Human Leukocyte Antigens I and II Haplotypes Associated With Human Papillomavirus 16-Positive Invasive Cervical Cancer in Mexican Women

Abstract

Infection with human papillomavirus (HPV), mainly HPV type 16, is the major etiologic factor associated with cervical cancer (CC), but HPV infection alone is not sufficient for progression of precursor lesions. Host genetic susceptibility may lead to abnormal immune response resulting from virus persistence. Several studies have suggested a possible association with specific human leukocyte antigen (HLA) class I and II alleles and CC, but results are not consistent. The association of genetic HLA class I (A and B) and HLA class II (DR*B1 and DQ*B1) haplotypes with HPV16-positive CC (n = 104) and base population controls (n = 104) was evaluated in this Mexican population study. Sequence-specific primer HLA genes were determined by polymerase chain reaction (PCR)-based methods in peripheral blood cell counts (PCR sequence-specific oligonucleotides). The cervical swabs of 208 women were tested for HPV16 by Hybrid Capture II. Allele and haplotype HLA frequencies, Hardy-Weinberg tests, and a haplotype homogeneity test were estimated using the Arlequin software v. 3.01. Odds ratio (OR) was calculated to compare cases and control women. Consistent associations across other studies in women with CC and infected by HPV16 were observed for HLA-DRB1*15 (OR, 3.9; 95% CI, 1.6-10.2) and the haplotype DRB1*15 DQB1*0602 (OR, 4.1; 95% CI, 1.4-12.7) compared with control women. The HLA-A2-B44-DR4-DQ*0302, HLA-A24-B35-DR16-DQ*0301, and HLA-A2-B40-DR4-DQ*0302 haplotypes showed a positive association with CC (OR, >1), whereas HLA-A2-B39-DR4-DQ*0302, HLA-A24-B35-DR4-DQ*0302, and HLA-A68-B40-DR4-DQ*0302 showed a negative association (OR, <1). These results support the hypothesis that some HLA class I and II haplotypes could be involved with susceptibility for developing CC.

Analysis of HLA-DQB1 allele polymorphisms in Uyghur women with cervical cancer

“…As a component of the body's immunological and genetic composition, HLA plays an important role in the occurrence and development of cervical cancer (Mehta et al, 2008;de Araujo et al, 2009). Studies from various countries have demonstrated that the frequency of certain HLA alleles increases in cervical cancer cases in some ethnicities and may correlate with genetic susceptibility (de Araujo et al, 2008;Hernández-Hernández et al, 2009;Kohaar et al, 2009). In contrast, the reduced frequency of certain alleles may prevent the development of cervical cancer (Castro et al, 2007;Eiguchi et al, 2008).…”

Pediatric adrenocortical tumors: morphological diagnostic criteria and immunohistochemical expression of matrix metalloproteinase type 2 and human leucocyte-associated antigen (HLA) class II antigens

“…It is not yet known when these antigens appear in the adrenal cortex, but they are probably involved in cellular differentiation. Loss of expression of HLA class antigens or deregulation has been reported in a great variety of tumors, including breast, cervix, larynx, colon, and pancreas cancers [26][27][28][29][30][31][32]. In a study of gene expression by microarray analysis, carried out on 24 pediatric ACTs (5 adenomas, 18 carcinomas, 1 tumor classified as indeterminate for malignancy), West et al [12] identified 52 probe sets for which significant differences were found when comparing adrenocortical adenomas versus carcinomas.…”

Prevalence of Human Papillomavirus in Women from the State of Michoacan, Mexico, Showed High Frequency of Unusual Virus Genotypes

“…This may be related to the major histocompatibility complex (MHC) [27][28][29] and health of the patient, the homeostasis of the immune system, or the historical evolution of the infection, which we did not explore in this study.…”