Human Immunodeficiency Virus (HIV)

Amini, Ali; Andersson, Monique; Gupta, Ravindra; Angus, Brian

doi:10.1002/9781119260363.ch12

Cited by 1 publication

(1 citation statement)

References 143 publications

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“…Chronic infections, like chronic viral hepatitis, tuberculosis, and human immunodeficiency virus (HIV) infection, are widely spread and classified as the most infectious disease killers. These features express the consequences of chronic infections for the global health ( 61 – 63 ).…”

Section: Crispr-based Diagnostic Systems For Detecting Infectious Diseasesmentioning

confidence: 99%

SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases

2021

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Infectious diseases are one of the most intimidating threats to human race, responsible for an immense burden of disabilities and deaths. Rapid diagnosis and treatment of infectious diseases is a better understanding of its pathogenesis. According to WHO, the ideal approach for detecting foreign pathogens should be rapid, specific, sensitive, instrument-free and cost-effective. Nucleic acid pathogen detection methods, typically PCR have numerous limitations, such as highly sophisticated equipments, reagents, and trained personnel rely on well-established laboratories beside time-consuming. Thus, there is a crucial need to develop novel nucleic acid detection tools with rapid, specific, sensitive, and cost-effective, particularly ones that can be used for versatile point-of-care diagnostic applications. Two new methods exploit on unpredicted in vitro properties CRISPR-Cas effectors, turning activated nucleases into basic amplifiers of a specific nucleic-acid binding event. These effectors are attached with a diversity of reporters and utilized in tandem with present of isothermal amplification approaches to create sensitive identification in multiple field deployable formats. Although still in their beginning, yet SHERLOCK and DETECTR technologies are potential methods for rapid detection and identification of infectious disease, with ultra-sensitive tests that don't require a lot of complicated processing. This review described SHERLOCK and DETECTR technologies beside their properties, functions, and perspectives to become the ultimate diagnostic tools for diagnosing infectious diseases and curbing disease outbreaks.

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Section: Crispr-based Diagnostic Systems For Detecting Infectious Diseasesmentioning

confidence: 99%