Human herpesvirus 8 variants in sarcoid tissues

Alberti, L Di; Piatteli, Adriano; Artese, Luciano; Favia, Gianfranco; Porter, Stephen; Scully, Crispian; Ngui, Siew-Lin; Teo, Chong–Gee; Patel, Sushil; Saunders, Nicholas A.

doi:10.1016/s0140-6736(97)10102-7

Cited by 150 publications

(75 citation statements)

References 33 publications

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“…0.007%). In comparison, 25 out of 6,930 nucleotides in data for sarcoid and other samples accumulated by Di Alberti et al (1997aAlberti et al ( , 1997b and 27 out of 5,040 nucleotides in the MM data reported by Ma et al (2000) represent changes outside of the six diagnostic polymorphic positions found within their 233-bp region. In other words, the data in both studies contained sporadic variations at a rate of 0.4 to 0.5%, which is 70-fold greater than we observed, and implies artifactual sequencing errors from cloned PCR products (see below).…”

Section: Levels Of Sporadic Versus Subtype-specific Orf26 Variabilitymentioning

confidence: 86%

“…This has been used successfully to confirm the presence of KSHV DNA in KS, PEL or MCD tissue samples, and in PBMC or saliva from immunosuppressed AIDS or KS patients (Moore and Chang,1995; Cesarman,1995; Whitby,1995; Soulier,1995;Marchioli, 1996; Huang,1995). However, some other clinical or epidemiological studies have claimed detection of KSHV DNA by PCR in other disease conditions, such as multiple myeloma (MM), sarcoid, primary pulmonary hypertension, pemphigus, Bowen's disease, or Kikuchi disease, and in saliva or urogenital specimens from seronegative patients (Luppi,1997;Chauhan, 1999; Hsu,2001; Gao,1999; Said,1997a; Tisdale,1998; Monini,1996; Rettig,1997;Di Alberti, 1997b; Di Alberti,1997a; Cool,2003; Agbalika,1998; Brousset,2000; Boralevi,1998;Said, 1996; Kikuta,1997; Tasaka,1997; Brousset,1997; Huh,1998; Sjak-Shie,1999; Berenson and Vescio,1999; Nishimoto,1997; Memar,1997; Merelli,1997; Said,1997b). For the most part those claims have not been reproducible by other investigators, or by other techniques, and have not gained wide acceptance (Pan,2001; Tarte,1999; Moore and Chang,1998;Moore, 1998; Henke-Gendo,2005; Corbellino,1999).…”

Section: Use Of Pcr Dna Data In Clinical and Epidemiological Studies mentioning

confidence: 99%

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Reflections on the interpretation of heterogeneity and strain differences based on very limited PCR sequence data from Kaposi's sarcoma-associated herpesvirus genomes

Zong

Arav‐Boger

Alcendor

et al. 2007

Journal of Clinical Virology

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Background and PurposeA human gamma class herpesvirus within the rhadinovirus family known as Kaposi's sarcomaassociated herpesvirus (KSHV) or human herpesvirus 8 (HHV8) is now fully accepted as the etiological agent of both Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL), as well as of some cases of multicentric Castleman's didease (MCD). The rates of these diseases are greatly increased in certain AIDS subpopulations (epidemic form), less so in organ transplant patients (iatrogenic form), and they also occur in rare non-AIDS associated cases, usually in elderly men (classical form). KSHV DNA can be detected in virtually all KS lesions and PEL cell lines, and in many MCD biopsies, and often also in PBMC or saliva from these same patients, from asymptomatic dual HIV and KSHV seropositive AIDS patients, and in febrile children in Africa undergoing primary KSHV infections. However, except in subSaharan Africa (>50%) and parts of the Mediterranean and Middle East (15%), most human populations show only 1 to <3% seropositivity rates and KSHV DNA is rarely detectable in PBMC and saliva samples from healthy individuals.The purpose of this review is two-fold, 1) to critically evaluate controversial claims linking KSHV to novel disease conditions; and 2) to evaluate claims linking pathological significance to specific sequence variants of KSHV. Our perspective is based on extensive experience with PCR DNA sequencing of both constant and variable gene regions at multiple loci from KSHV (and human cytomegalovirus [HCMV]) in clinical samples obtained from ethnically and geographically diverse human populations. KSHV genomes display significant levels of genetic polymorphism that are both interesting and informative. Variability observed within the most commonly employed ORF26 PCR locus will be evaluated based on the results presented in the accompanying paper, which describes the detection of eight distinct clustered nucleotide polymorphism patterns in an expanded version of this locus (Zong and Hayward, 2007). The significance of KSHV subtypes and DNA sequence polymorphisms will be assessed in the context of global clustering patterns and the overall levels of variability observed Corresponding Author: Gary S. Hayward, Phone: (410) Fax: (410) 955-8685, E Mail: ghayward@jhmi.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Use of PCR DNA Data in Clinical and Epidemiological Studies of KSHV InfectionEvidence for KSHV infection is usually based upon detection of antibodies by serological assays or detection of viral DNA by PCR or both. However, ther...

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Section: Levels Of Sporadic Versus Subtype-specific Orf26 Variabilitymentioning

confidence: 86%

Section: Use Of Pcr Dna Data In Clinical and Epidemiological Studies mentioning

confidence: 99%

Reflections on the interpretation of heterogeneity and strain differences based on very limited PCR sequence data from Kaposi's sarcoma-associated herpesvirus genomes

Zong

Arav‐Boger

Alcendor

et al. 2007

Journal of Clinical Virology

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“…HHV-8 has been associated with sarcoidosis in Italian patients 6 , but not in French patients 107 . Furthermore, a serological assay (ELISA) detected antibodies in only 3 out 15 (20%) of Swiss patients 108 .…”

Section: Other Conditionsmentioning

confidence: 96%

“…There is strong evidence that HHV-8 not only is the causative agent of KS 2 but also of primary effusion lymphoma (PEL) (also termed body cavity-based lymphomas (BCBL) 3 and multicentric Castleman disease (MCD) 4 . Human herpesvirus 8 has also been tenuously etiologically linked with multiple myeloma 5 and nonneoplastic disorders such as sarcoidosis 6 .…”

Section: Introductionmentioning

confidence: 99%

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Leão¹,

Caterino–de–Araujo

Porter

et al. 2002

Rev. Hosp. Clin.

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OBJECTIVE: To review the current literature on human herpesvirus 8 with particular attention to the aspects related to the etiopathogenesis of Kaposi's sarcoma. MATERIALS AND METHODS: The authors searched original research and review articles on specific aspects of human herpesvirus 8 infection, including virology, epidemiology, transmission, diagnosis, natural history, therapy, and Kaposi's sarcoma etiopathogenesis. The relevant material was evaluated and reviewed. RESULTS: Human herpesvirus 8 is a recently discovered DNA virus that is present throughout the world but with major geographic variation. In the Western world, the virus, transmitted mainly by means of sexual contact, is strongly associated with Kaposi's sarcoma and body cavity-based lymphoma and more controversially with multiple myeloma and other non-proliferative disorders. There is no specific effective treatment, but HIV protease inhibitors may play an indirect role in the clearance of human herpesvirus 8 DNA from peripheral blood mononuclear cells of HIV-infected patients. Human herpesvirus 8 DNA is present in saliva, but there are as yet no documented cases of nosocomial transmission to health care workers. The prevalence of human herpesvirus 8 among health care workers is probably similar to that in the general population. CONCLUSION: Human herpesvirus 8 appears to be, at least in Western Europe and United States, restricted to a population at risk of developing Kaposi's sarcoma. Human herpesvirus 8 certainly has the means to overcome cellular control and immune responses and thus predispose carriers to malignancy, particularly Kaposi's sarcoma. The wide diffusion of Human herpesvirus 8 in classic Kaposi's sarcoma areas appears to represent an important factor in the high incidence of the disease. However, additional co-factors are likely to play a role in the development of Kaposi's sarcoma.

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“…HHV-8 has been also associated with body cavity B-cell lymphoma, benign lymphoproliferative disease, angiosarcoma of the face, angiolymphoid hyperplasia with eosinophilia, multicentric Castleman's disease (Marchioli et al, 1996;Foreman et al, 1997), and sarcoidosis (DiAlberti et al, 1997). There have also been isolated cases of HHV-8 DNA sequences in patients without immunosuppression or KS (Memar et al, 1997;Trovato et al, 1999), although there is a general agreement that HHV-8 pathogenic effects are enhanced by immunosuppression.…”

Section: Gómez-román Et Almentioning

confidence: 99%

Presence of Human Herpesvirus-8 DNA Sequences and Overexpression of Human IL-6 and Cyclin D1 in Inflammatory Myofibroblastic Tumor (Inflammatory Pseudotumor)

Gómez-Román

Ocejo-Vinyals

Sánchez-Velasco

et al. 2000

Laboratory Investigation

118

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SUMMARY:Inflammatory myofibroblastic tumor (IMT) is composed of myofibroblasts, plasma cells, and lymphocytes.Cytokines are possibly involved in its pathogenesis. Human herpesvirus-8 (HHV-8) encodes cell cycle regulatory and signaling proteins. A combination of nested PCR with several negative controls and Southern blot methods showed the presence of HHV-8 DNA in seven cases of IMT. Additionally, strong expression was demonstrated by in situ hybridization in many tumoral nuclei. Most of the myofibroblasts in all of the cases were immunoreactive for human IL-6 and cyclin D1. These cytokines probably have a paracrine action and may sustain myofibroblastic growth. HHV-8 could play an essential role in triggering IMT development by a local reactivation of viral lytic replication. The relationship between HHV-8 and immunosuppression status as the only associated cause for tumorigenesis should be revised. (Lab Invest 2000, 80:1121-1126.

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Human herpesvirus 8 variants in sarcoid tissues

Cited by 150 publications

References 33 publications

Reflections on the interpretation of heterogeneity and strain differences based on very limited PCR sequence data from Kaposi's sarcoma-associated herpesvirus genomes

Reflections on the interpretation of heterogeneity and strain differences based on very limited PCR sequence data from Kaposi's sarcoma-associated herpesvirus genomes

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Presence of Human Herpesvirus-8 DNA Sequences and Overexpression of Human IL-6 and Cyclin D1 in Inflammatory Myofibroblastic Tumor (Inflammatory Pseudotumor)

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