Human Leucocyte Antigen A Alleles in The Prediction of Post-Kidney Transplant Lymphoproliferative Disorders, A Single-Center Study

Abstract

Background: Post-transplant lymphoproliferative disorders (PTLPD) are frequently encountered after kidney transplantation. Human leucocyte antigen (HLA)A alleles were shown to be a predictor of development of these disorders. Objective: Study of prediction of human leucocyte antigen HLA-A alleles in the development of PTLPD. Methods: This study included 50 Egyptian kidney transplant (KT) recipients who underwent live-donor kidney transplantation, between 2010 to 2022. HLA-A alleles phenotyping was characterized by sequence specific oligonucleotide probes (SSOP). Nondestructive form of PTLPD was diagnosed by presence of lymphocytosis followed by assessment of, (CD45 and CD 19) mature B lymphocytes and blast cells (CD5 and CD10) with ratio of kappa/lambda by flow cytometry. The association between the development of this form and different HLA-A alleles was studied. Results: Out of fifty recipients, five patients developed a non-destructive form of PTLPD in the median duration of 30 months (ranged, 15-50 months) post transplantation. A significant association was found between the expression of HLA-A01 (P=0.002, OR=1.020), CI (0.996-1.045) and HLA-A02 (P=0.042, OR=1.019), CI (1.001-1.037) and the development of PTLPD. Furthermore, HLA-A02 alleles were found to be a significant corelate with onset of development PTLPD. Conclusion: Among Egyptian kidney transplant recipients, HLA-A01 and A02 alleles are good predictors of development of early non distractive form of PTLPD.