Human induced pluripotent stem cell-derived neural crest stem cells integrate into the injured spinal cord in the fetal lamb model of myelomeningocele

Saadai, Payam; Wang, Aijun; Nout, Yvette S.; Downing, Timothy L.; Lofberg, Katrine; Beattie, Michael S.; Bresnahan, Jacqueline C.; Li, Song; Farmer, Diana L.

doi:10.1016/j.jpedsurg.2012.10.034

Cited by 79 publications

(50 citation statements)

References 16 publications

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“…To detect human cells, we used the human-specific antibody, NUMA204445. NUMA staining was detected in all cells within a cross-section of a 20-week human fetal testis; in contrast, NUMA antibodies had no reactivity in cells from the un-injected mouse testis (Fig 5B).…”

Section: Resultsmentioning

confidence: 99%

Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies

Dominguez

Chiang

Sukhwani

et al. 2014

Sci Rep

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Turner syndrome is caused by complete or partial loss of the second sex chromosome and is characterized by spontaneous fetal loss in >90% of conceptions. Survivors possess an array of somatic and germline clinical characteristics. Induced pluripotent stem cells (iPSCs) offer an opportunity for insight into genetic requirements of the X chromosome linked to Turner syndrome. We derived iPSCs from Turner syndrome and control individuals and examined germ cell development as a function of X chromosome composition. We demonstrate that two X chromosomes are not necessary for reprogramming or maintenance of pluripotency and that there are minimal differences in gene expression, at the single cell level, linked to X chromosome aneuploidies. Formation of germ cells, as assessed in vivo through a murine xenotransplantation model, indicated that undifferentiated iPSCs, independent of X chromosome composition, are capable of forming germ-cell-like cells (GCLCs) in vivo. In combination with clinical data regarding infertility in women with X chromosome aneuploidies, results suggest that two intact X chromosomes are not required for human germ cell formation, qualitatively or quantitatively, but rather are likely to be required for maintenance of human germ cells to adulthood.

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Section: Resultsmentioning

confidence: 99%

Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies

Dominguez

Chiang

Sukhwani

et al. 2014

Sci Rep

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“…Neurospheres derived from human iPSCs have been transplanted into the spinal cord injury of mice models where they differentiated into three major neural lineages, neurons, astrocytes, and oligodendrocytes and promoted a significant increase in functional recovery compared to control animals [8]. Another study differentiated human iPSCs into neural crest cells in vitro and then transplanted these cells into a fetal lamb model of spinal cord injury [14]. These cells survived and differentiated into neurons after transplantation with no tumor formation observed.…”

Section: Introductionmentioning

confidence: 99%

Electrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human‐induced pluripotent stem cells

Mohtaram

King

et al. 2014

J Biomedical Materials Res

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In this study, we investigated the effect of micro and nanoscale scaffold topography on promoting neuronal differentiation of human induced pluripotent stem cells (iPSCs) and directing the resulting neuronal outgrowth in an organized manner. We used melt electrospinning to fabricate poly (ε‐caprolactone) (PCL) scaffolds with loop mesh and biaxial aligned microscale topographies. Biaxial aligned microscale scaffolds were further functionalized with retinoic acid releasing PCL nanofibers using solution electrospinning. These scaffolds were then seeded with neural progenitors derived from human iPSCs. We found that smaller diameter loop mesh scaffolds (43.7 ± 3.9 µm) induced higher expression of the neural markers Nestin and Pax6 compared to thicker diameter loop mesh scaffolds (85 ± 4 µm). The loop mesh and biaxial aligned scaffolds guided the neurite outgrowth of human iPSCs along the topographical features with the maximum neurite length of these cells being longer on the biaxial aligned scaffolds. Finally, our novel bimodal scaffolds also supported the neuronal differentiation of human iPSCs as they presented both physical and chemical cues to these cells, encouraging their differentiation. These results give insight into how physical and chemical cues can be used to engineer neural tissue. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2591–2601, 2015.

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“…Induced pluripotent stem cells (iPSC) are amongst the most promising cell sources for tissue engineering because iPSC show high multi-differentiation and self-renewal capabilities [70,71]. Recently, several studies have reported the successful generation of NCSC from iPSC [72][73][74][75][76]. Interestingly, iPSC-derived NCSC showed no tumor formation after their transplantation into nude mice and could differentiated into odontoblast-like cells that expressed odontoblast markers, DSP, Pax9, Msx1, and Lhx6 [76].…”

Section: Future Perspectivesmentioning

confidence: 99%

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Tomokiyo

Hynes

Gronthos

et al. 2015

Curr Oral Health Rep

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Human induced pluripotent stem cell-derived neural crest stem cells integrate into the injured spinal cord in the fetal lamb model of myelomeningocele

Cited by 79 publications

References 16 publications

Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies

Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies

Electrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human‐induced pluripotent stem cells

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

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