Human intestinal spirochetosis diagnosed with colonoscopy and analysis of partial 16S rDNA sequences of involved spirochetes

Kraatz, Wolfgang; Thunberg, Ulf; Pettersson, Bertil; Fellström, Claes

doi:10.1079/ahrr200121

Cited by 19 publications

(16 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study caused some controversy, as it implied not just that there were multiple strains and genetic clusters of B. aalborgi, but also, for the first time, that individuals could harbour multiple different strains of B. aalborgi, as well as related but uncharacterized spirochaetes. In a subsequent study, partial (207 bp) sequences of the 16S rRNA gene were amplified from colonic biopsies taken from 33 patients (Kraaz et al, 2001). Although the small size of the sequence did not allow robust phylogenetic analysis, the results broadly supported the existence of strain variation in B. aalborgi, as well as the presence of several clusters of B. aalborgi-like spirochaetes, which were identified in different individuals.…”

Section: Introductionmentioning

confidence: 61%

“…This lack of information has hampered attempts to find links between colonization with specific strains of B. aalborgi and the development of intestinal symptoms. To circumvent the problem of a lack of isolates, it is possible to use PCR to amplify B. aalborgi gene sequences directly from colonized tissue and/or faeces (Mikosza et al, 1999(Mikosza et al, , 2001aKraaz et al, 2001) and then to examine these sequences for heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Mikosza

Munshi

Hampson

2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

The purpose of this study was to investigate genetic variation in the anaerobic intestinal spirochaete Brachyspira aalborgi by partial sequencing of the 16S rRNA and NADH oxidase genes. The spirochaete is poorly cultivable; hence, only six isolates were available for analysis. Additional sequences were amplified from DNA extracted from fixed colorectal biopsies from 26 patients with histological evidence of intestinal spirochaetosis, and from the faeces of six non-human primates (NHP). Multiple biopsies from sites along the large intestine were tested from three of the 26 patients. Sequences from two biopsies were closely related to those of the spirochaete Brachyspira pilosicoli. Eight B. aalborgi-like 16S rDNA sequences were generated from the biopsies from the other 24 patients, and four from the NHP faeces. The B. aalborgi 16S rDNA sequences were divided into three clusters, 1, 2 and 4, with individual sequence similarities to the type strain ranging from 97 . 49 to 100 %. All human isolates of B. aalborgi were located in cluster 1, as was the sequence of the so-called 'Brachyspira ibaraki'. All four 16S rDNA sequences from the NHP faeces and the two NHP isolates of B. aalborgi were located in cluster 4, which was distinct. Cluster 4 may represent a novel Brachyspira species. Evidence for multiple strains of B. aalborgi or other Brachyspira species was found in biopsies from two patients. In the three individuals from whom multiple biopsies were amplified, the sequences at each intestinal site were the same, indicating the presence of one dominant strain.

show abstract

Section: Introductionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Mikosza

Munshi

Hampson

2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…This is the first study to provide an estimate of the prevalence of B. aalborgi in the general population of a developing country. Unfortunately, there have been no studies on the prevalence of B. aalborgi in developed countries, apart from in selected groups of patients who have undergone colorectal biopsy for underlying complaints of the large bowel [21,[25][26][27][28]. In the absence of this comparative data, it is not possible to determine whether the prevalence of colonization that was detected is unusually high or low, or whether it was influenced by factors peculiar to developing countries.…”

Section: Discussionmentioning

confidence: 99%

“…In recent studies, DNA has been extracted from colorectal biopsies taken from IS patients in Australia, Scandinavia and the United States, and amplified by polymerase chain reaction (PCR) or hybridized with oligonucleotide probes. Somewhat unexpectedly, these studies showed that B. aalborgi is much more commonly involved in IS in these developed countries than is B. pilosicoli [21,[25][26][27][28]. Similar studies have not been conducted on patients in developing countries, where B. pilosicoli is known to be common, due to difficulties in accessing suitable samples.…”

Section: Introductionmentioning

confidence: 99%

Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea estates in Assam, India

et al. 2004

View full text Add to dashboard Cite

SUMMARYThe prevalence of colonization with the anaerobic intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli was investigated in humans (n=316) and dogs (n=101) living on three tea estates in Assam, India. Colonization was detected using PCR on DNA from faeces. Nineteen (6 %) human faecal samples contained B. aalborgi DNA, 80 (25 . 3 %) contained B. pilosicoli DNA, and 10 (3 . 2%) contained DNA from both species. One canine sample contained DNA from B. pilosicoli. Significant factors for B. aalborgi colonization in logistic regression were : infection of family members with B. aalborgi (P<0 . 001), being a resident of Balipara (P=0 . 03), and use of water treatment (P=0 . 03). For B. pilosicoli, significant factors were : other family members being positive for B. pilosicoli (P<0 . 001), water obtained from a well (P=0 . 006), water treatment (P=0 . 03), and not having visited a doctor in the previous 12 months (P=0 . 03).

show abstract

“…The detection of Brachyspira spp. were performed as described by Kraatz et al [4]. Genus-specific primers targeting 16S rDNA were used in PCR and species determination was performed by sequencing of the PCR amplicons.…”

mentioning

confidence: 99%

Survey on the occurrence ofBrachyspiraspecies andLawsonia intracellularisin children living on pig farms

et al. 2006

View full text Add to dashboard Cite

SUMMARYThe occurrence of Brachyspira species and Lawsonia intracellularis was investigated by PCR analyses of faeces from 60 children living on European pig farms. In addition, 60 other children were included as controls. Two samples were positive for B. aalborgi but B. pilosicoli and L. intracellularis were not demonstrated.Two species of the genus Brachyspira, B. aalborgi and B. pilosicoli, have been associated with human intestinal spirochaetosis (HIS) [1,2]. The clinical significance of HIS is largely unknown. In Western countries, B. aalborgi seems to be more common with reported prevalences of for example, 7 . 9 % [3] or 70 % of submitted cases of HIS [4]. Brachyspira pilosicoli is more frequent in developing countries with reported prevalences of 15-23 % [2,3,5]. Further, B. pilosicoli and the intracellular rod Lawsonia intracellularis are major causes of enteric disease in young, growing pigs with 32% and 48 %, respectively, of the herds being infected [6,7]. The infection causes similar clinical signs and the microbes often occur concomitantly. L. intracellularis have been isolated in a wide range of other animal species, e.g. pig, hamster, horse, guinea pig, dog, lamb, calf, ferret, fox, deer, rabbit, rat, mouse, ratites, wild boar, wolf, giraffe, hedgehog, and primates. The broad host range raises the question whether natural cross-species transmission might occur. L. intracellularis belongs to the Desulfovibrionaceae family and in patients with ulcerative colitis an increased carriage of closely related bacteria has been demonstrated. The microbe causes proliferative enteropathy, characterized by crypt hyperplasia of immature cells. The lesions are morphologically similar to those found in patients with coeliac disease and hypotheses regarding a similar aetiology have been proposed. However, the bacterium has never been reported in humans [8][9][10].The aim of the present study was to investigate the occurrence of Brachyspira species and L. intracellularis in faeces from children living on European pig farms and compare the findings to those in children not living on pig farms.

show abstract

Human intestinal spirochetosis diagnosed with colonoscopy and analysis of partial 16S rDNA sequences of involved spirochetes

Cited by 19 publications

References 37 publications

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Analysis of genetic variation in Brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16S rRNA and NADH oxidase genes

Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea estates in Assam, India

Survey on the occurrence ofBrachyspiraspecies andLawsonia intracellularisin children living on pig farms

Contact Info

Product

Resources

About