, most methods dialyse the urine sample for 24 hours prior to assay. Other methods use diluted urine (Girard et al, 1987;Erb et al, 1988), ultrafiltrated urine (Pholsena et al, 1993), or the effects of urine are minimized with a high molarity (0.45 M) buffer . Both Erb ef al.…”

Section: Lmmunometric Assaysmentioning

confidence: 99%

Current methods for the measurement of growth hormone in urine

Hourd

Edwards

1994

Clinical Endocrinology

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“…Furthermore, a 24-hour HGH pro duction appears to be reflected in its urinary excretion [13], The very low levels to be expected in urine require, however, an ex tremely sensitive assay for its quantification. It was the purpose of this investigation there fore to develop a sensitive assay for HGH, which can be applied to plasma and urine [14,15] and to report preliminary results.…”

Section: Introductionmentioning

confidence: 99%

Growth Hormone in Urine: Development of an Ultrasensitive Assay Applicable to Plasma and Urine

Girard

Erb²,

Pampalone³

et al. 1987

Horm Res

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A radiometric assay for human growth hormone (HGH) was developed based on a polyclonal goat anti-HGH antiserum covalently coupled to nonsedimenting polyacrylamid particles. HGH can be specifically immunoextracted from sample volumes of up to 10 ml. Subsequently, bound HGH is identified and quantitatively measured by a ¹²⁵I-labelled monoclonal anti-HGH antibody. The assay is insensitive to plasma proteins from 10 to > 90%, to changing NaCl and urea molarities and to pH ranges from 6 to 8. The sensitivity in the second incubation is 2 pg/tube, corresponding to a maximum sensitivity of 300 fg/ml of a sample volume of 10 ml (urine) or of 40 pg/ml, if a volume of 50 µl (plasma) is assayed. In healthy children, a mean HGH excretion of 6.5 ng/24 h was found with a large interindividual range from undetectable to 37.4 ng. An important intraindividual night-tonight variation of HGH excretion was found in several subsequent first morning void samples in healthy children. The mean excretion in 13 HGH-deficient children was 0.9 ng/24 h off therapy and increased to a mean of 6.9 ng/24 h on therapy. In acromegalic patients, the excreted HGH amounted to 73–208 ng/24 h. Preliminary results suggest that the ultrasensitive assay applied to plasma and urine could be a considerable improvement of diagnosis and follow-up of disorders of HGH secretion.

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Human growth hormone in urine: development of an ultrasensitive radiometric assay

Cited by 10 publications

References 18 publications

Measurement of Urinary Growth Hormone

Measurement of Urinary Growth Hormone

Current methods for the measurement of growth hormone in urine

Growth Hormone in Urine: Development of an Ultrasensitive Assay Applicable to Plasma and Urine

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