Human-like performance umami electrochemical biosensor by utilizing co-electrodeposition of ligand binding domain T1R1-VFT and Prussian blue

Research progress in the screening and evaluation of umami peptides

“…(2019) developed an electrochemical biosensor based on T1R1 to evaluate the interaction between four umami ligands (i.e., GMP, MSG, IMP, and WSA) and umami receptors. Because of the complexity of the umami receptor structure, a biosensor based on the T1R1 umami ligand‐binding domain VFT was developed to simplify the sensor construction process (Ahn et al., 2018; Li et al., 2021).…”

“…The type, concentration, processing conditions, and hydrolysis will affect the umami intensity of enzyme hydrolytic products and polypeptides (Su et al., 2011). The same protein can be treated under different conditions to produce polypeptides with different flavors (Bruno et al., 2019; Li et al., 2021; Wei et al., 2018). To select suitable enzymes and protein substrates, it is generally necessary to conduct multiple groups of parallel experiments to screen different enzyme types, dosage, and reaction conditions to obtain the hydrolysis products with the most umami.…”

A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H2O2 system

“…Electrochemiluminescence (ECL) analysis is a novel and powerful method in the sensing field, which combines the merits of both chemiluminescence and electrochemical methods. 19,20 Aptamers with excellent stability and easy storage provide a possibility for the assembly of sensors and a new choice for the chemiluminescence method. In recent years, researchers have constructed a series of electrochemiluminescence aptasensors that combine the molecular recognition function of aptamers and the signal indication function of electrochemiluminescence.…”

Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample

“…To address all these issues related to conventional and advanced techniques, biosensors have been developed ( Aziz et al, 2022 ). The development of biosensors can solve the abovementioned problems ( Asif et al, 2019 ; Aziz et al, 2019b ), such as colorimetry ( Yao et al, 2020 ), fluorescence ( Shi et al, 2015 ), and electrochemistry ( Li et al, 2021 ). Among them, the electrochemical method has received widespread attention because of the low cost, easy handling, and portability ( Asif et al, 2022 ).…”

“…At the same time, the introduction of carboxylated single-walled carbon nanotubes (C-SWCNTs) can further improve the specific surface area and the electrical conductivity of PI-5-CA. Due to its tubular hollow structure, carbon nanotubes have unique electrical conductivity, high strength, flexibility, stable chemical properties, and excellent specific surface area ( Kumar and Sundramoorthy, 2019 ; Li et al, 2021 ). Through chemical synthesis, PI-5-CA and C-SWCNTs are synthesized into a composite material to syndicate the electrochemical advantages of the two, and using their abundant carboxyl functional groups to combine with various biological recognition molecules ( Yang et al, 2019 ).…”