Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells.

Goodwin, Raymond G.; Lupton, Stephen D.; Schmierer, Ann E.; Hjerrild, Kathryn; Jerzy, R; Clevenger, William; Gillis, S; Cosman, David; Namen, Anthony E.

doi:10.1073/pnas.86.1.302

Cited by 251 publications

(132 citation statements)

References 22 publications

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“…IL-7 is secreted by stromal cells in red marrow, thymus, keratinocytes, dendritic cells, and endothelial cells (3). It binds to a heterodimeric receptor, IL-7R, made up of two glycosylated subunits: IL-7R␣ (CD127, 65 kDa) (4), specific to IL-7 and thymic stromal lymphopoietin (5), and the common ␥ chain (␥c, CD132, 56 kDa) shared by IL-2, 4, 7, 9, 15 and 21 receptors (2).…”

Section: Interleukin (Il)-7 Is the Main Homeostatic Regulator Of Cd4 mentioning

confidence: 99%

Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells

Tamarit

Bugault

Pillet

et al. 2013

Journal of Biological Chemistry

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Background: Interleukin-7 is the master regulator of T-cell proliferation. Results: IL-7 drives its receptor in a membrane microdomain that regulates phosphorylation of associated tyrosine kinases JAK1 and JAK3, anchors IL-7 receptor to cytoskeleton and regulates STAT5 phosphorylation and nuclear translocation. Conclusion: Membrane microdomains and cytoskeleton scaffold IL-7R-signalosomes and assist signaling protein transport. Significance: Transient membrane and cytoskeleton organization shapes IL-7-signaling mechanisms in CD4 T-cells.

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Section: Interleukin (Il)-7 Is the Main Homeostatic Regulator Of Cd4 mentioning

confidence: 99%

Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells

Tamarit

Bugault

Pillet

et al. 2013

Journal of Biological Chemistry

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“…However, we were also able to detect IL-7 protein secretion in 2/4 long-term established colon cancer cell lines that were devoid of any contaminating lymphoid cells, suggesting that colon cancer cells may indeed represent the source of IL-7 protein production. While this finding appears surprising at first glance, human IL-7 cDNA was first identified and subsequently cloned from a human hepatocarcinoma cell line SK-HEP-1 [35]. The physiological interaction of cytokines produced by epithelial cells or stromal cells with the immune system are poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Interleukin‐7 (IL‐7) in Colorectal Cancer: IL‐7 is Produced by Tissues from Colorectal Cancer and Promotes Preferential Expansion of Tumour Infiltrating Lymphocytes

et al. 1997

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Despite increasing survival rates for patients with colorectal cancer, additional treatment options are required, including active or passive immunotherapy for patients with metastatic disease. Freshly harvested colorectal cancer specimens and in vitro cultured colorectal cancer cell lines were examined for IL-7 protein secretion in order to examine the potential role of this cytokine in the interaction between tumour cells and the host immune system. Freshly harvested colorectal cancer specimens (21/21), or normal adjacent mucosa (3/3), as well as long-term established colorectal cancer cell lines (3/4) exhibited IL-7 mRNA expression as detected by RT-PCR and confirmed by Southern Blot analysis. Freshly harvested colorectal cancer tissue (16/18), or long-term established colorectal cancer cell lines (2/4) secreted in vitro IL-7 as detected by ELISA. In contrast, breast, pancreatic, or lung cancer cell lines, as well as several haematopoietic cancer cells lines, tested negative for IL-7 mRNA and protein. The authors tested different cytokines (IL-1b, IL-2, IL-7, or a combination of IL-1b/IL-7) in vitro for the ability to expand tumour -infiltrating T lymphocytes (TIL) from individual patients (n ¼ 9) with colorectal cancer. TIL populations were tested at day 14 after in vitro propagation for phenotypic analysis by FACS and for reactivity directed against NK and LAK sensitive target cells and autologous cancer cells as measured by cytotoxicity and cytokine release. TIL obtained from colorectal cancer lesions can be efficiently expanded in the presence of IL-7, some (3/9) of which appear to exhibit autologous tumour recognition as measured by cytolytic effector functions and by detection of IFNg and TNFa release. Detection of IL-7 mRNA expression in colorectal cancer, in normal mucosa adjacent to tumour, as well as the ability of colorectal cancer tissue to secrete IL-7, raises new questions about the biology of the host / tumour interactions in colorectal cancer.

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“…The interleukin-7 (IL-7) and IL-7 receptor (IL-7R) axis plays a crucial role in immune cell differentiation and survival; [1][2][3] genetic deletions in IL-7 or IL-7R lead to a severe combined immunodeficiency phenotype. 4,5 Both IL-7 and IL-7R have been shown to be alternatively spliced.…”

Section: Introductionmentioning

confidence: 99%

Increased (6 exon) interleukin-7 production after M. tuberculosis infection and soluble interleukin-7 receptor expression in lung tissue

et al. 2011

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Interleukin-7 (IL-7) and the IL-7 receptor (IL-7R) have been shown to be alternatively spliced in infectious diseases. We tested IL-7 and IL-7R splicing in a tuberculosis (TB)-vaccine/Mycobacterium tuberculosis (Mtb)-challenge model in non-human primates (NHPs). Differential IL-7 splicing was detected in peripheral blood mononuclear cells (PBMCs) from 15/15 NHPs showing 6 different IL-7 spliced isoforms. This pattern did not change after infection with virulent Mtb. We demonstrated increased IL-7 (6 exon) and IL-17 protein production in lung tissue along with concomitant decreased transforming growth factor-b (TGF-b) from NHPs (vaccinated with a recombinant BCG (rBCG)) who showed increased survival after Mtb challenge. IL-7 increased IL-17 and interferon-g (IFN-g) gene and protein expression in PBMCs. Mtb-infected NHPs showed differential IL-7R splicing associated with the anatomical location and tissue origin, that is, in lung tissue, hilus, axillary lymph nodes (LNs) and spleen. Differential splicing of the IL-7R was typical for healthy (non-Mtb infected) and for Mtb-infected lung tissue with a dominant expression of soluble IL-7R (sIL-7R) receptor lacking exon 6 (9:1 ratio of sIL-7R/cell-bound IL-7R). Differential ratios of cell-bound vs sIL-7R could be observed in hilus and axillary LNs from Mtb-infected NHPs with an inversed ratio of 1:9 (sIL-7R/cell-bound IL-7R) in spleen and PBMCs. Soluble IL-7R is exclusively present in lung tissue.

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Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells.

Cited by 251 publications

References 22 publications

Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells

Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells

Interleukin‐7 (IL‐7) in Colorectal Cancer: IL‐7 is Produced by Tissues from Colorectal Cancer and Promotes Preferential Expansion of Tumour Infiltrating Lymphocytes

Increased (6 exon) interleukin-7 production after M. tuberculosis infection and soluble interleukin-7 receptor expression in lung tissue

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