Human immature dental pulp stem cells’ contribution to developing mouse embryos: production of human/mouse preterm chimaeras

Abstract

hIDPSC showed biological compatibility with the mouse host environment and could survive, proliferate and contribute to the inner cell mass as well as to the trophoblast cell layer after introduction into early mouse embryos (n = 28), which achieved the hatching stage following 24 and 48 h in culture. When transferred to foster mice (n = 5), these blastocysts with hIDPSC (n = 57) yielded embryos (n = 3) and foetuses (n = 6); demonstrating presence of human cells in various organs, such as brain, liver, intestine and hearts, of the human/mouse chimaeras. We verified whether hIDPSC would also be able to differentiate into specific cell types in the mouse environment. Contribution of hIDPSC in at least two types of tissues (muscles and epithelial), was confirmed. We showed that hIDPSC survived, proliferated and differentiated in mouse developing blastocysts and were capable of producing human/mouse chimaeras.

Tooth Organ Engineering: Biological Constraints Specifying Experimental Approaches

“…The stem cells of the dental pulp (Table 3, SHEDs, DPSCs or SCAPs) constitute per se an heterogenous cell population which, depending on the isolation, selection and culture procedures may display different features and degrees of stemness (Table 3, Bakopoulou et al, 2011;Huang et al, 2006). It is remarkable that although the majority of studies involved cells isolated by enzymatic dissociation of the pulpal tissue, the most immature dental pulp stem cells were obtained from explants culture (Siqueira da Fonseca et al, 2009 Determining which type of DPSCs is the best suited for tooth engineering is therefore an essential question. The purification of a homogeneous DPSCs population is not possible due to a lack of known specific markers.…”

Dental stem cells for tooth regeneration and repair

“…The cells could survive, proliferate and contribute to the inner cell mass and the trophoblast cell layer after introduction into early mouse embryos. When transferred to foster mice (n = 5), these blastocysts with human pulp immature stem cells (n = 57) yielded embryos (n = 3) and foetuses (n = 6); demonstrating presence of human cells in various organs, such as brain, liver, intestine and hearts, of the human/mouse chimaeras [24].…”

Potential feasibility of dental stem cells for regenerative therapies: stem cell transplantation and whole-tooth engineering

“…They express several pluripotent cell markers, including Oct-4 and Nanog, and can differentiate under chemically defined conditions into multiple cell lineages: neurogenic, chondrogenic, osteogenic, and myogenic. Moreover, IDPSCs contributed to mouse embryogenesis when the cells were introduced into mouse developing blastocysts, which developed into human/mouse chimera embryos and fetuses in foster mice (the chimeras were collected from the mothers before birth according to ethical recommendations) [37]. These findings demonstrate that human IDPSCs behave as pluripotent stem cells like ES/iPS cells in vitro and in vivo, suggesting that somatic stem cells that originate from human teeth have great potential as an alternative stem cell population to ES/iPS cells.…”