Human leukocyte antigen-G polymorphism in relation to expression, function, and disease

Larsen, Margit Hørup; Hviid, Thomas Vauvert F.

doi:10.1016/j.humimm.2009.07.015

Cited by 98 publications

(93 citation statements)

References 98 publications

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“…The capability of HLA-G1 and HLA-G5 to form multimers has been demonstrated both in vivo and in vitro (24,34 ). HLA-G has a low polymorphism rate, and most of the alleles do not alter the amino acid sequence, do not change the secondary structure of the heavy chains, and do not produce high molecular weight isoforms (35 ). However, those polymorphisms that alter the epitopes recognized by the antibodies could affect quantification based on the binding to these antibodies, but not the size of the band detected in the Western blot.…”

Section: Soluble Hla-g-like Molecules In Exudatesmentioning

confidence: 99%

Identification of Circulating Nonclassic Human Leukocyte Antigen G (HLA-G)–Like Molecules in Exudates

et al. 2011

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BACKGROUND HLA-G in biological fluids has been proposed to be useful as a tumor marker as both a diagnostic and prognostic factor. Most HLA-G measurement procedures are based on ELISA methods using highly specific antibodies. However, results of published studies are in conflict regarding the clinical utility and even the nature of HLA-G present in circulation. METHODS We collected 118 exudates, 94 from cancer patients and 24 from patients without tumors. We measured HLA-G concentrations by ELISA using MEM-G/9 or G233 as capture antibody. Samples were immunoprecipitated with an anti–HLA-G antibody and analyzed by Western blot using a different anti–HLA-G antibody. RESULTS Discrepancies in HLA-G concentrations in exudates were observed depending on what capture anti–HLA-G antibody was used for ELISA (r = 0.376). These discrepancies were not observed when the ELISAs were performed using culture supernatants from HLA-G1–transfected cells (r = 0.983). Immunoprecipitation and Western blot of cell culture supernatants with 2 different anti–HLA-G antibodies produced the typical band at 39 kDa assigned to HLA-G. When the immunoprecipitation and western blot were performed with exudates, however, there were bands at 53 kDa and 70–76 kDa, higher molecular weights than those usually assigned to HLA-G. These HLA-G–like molecules were associated with β2-microglobulin and could also form disulfide bridges with other HLA-G–like molecules. CONCLUSIONS The main HLA-G antigenic molecules in exudates are HLA-G–like complexes, a factor that should be considered when analyzing HLA-G in biological fluids.

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Section: Soluble Hla-g-like Molecules In Exudatesmentioning

confidence: 99%

Identification of Circulating Nonclassic Human Leukocyte Antigen G (HLA-G)–Like Molecules in Exudates

et al. 2011

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“…However, the mRNA stability conferred by the 14-bp insertion may exert a negative effect on HLA-G expression, and the 14-bp insertion is associated with lower soluble HLA-G levels. 15 The 14-bp insertion also is associated with the generation of a more stable mRNA, but these transcripts have minimal effects on overall HLA-G expression. Conversely, decreased protein expression is associated with the insertion allele.…”

Section: Discussionmentioning

confidence: 99%

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2014

Exp Clin Transplant

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Objectives: The human leukocyte antigen-G may have a positive role in graft acceptance in human organ transplant. Several studies have reported an association between the human leukocyte antigen-G-14-base-pair-insertion/deletion polymorphism and risk of developing kidney graft rejection, but the results are inconclusive. We performed a metaanalysis to evaluate this association. Conclusions: There may be no association between 14-base-pair polymorphisms and risk of developing kidney allograft rejection. Additional studies with larger sample size and better study design are justified.

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“…The other null allele -HLA-G*01:13N -contains a point mutation (C to T) at codon 54 in exon 2 (a1 domain) that causes a premature stop codon (TAG) leading to no HLA-G isoform production. The majority of polymorphisms found in the coding regions were seen in codons 31, 35, 57 and 69 of exon 2 and in codons 93, 107 and 110 of exon 3 [63]. Frequencies of the different HLA-G alleles vary between ethnic populations.…”

Section: Functional Impact Of Hla-g Polymorphismmentioning

confidence: 99%

Role of HLA-G and other immune mechanisms in pregnancy

et al. 2013

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Pregnancy loss (abortion) and pre-eclampsia represent the most common disorders in pregnant women. Besides infection, there are anatomical, endocrinological, genetic and immunological factors that can induce pregnancy disorders. Because the exact mechanisms of physiological pregnancy maintenance are still not clearly understood, the search for genes and proteins fulfilling this role is still in progress. One of the immune molecules that plays a beneficial role in pregnancy is the nonclassical HLA-G molecule. The molecule is mainly expressed on trophoblast cells in the foetal placenta and induces the immune tolerance of the foetus via its interaction with inhibitory receptors on maternal NK cells and CD8+ T lymphocytes. In relation to pregnancy disorders, associations between HLA-G polymorphism, HLA-G level and HLA-G function were described. Thus, the HLA-G molecule can be used as a new diagnostic marker and, potentially, for the future therapy of pregnancy disorders.

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Human leukocyte antigen-G polymorphism in relation to expression, function, and disease

Cited by 98 publications

References 98 publications

Identification of Circulating Nonclassic Human Leukocyte Antigen G (HLA-G)–Like Molecules in Exudates

Identification of Circulating Nonclassic Human Leukocyte Antigen G (HLA-G)–Like Molecules in Exudates

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Role of HLA-G and other immune mechanisms in pregnancy

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