Human lactoferrin receptor activity in non-encapsulated Haemophilus influenzae

Vogel, L; Geluk, F; Jansen, Henk M.; Dankert, J.; Alphen, Loek van

doi:10.1111/j.1574-6968.1997.tb12723.x

Cited by 8 publications

(8 citation statements)

References 14 publications

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“…For example, some bacterial species are able to bind iron-saturated lactoferrin and use it as a source of iron [42]. Additionally, long-term xylitol consumption can lead to the emergence of xylitol-resistant bacterial populations in humans [41,43].…”

Section: Discussionmentioning

confidence: 99%

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Ammons

Ward²,

Fisher

et al. 2009

International Journal of Antimicrobial Agents

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The medical impact of bacterial biofilms has increased with the recognition of biofilms as a major contributor to chronic wounds such as diabetic foot ulcers, venous leg ulcers and pressure ulcers. Traditional methods of treatment have proven ineffective, therefore this article presents in vitro evidence to support the use of novel antimicrobials in the treatment of Pseudomonas aeruginosa biofilm. An in vitro biofilm model with a clinical isolate of P. aeruginosa was subjected to treatment with either lactoferrin or xylitol alone or in combination. Combined lactoferrin and xylitol treatment disrupted the structure of the P. aeruginosa biofilm and resulted in a >2log reduction in viability. In situ analysis indicated that while xylitol treatment appeared to disrupt the biofilm structure, lactoferrin treatment resulted in a greater than two-fold increase in the number of permeabilised bacterial cells. The findings presented here indicated that combined treatment with lactoferrin and xylitol significantly decreases the viability of established P. aeruginosa biofilms in vitro and that the antimicrobial mechanism of this treatment includes both biofilm structural disruption and permeablisation of bacterial membranes.

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Section: Discussionmentioning

confidence: 99%

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Ammons

Ward²,

Fisher

et al. 2009

International Journal of Antimicrobial Agents

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“…Most iron is stored intracellularly in complexes with proteins such as haem and ferritin, whilst extracellular iron is strongly bound to TF. However, TF-bound iron is also a potential source of iron for extracellular microbes (Hunter et al, 1984;Schryvers and Morris, 1988;Vogel et al, 1997a). Apart from TF, a series of iron-binding proteins, including LF, sequester iron.…”

Section: Innate Immune Responses Limit Iron Availability During Infecmentioning

confidence: 99%

Role of iron in the pathogenesis of respiratory disease

Ali

Kim

Karim

et al. 2017

The International Journal of Biochemistry & Cell Biology

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Iron is essential for many biological processes, however, too much or too little iron can result in a wide variety of pathological consequences, depending on the organ system, tissue or cell type affected. In order to reduce pathogenesis, iron levels are tightly controlled in throughout the body by regulatory systems that control iron absorption, systemic transport and cellular uptake and storage. Altered iron levels and/or dysregulated homeostasis have been associated with several lung diseases, including chronic obstructive pulmonary disease, lung cancer, cystic fibrosis, idiopathic pulmonary fibrosis and asthma. However, the mechanisms that underpin these associations and whether iron plays a key role in the pathogenesis of lung disease are yet to be fully elucidated. Furthermore, in order to survive and replicate, pathogenic micro-organisms have evolved strategies to source host iron, including freeing iron from cells and proteins that store and transport iron. To counter these microbial strategies, mammals have evolved immune-mediated defence mechanisms that reduce iron availability to pathogens. This interplay between iron, infection and immunity has important ramifications for the pathogenesis and management of human respiratory infections and diseases. An increased understanding of the role that iron plays in the pathogenesis of lung disease and respiratory infections may help inform novel therapeutic strategies. Here we review the clinical and experimental evidence that highlights the potential importance of iron in respiratory diseases and infections.

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“…Instead this bacterium produces an array of receptors that selectively bind heme-or iron-containing proteins. Receptors binding hemoglobin and hemoglobin-haptoglobin complexes (5,31,39,43,57), heme-hemopexin (8,70), transferrin (60), and lactoferrin (60,65) have been identified. Because of the importance of iron and heme acquisition to the aerobic growth of H. influenzae, receptors in the outer membrane that selectively bind iron-or heme-containing proteins are potential targets for vaccine development.…”

mentioning

confidence: 99%

“…These include hemoglobin, hemoglobin-haptoglobin complexes, myoglobin, hemopexin-and albumin-bound heme, and transferrin-bound iron of human origin (39,42,55,63). Utilization of iron bound to lactoferrin occurs only in some H. influenzae strains (44,65). H. influenzae does not produce siderophores (44,55,69).…”

mentioning

confidence: 99%

Complex Role of Hemoglobin and Hemoglobin-Haptoglobin Binding Proteins in Haemophilus influenzae Virulence in the Infant Rat Model of Invasive Infection

Seale¹,

Morton²,

Whitby³

et al. 2006

Infect Immun

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Haemophilus influenzae requires an exogenous heme source for aerobic growth in vitro. Hemoglobin or hemoglobin-haptoglobin satisfies this requirement. Heme acquisition from hemoglobin-haptoglobin is mediated by proteins encoded by hgp genes. Both Hgps and additional proteins, including those encoded by the hxu operon, provide independent pathways for hemoglobin utilization. Recently we showed that deletion of the set of three hgp genes from a nontypeable strain (86-028NP) of H. influenzae attenuated virulence in the chinchilla otitis media model of noninvasive disease. The present study was undertaken to investigate the role of the hgp genes in virulence of the wild-type serotype b clinical isolate HI689 in the infant rat model of hematogenous meningitis, an established model of invasive disease requiring aerobic growth. Bacteremia of high titer and long duration (>14 days) and histopathologically confirmed meningitis occurred in >95% of infant rats challenged at 5 days of age with strain HI689. While mutations disrupting either the Hgp-or Hxu-mediated pathway of heme acquisition had no effect on virulence in infant rats, an isogenic mutant deficient for both pathways was unable to sustain bacteremia or produce meningitis. In contrast, mutations disrupting either pathway decreased the limited ability of H. influenzae to initiate and sustain bacteremia in weanling rats. Biochemical and growth studies also indicated that infant rat plasma contains multiple heme sources that change with age. Taken together, these data indicate that both the hgp genes and the hxuC gene are virulence determinants in the rat model of human invasive disease.

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Human lactoferrin receptor activity in non-encapsulated Haemophilus influenzae

Cited by 8 publications

References 14 publications

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Role of iron in the pathogenesis of respiratory disease

Complex Role of Hemoglobin and Hemoglobin-Haptoglobin Binding Proteins in Haemophilus influenzae Virulence in the Infant Rat Model of Invasive Infection

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