Human Hair Keratin Composite Scaffold: Characterisation and Biocompatibility Study on NIH 3T3 Fibroblast Cells

Mohamed, Jamal Moideen Muthu; Al-Qahtani, Ali S.; Fatease, Adel Al; Alqahtani, Taha; Khan, Barkat Ali; Ashmitha, B.; Vijaya, R

doi:10.3390/ph14080781

Cited by 32 publications

(18 citation statements)

References 46 publications

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“…Free drug in the supernatant liquid was determined by utilizing the UV-spectrophotometer at 245 nm. Methanol was utilized for the necessary dilution process, and the solution was then filtered [ 11 ]. The drug entrapment and loading was calculated from following formula:

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Section: Methodsmentioning

confidence: 99%

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Development of Atorvastatin Calcium Biloaded Capsules for Oral Administration of Hypercholesterolemia

Senthilvel

Kavitha

Pothumani

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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The goal of this study was to develop atorvastatin (ATN) calcium biloaded, i.e., immediate release (IR) and sustained release (SR) capsules that would promote the quick onset of action and a better dissolution profile on both the IR and SR aspects. The IR granules were prepared by the wet granulation method, and an aqueous solubility study proved that the IR granules released the ATN within 25 min compared to the pure drug due to the addition of PEG and super disintegrants such as croscarmellose (CC) and crospovidone (CP). The sustained release nanoparticles (SR-NPs) were synthesized using a solvent evaporation technique and an optimal combination of hydrophilic and hydrophobic polymers. The addition of a hydrophobic polymer to a hydrophilic polymer delays drug release, resulting in a sustained and controlled release lasting up to 12 hours. The drug release of ATN from SR nanoparticles followed the Higuchi and Korsmeyer–Peppas models and had first-order kinetics (r2 = ???). Fourier transform infrared spectrophotometry, powder X-ray diffraction, and differential scanning calorimetric analysis were used to test the prepared biloaded capsules, and the results showed that there was no significant interaction between the polymers, excipients, and drug. The SEM and DLS analysis clearly demonstrated that drug particles in a continuous layer were enclosed by polymers at the nanoscale. To summarise, integrating both layers into a single capsule resulted in a superior release profile and patient compliance. Finally, prepared biloaded capsules were discovered to exhibit both an IR and an SR profile.

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…”

Section: Methodsmentioning

confidence: 99%

“…In order to investigate the drug release mechanism from nanoparticle formulations, the percentage cumulative drug release data was fitted into mathematical models like first order, Higuchi's model, and the Korsmeyer–Peppas model. The mean results of triplicate measurements and the standard deviation were reported [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Development of Atorvastatin Calcium Biloaded Capsules for Oral Administration of Hypercholesterolemia

Senthilvel

Kavitha

Pothumani

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The average particle size of ITZ-nPEVs varied from 196.55 ± 0.025 to 252.2 ± 0.019 nm, as reported in Table 1 and Figure S2a. The PDI values of ITZ-nPEVs did not exceed 0.4, indicating that the solution was homogeneous and monodisperse [14]. It was observed that the concentration of lipid increases, the particle size of formulated ITZ-nPEVs also increased.…”

Section: Ps Pdi and Zpmentioning

confidence: 95%

The Unique Carboxymethyl Fenugreek Gum Gel Loaded Itraconazole Self-Emulsifying Nanovesicles for Topical Onychomycosis Treatment

et al. 2022

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The novel itraconazole (ITZ) nail penetration enhancing self-emulsifying nanovesicles (ITZ-nPEVs) loaded in carboxymethyl fenugreek gum (CMFG) gel circumvent the systemic onychomycosis treatment. The ITZ-nPEVs were prepared by the thin film hydration technique, and the particle size (PS), zeta potential (ZP), drug content (DC), entrapment efficiency (% EE), deformity index (DI), viscosity, morphology, and physical stability of the ITZ-nPEVs were measured. In terms of nail hydration, transungual drug absorption, and antifungal efficacy against Candida albicans, the chosen ITZ-nPEVs, nPEV-loaded CMFG (CMFG-ITZ-nPEVs) gel, and the commercialized Itrostred gel were compared. The ITZ-nPEVs showed spherical structure with high DC, % EE, low PS and PDI and positive ZP of ITZ ranging from 95.36 to 93.89 mg/5 mL and 95.36–96.94%, 196.55–252.5 nm, 0.092–0.49, and +11.1 to +22.5 mV, respectively. Compared to the Itrostred gel, the novel ITZ-nPEVs exhibited hydration enhancement factor for 24 h (HE24) of 1.53 and 1.39 drug uptake enhancement factor into nail clippings. Moreover, zone of inhibitions for ITZ-nPEVs (27.0 ± 0.25 mm) and CMFG-ITZ-nPEVs (33.2 ± 0.09 mm) against Candida albicans were significantly greater than that of Itrostred gel (22.9 ± 0.44 mm). For clinical investigation on onychomycotic patients, a nail penetration enhancer containing ITZ-nPEV-loaded CMFG gel presents a highly promising approach.

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“…The determination of mean particle size, polydispersibility index (PDI), and zeta potential (ZP) of drug and SCs was measured through dynamic dispersion of the light technique using the Zetasizer (Malvern Instruments Ltd., Nano ZS90, Malvern, UK) [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

Preparation of Soluble Complex of Curcumin for the Potential Antagonistic Effects on Human Colorectal Adenocarcinoma Cells

Mohamed¹,

Alqahtani

Khan

et al. 2021

Pharmaceuticals

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This study was designed to investigate the effects of curcumin (CMN) soluble complex (SC) prepared by melt casting (HM) and hot-melt extrusion (HME) technology. Phase solubility (PS) study, in silico molecular modeling, aqueous solubility, drug release, and physicochemical investigation including a novel dyeing test was performed to obtain an optimized complex by a central composite design (CCD). The results show that the HME-SC produces better improvements towards solubility (0.852 ± 0.02), dissolution (91.87 ± 0.21 % at 30 min), with an ideal stability constant (309 and 377 M−1 at 25 and 37 °C, respectively) and exhibits AL type of isotherm indicating 1:1 stoichiometry. Intermolecular hydrogen bonding involves the formation of SC, which does not undergo any chemical modification, followed by the complete conversion of the amorphous form which was identified by XRD. The in vitro cytotoxicity showed that IC50 was achieved in the SW480 (72 µM.mL−1) and Caco-2 (40 µM.mL−1) cells while that of pure CMN ranged from 146 to 116 µM/mL−1. Apoptosis studies showed that cell death is primarily due to apoptosis, with a low rate of necrosis. In vivo toxicity, confirmed by the zebrafish model, exhibited the safety of the HME-SC. In conclusion, the HME-SC potentially enhances the solubility and cytotoxicity to the treatment of colorectal cancer (CRC).

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Human Hair Keratin Composite Scaffold: Characterisation and Biocompatibility Study on NIH 3T3 Fibroblast Cells

Cited by 32 publications

References 46 publications

Development of Atorvastatin Calcium Biloaded Capsules for Oral Administration of Hypercholesterolemia

Development of Atorvastatin Calcium Biloaded Capsules for Oral Administration of Hypercholesterolemia

The Unique Carboxymethyl Fenugreek Gum Gel Loaded Itraconazole Self-Emulsifying Nanovesicles for Topical Onychomycosis Treatment

Preparation of Soluble Complex of Curcumin for the Potential Antagonistic Effects on Human Colorectal Adenocarcinoma Cells

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