Human Leukocyte Antigen: Class I Allele Frequencies and Haplotype Distributionin a Tertiary Care Hospital in Bangladesh

Abstract

Human leukocyte antigen (HLA) are cell surface glycoproteins encoded by Major Histocompatibility Complex (MHC) gene of human genome. HLA antigen frequency and haplotype distribution are useful for determining disease association, origin, migration and genetic relationship between populations and predicting the outcome of transplantation. Thus, the present study was carried out to identify HLA class I (HLA-A and HLA-B) antigen and haplotype distribution among a selected Bangladeshi population. This retrospective study was conducted among 1070 individuals, who were referred by clinicians for HLA typing at the Tissue Typing Laboratory of the Department of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU) during the period 2009 to 2011. For HLA typing, blood was collected in heparin containing tube and the laboratory tests were performed by the microlymphocytotoxicity technique according to manufacturer's instructions. Out of 19 HLA-A and 37 HLA-B antigens tested, a total of 19/19 and 36/37 antigens were detected respectively in this study. The most frequent antigens of HLA-A and HLA-B detected were A11 (25.4%), A24 (16.6%), B75 (18.1%) and B35 (11.3%). The least antigen frequency detected for HLA-A locus were A69 (0.09%), A26 (0.28%), A34 (0.28%), while for HLA-B locus were B81 (0.09%) and B56 (0.09%). Among the HLA-A and HLA-B antigens, some alleles were found to be homozygote such as A11 (4.0%), A2 (2.7%), A24 (2.1%) and B75 (2.4%), B35 (1.8%), and B44 (1.4%) respectively. The most frequent haplotype in the study population were A11: B75 (4.9%). The most frequent antigens of HLA-A and HLA-B detected were A11 (25.4%), B75 (18.1%) respectively. The distribution of HLA haplotypes among the study population indicates that it has the influence of Oriental and Asian populations. Thus, this study will be helpful to provide valuable information for population genetics and HLA disease association analysis.

HLA gene polymorphism among Bangladeshi patients with end-stage renal disease awaiting kidney transplantation

“…In this study, among ESRD patients and donors, A, B and DRB1 locus were found to be similar and both groups were in genetic equilibrium found to be in agreement within both groups. Additionally, these highest frequent alleles were also be reported highest from a previous study, 10 as well as from another population based study from Bangladesh. 11 As cadaveric transplant has not yet started in Bangladesh, most of the donors of this study were blood relatives of the patients.…”