Human interleukin‐1 receptor antagonist is expressed in liver

Steinkasserer, Alexander; Estaller, Cornelia; Weiß, Elisabeth H.; Sim, Robert B.

doi:10.1016/0014-5793(92)81146-d

Cited by 15 publications

(8 citation statements)

References 12 publications

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“…Our data establish that only sIL-1Ra, and not icIL-1Ra, is transcribed in hepatic cells, consistent with the results of earlier studies (37). We also observed, however, the presence of a lower molecular form of IL-1Ra in HepG2 cell lysates.…”

Section: Discussionsupporting

confidence: 93%

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Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein.

Gabay

Smith²,

Eidlen³

et al. 1997

J. Clin. Invest.

285

197

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Interleukin 1 receptor antagonist (IL-1Ra) levels are elevated in the blood of patients with a variety of infectious, immune, or traumatic conditions. To examine whether IL1Ra is produced by liver cells with characteristics resembling an acute-phase protein, human primary hepatocytes isolated from liver biopsies and HepG2 hepatoma cells were stimulated with IL-1 ␤ , IL-6, and TNF ␣ . IL-1Ra was present in the supernatants of both cells, with production significantly enhanced by IL-1 ␤ , and by the combination of IL-1 ␤ and IL-6. The term IL-1Ra refers to two different proteins encoded by the same gene, but generated by alternative splicing of two different first exons. One isoform is secreted (17-kD sIL-1Ra), and the other isoform remains in the cytoplasm (18-kD icIL-1Ra). By Western blot analysis, the supernatants of human hepatoma (HepG2)

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Section: Discussionsupporting

confidence: 93%

“…The IL-1Ra mRNA has been described in HepG2 cells, and IL-1Ra production by hepatocytes was observed during bacteria-induced hepatitis in mice (37,38). The regulation of IL-1Ra production by IL-1 and IL-6 in human primary hepatocytes and HepG2 cells described herein, however, clearly classifies IL-1Ra as an APP.…”

Section: Discussionmentioning

confidence: 61%

Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein.

Gabay

Smith²,

Eidlen³

et al. 1997

J. Clin. Invest.

285

197

View full text Add to dashboard Cite

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“…1C). Previous reports have indicated that hepatocytes express a soluble, secreted form of IL1RN, whereas expression of an intracellular form is negligible (26,32). Therefore, the detected band appears to represent soluble IL1RN.…”

Section: Identification Of Pgc-1␣-regulated Hepatic Inflammatorymentioning

confidence: 91%

Energy-sensing Factors Coactivator Peroxisome Proliferator-activated Receptor γ Coactivator 1-α (PGC-1α) and AMP-activated Protein Kinase Control Expression of Inflammatory Mediators in Liver

Buler

Aatsinki

Skoumal

et al. 2012

Journal of Biological Chemistry

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Background: Metabolic disorders are associated with chronic inflammation. Results: Energy-sensing factor PGC-1␣ regulates cytokine expression in hepatocytes. PGC-1␣, AMPK, and metformin induce expression of interleukin 1 receptor antagonist. Conclusion: PGC-1␣ and AMPK mediate effects of fasting, physical exercise, and antidiabetic drug metformin on hepatic inflammatory gene expression. Significance: PGC-1␣ and AMPK are regulatory interlinks between energy homeostasis and inflammation.

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“…62 IL-1Ra is expressed in the liver and in hepatoma cells, suggesting a role in regulating IL-1 activity in the liver. 63 In summary, IL-1Ra attenuates the acute phase response both in vivo and in vitro, although species differences may exist.…”

Section: Soluble Cytokine Receptors and Receptor Antagonistsmentioning

confidence: 99%

Cytokines and the hepatic acute phase response

1997

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The acute phase response is an orchestrated response to tissue injury, infection or inflammation. A prominent feature of this response is the induction of acute phase proteins, which are involved in the restoration of homeostasis. Cytokines are important mediators of the acute phase response. Uncontrolled and prolonged action of cytokines is potentially harmful, therefore mechanisms exist which limit the activity of cytokines; these include soluble cytokine receptors and receptor antagonists. The cytokine signal is transmitted into the cell via membrane‐bound receptors. Different intracellular signalling pathways are activated by different cytokine‐receptor interactions. Eventually, cytokine‐inducible transcription factors interact with their response elements in the promotor region of acute phase genes and transcription is induced. Systemic inflammation results in a systemic acute phase response. However, local inflammatory or injurious processes in the liver may also induce an acute phase response, for example after partial hepatectomy and during hepatic fibrosis. The acute phase proteins induced in these conditions probably act to limit proteolytic and/or fibrogenic activity and tissue damage. The possible function of the acute phase protein α2‐macroglobulin in hepatic fibrosis is discussed in some detail. © 1997 John Wiley & Sons, Ltd.

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Human interleukin‐1 receptor antagonist is expressed in liver

Cited by 15 publications

References 12 publications

Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein.

Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein.

Energy-sensing Factors Coactivator Peroxisome Proliferator-activated Receptor γ Coactivator 1-α (PGC-1α) and AMP-activated Protein Kinase Control Expression of Inflammatory Mediators in Liver

Cytokines and the hepatic acute phase response

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