Human KGF is FGF-Related with Properties of a Paracrine Effector of Epithelial Cell Growth

Finch, Paul W.; Rubin, Jeffrey S.; Miki, Toru; Ron, Dina; Aaronson, Stuart A.

doi:10.1126/science.2475908

Cited by 915 publications

(460 citation statements)

References 29 publications

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“…In the co-culture system, there is a very complicated network of autocrine and paracrine secretion like in vivo. The granulosa cells secrete some growth factors, such as fibroblast growth factor 2 (FGF2), fibroblast growth factor 7 (FGF7) and leukemia inhibitory factor (LIF) (Finch et al, 1989;Van Wezel et al, 1995;Nilsson et al, 2002). These growth factors can stimulate the developing embryo.…”

Section: Discussionmentioning

confidence: 99%

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Zhou

Shi

et al. 2008

Animal

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The aims of the study were to measure the mRNA expression of brain-derived neurotrophic factor (BDNF) in bovine oocytes and early embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA) and nuclear transfer (NT), and to investigate the effects of BDNF on the development of IVF and parthenogenetic embryos. Bovine oocytes matured in vitro for 22 h were in vitro fertilized or parthenogenetic activated. By reverse transcription-PCR and quantitative real-time PCR, we found that germinal vesicle (GV) oocytes, metaphase II (MII) oocytes, 4-cell and 8-cell embryos, morulae, and blastocysts were all shown to express mRNA for BDNF. The mRNA levels for BDNF gene were different in bovine oocytes and IVF embryos at different stages (P , 0.01), with the highest expression in MII oocytes and the lowest expression in 8-cell embryos. The mRNA for BDNF was highly expressed in the PA and IVF blastocysts compared to the NT blastocysts (P , 0.01). Supplementation of culture media with BDNF at the concentration of 40 mg/l caused a significant increase in the rates of in vitro-fertilized blastocyst formation (P , 0.05) and parthenogenetic blastocyst formation (P , 0.05). However, the rate of oocyte cleavage in BDNF groups was not significantly different from that in the BDNF-free control (P . 0.05) after IVF or PA. We have also investigated the effects of BDNF on the growth of granulosa cells, which were used for co-culture of bovine early embryos. The results revealed that supplementation of culture media with 20 mg/l BDNF promoted the growth of granulosa cells (P , 0.01). Taken together, these results provided evidence for the role of neurotrophins in promoting early embryonic development as well as in the growth of granulosa cells by the co-culture system, indicating that BDNF can directly or indirectly promote bovine early embryo development.

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Section: Discussionmentioning

confidence: 99%

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Zhou

Shi

et al. 2008

Animal

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“…11 KGF is synthesized by several mesenchymal cells, such as fibroblasts, 12,15 For KGF to exert its effects, which is mainly in a paracrine manner, KGF needs to bind to its receptor FGFR2-IIIb, which is expressed in keratinocytes and hair follicles. 13,[22][23][24] Activating the KGF receptor leads, through phosphorylation, to proliferation of epithelial cells and is responsible for the morphogenesis of the skin and thus plays an important role during wound healing. 12 After wounding, KGF protein and mRNA concentration increases and they are mainly present in the dermal fibroblasts below the wound and at the wound edges, whereas KGF receptor transcripts and also the corresponding protein were exclusively detected in keratinocytes of the epidermis and the hair follicles.…”

Section: Discussionmentioning

confidence: 99%

“…From previous experiments, we found that KGF, a 26-28 kDa protein, is an epithelial growth factor that exerts its effects mainly in a paracrine manner and is essential for skin morphogenesis. 12,13 KGF has been shown to stimulate epithelial cell differentiation and it appears to control the proliferative-differentiative program from basal to suprabasal cells in the skin. 14 Moreover, KGF stimulates proliferation and exerts an antiapoptotic effect for epithelial cells, which are of major importance for the post-traumatic wound healing.…”

Section: Introductionmentioning

confidence: 99%

Liposomal gene transfer of multiple genes is more effective than gene transfer of a single gene

Jeschke

Klein

2004

Gene Ther

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“…The keratinocyte growth factor, a member of the fibroblast growth factor family stimulates the proliferation of epithelial cells in vitro and also regulates the proliferation and differentiation of a variety of epithelial tissues in vivo [14,15,16]. In the pancreas, previous works have shown the effect of KGF in ductal-cell proliferation [5].…”

Section: Effect Of Kgf On Beta Cell and Ductal Cell Proliferationmentioning

confidence: 99%

Keratinocyte growth factor and beta-cell differentiation in human fetal pancreatic endocrine precursor cells

et al. 2003

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Aims and hypothesis. Keratinocyte growth factor (KGF) is a member of the heparin-binding fibroblast growth factor family with a high degree of specificity for epithelial cells in vitro and in vivo. Our aim was to study the effect of KGF on beta-cell growth and differentiation on islet-like cell clusters derived from human fetal pancreas. Methods. We investigated the effects of KGF, in vitro, on beta-cell differentiation from undifferentiated pancreatic precursor cells and in vivo after transplantating human fetal pancreatic cells into athymic rats treated with KGF. Results. Treatment of islet-like cell clusters with KGF in vitro did not change the number of insulin producing cells, as measured by the measurement of insulin content or DNA. The in vivo treatment of recipient rats with KGF increased the number of beta cells within the grafts 8 weeks after transplantation. At this time, glucose-stimulated insulin secretion was evaluated by glucose stimulation tests in rats bearing the transplants. Measurements of human C-peptide concentrations after glucose challenge showed that the newly differentiated beta cells in the KGF-treated group were functionally competent as opposed to the control group, where the graft failed to release insulin appropriately. Conclusion/interpretation. These findings suggest that in vivo, KGF is capable of inducing human fetal beta-cell expansion. The growth promoting effect of KGF on beta cells occurred mainly through the activation of ductal cell proliferation and their subsequent differentiation into beta cells. [Diabetologia (2003) 46:822-829]

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Human KGF is FGF-Related with Properties of a Paracrine Effector of Epithelial Cell Growth

Cited by 915 publications

References 29 publications

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Liposomal gene transfer of multiple genes is more effective than gene transfer of a single gene

Keratinocyte growth factor and beta-cell differentiation in human fetal pancreatic endocrine precursor cells

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