Human Hepatoma Cell Lines

Alexander, J. J.

doi:10.1007/978-4-431-68349-0_4

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…We previously characterized HBVassociated chromosomal translocations in HCC cell lines and validated most of them using spectral karyotyping [28]. At the time, it was unclear whether HBV-associated chromosomal translocations would be translatable to patient samples, or whether these translocations were an artifact of long-term culturing [36]. In addition, a recent study in HCC samples, also demonstrated the existence of HBVassociated chromosomal translocations in patients [37].…”

Section: Discussionmentioning

confidence: 99%

Targeted long-read sequencing reveals clonally expanded HBV-associated chromosomal translocations in patients with chronic hepatitis B

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Targeted long-read sequencing reveals clonally expanded HBV-associated chromosomal translocations in patients with chronic hepatitis B

et al. 2022

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“…After washing six times for 5 minutes with the washing buffer, antigen-antibody reaction was detected using an enhanced chemiluminescence (Amersham), followed by exposure to an autoradiography film (Kodak, Tokyo, Japan) for 5 to 30 seconds. A T-cell line, Jurkat, 39 was used as a positive control for bcl-2 expression, and PLC/PRF/5 40 and HepG2 41 were used as other controls as HCC cells. Because the enhanced chemiluminescence method enhances the antigen-antibody reaction signal, the treated and untreated samples were diluted 1:2 and 1:4 to show if the rise in Bcl-2 and p21 WAF-1 are caused by a true induction by butyrate treatment.…”

Section: Fluorographic Detection Of Apoptosis-associated Nucleic Altementioning

confidence: 99%

Loss of butyrate-induced apoptosis in human hepatoma cell lines HCC-M and HCC-T having substantial Bcl-2 expression

et al. 1998

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We have demonstrated that sodium butyrate induces differentiation in human hepatoma cells; however, recent studies have shown that this agent causes apoptosis in some types of cancer cells. In this study, we examined whether sodium butyrate causes apoptosis in the human hepatoma cell lines, HCC-M and HCC-T. The growth of human hepatoma cells was dose-dependently reduced by sodium butyrate. Flow cytometric analysis showed cell-cycle arrest at the G 1 phase in the sodium butyrate-treated cells. Apoptotic change was never found in treated cells at concentration levels of less than 5 mmol/L. Sodium butyrate decreased p53 expression and increased p21 WAF-1 expression in HCC-T and HCC-M cells having the wild-type p53 gene. Western blot analysis showed that Bcl-2 was expressed in the HCC-T and HCC-M cells, and its expression was increased after exposure to sodium butyrate. Antisense oligodeoxynucleotide against bcl-2 easily caused apoptosis. These results indicate that sodium butyrate hardly induces apoptotic change in the human hepatoma cell lines, HCC-T and HCC-M, with the increase of Bcl-2 expression. Cellcycle arrest in the G 1 phase caused by sodium butyrate was suggested to be induced by the increase in p21 WAF-1 expression, but this change did not link with the p53 increase.

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“…Four cell lines were used to generate the 3D liver cancer models. HepT1 (Pietsch et al, 1996) and HUH6 (Japanese Collection of Research Bioresources, Osaka, Japan) are HB cell lines, HepG2 (ATCC, Manassas, United States) is a cell line of a HB with hepatocellular carcinoma (HCC)-like features (López-Terrada et al, 2009), and HUH7 is a well differentiated HCC cell line (He et al, 1984;Alexander, 1987). All cell lines were cultured in RPMI 1640 (Life Technologies, Carlsbad, CA, United States), supplemented with 10% (v/v) fetal bovine serum (FBS, Life Technologies) and 1% Pen/Strep in a humidified chamber containing 5% CO 2 at 37 °C.…”

Section: Cell Culturementioning

confidence: 99%

Establishing a three-dimensional scaffold model of hepatoblastoma

Weigl,

Demir,

Schmid

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Emerging technologies such as three-dimensional (3D) cell culture and the generation of biological matrices offer exciting new possibilities in disease modelling and tumour therapy. The paucity of laboratory models for hepatoblastoma (HB), the most prevalent malignant liver tumour in children, has hampered the identification of new treatment options for HB patients. We aimed to establish a reliable 3D testing platform using liver-derived scaffolds and HB cell lines that reflect the heterogeneous biology of the disease so as to allow reproducible preclinical research and drug testing.Methods: In a sequence of physical, chemical and enzymatic decellularisation techniques mouse livers were stripped off all cellular components to obtain a 3D scaffold. HB cell lines were then seeded onto these scaffolds and cultivated for several weeks.Results: Our newly generated biological scaffolds consist of liver-specific extracellular matrix components including collagen IV and fibronectin. A cultivation of HB cell lines on these scaffolds led to the formation of 3D tumour structures by infiltration into the matrix. Analyses of drug response to standard-of-care medication for HB showed reliable reproducibility of our stocked models.Discussion: Our HB models are easy-to-handle, producible at large scale, and can be cryopreserved for ready-to-use on-demand application. Our newly generated 3D HB platform may therefore represent a faithful preclinical model for testing treatment response in precision cancer medicine.

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Human Hepatoma Cell Lines

Cited by 9 publications

References 38 publications

Targeted long-read sequencing reveals clonally expanded HBV-associated chromosomal translocations in patients with chronic hepatitis B

Targeted long-read sequencing reveals clonally expanded HBV-associated chromosomal translocations in patients with chronic hepatitis B

Loss of butyrate-induced apoptosis in human hepatoma cell lines HCC-M and HCC-T having substantial Bcl-2 expression

Establishing a three-dimensional scaffold model of hepatoblastoma

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