Human immunodeficiency virus type 2 (HIV-2) env gene analysis: prediction of glycoprotein epitopes important for heterotypic neutralization and evidence for three genotype clusters within the HIV-2a subtype

Alterations in the V1/V2 Domain of HIV-2CBL24Glycoprotein 105 Correlate with an Extended Cell Tropism

“…Such truncation of gp41 has been reported in a primary HIV-2 isolate 10 but occurs more com monly after passage in continuous cell lines such as Molt-4 cl.8, 3,11 and SupT1. 12 Truncation has been associated with increased syncytium induction and reduced virion release.…”

“…env genes were amplified by nested polymerase chain reaction (PCR), using the primers H2POLF (CARGGAGTAGTRGAAGCAATGAA) and H2LTRR (TGC-TTCTAAYTGGCAGCTTTATT) and prim ers H2ENVF (GACTCCGCGGAAAACTAAGRCTCATYCGTCTYCTG-CAYCAGA) and H2ENVR (TTGGTGTGTCCGGAACYC-CYACTAGGTCATC), as described. 3 Regions corresponding to nucleotides (nt) 4499-9671 and nt 6096-8869 of HIV-2 R O D were amplified. Purified env genes were sequenced directly on an ABI-377 sequencer using dye terminator cycle sequencing Ready Reaction kits (Perkin-Elme r, Foster City, CA) and a bank of primers.…”

“…Purified env genes were sequenced directly on an ABI-377 sequencer using dye terminator cycle sequencing Ready Reaction kits (Perkin-Elme r, Foster City, CA) and a bank of primers. 3 Sequences were analyzed using GCG and GDE programs ( Fig. 1 4 ).…”

A neutralizing monoclonal antibody previously mapped exclusively on human immunodeficiency virus type 1 gp41 recognizes an epitope in p17 sharing the core sequence IEEE

“…Therefore, further characterization of the 735-752 gp41 neutralizing motif may become very important for future work on subunit vaccines based on gp41. In addition, this epitope has been predicted to be a potential target for heterotypic neutralization of HIV-1 and HIV-2 strains (5). The possibility of inducing such heterotypic neutralization has been recently supported by the induction in rhesus macaques of cross-protection by HIV-1 recombinant poxvirus vaccines against HIV-2 infection (1).…”

Immune Responses againstHIV-2

“…4 The classification of a phylogenetic tree of primate lentiviruses leads to a first level of different lineages, and the second level of classification is to identify subgroups within each of these clusters. [5][6][7][8][9] HIV-2 has been divided into several different subtypes, and nearly all of them have been identified in West Africa. More recently, epidemic spreading of HIV-2 subtype A isolates was demonstrated in India.…”