Human &lt;em&gt;Vastus Lateralis&lt;/em&gt; Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Baczynska, Alicja M.; Shaw, Sarah; Roberts, Helen; Cooper, Cyrus; Sayer, Avan Aihie; Patel, Harnish P.

doi:10.3791/53075

Cited by 11 publications

(12 citation statements)

References 22 publications

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“…Initial geometry for all models was built from a single human muscle fiber cross-section obtained from the literature , where this group undertook antibody staining of muscle biopsies from four young healthy male human participants. The vastus lateralis is a frequently used muscle in biopsy studies since it is a large, easily accessed muscle (Baczynska et al, 2016). In this case, we used a histological image from human vastus lateralis as a representative of fiber bundle architecture for human lower limb muscle.…”

Section: Methodsmentioning

confidence: 99%

A Coupled Mechanobiological Model of Muscle Regeneration In Cerebral Palsy

Khuu

Fernandez

Handsfield

2021

Front. Bioeng. Biotechnol.

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Cerebral palsy is a neuromusculoskeletal disorder associated with muscle weakness, altered muscle architecture, and progressive musculoskeletal symptoms that worsen with age. Pathological changes at the level of the whole muscle have been shown; however, it is unclear why this progression of muscle impairment occurs at the cellular level. The process of muscle regeneration is complex, and the interactions between cells in the muscle milieu should be considered in the context of cerebral palsy. In this work, we built a coupled mechanobiological model of muscle damage and regeneration to explore the process of muscle regeneration in typical and cerebral palsy conditions, and whether a reduced number of satellite cells in the cerebral palsy muscle environment could cause the muscle regeneration cycle to lead to progressive degeneration of muscle. The coupled model consisted of a finite element model of a muscle fiber bundle undergoing eccentric contraction, and an agent-based model of muscle regeneration incorporating satellite cells, inflammatory cells, muscle fibers, extracellular matrix, fibroblasts, and secreted cytokines. Our coupled model simulated damage from eccentric contraction followed by 28 days of regeneration within the muscle. We simulated cyclic damage and regeneration for both cerebral palsy and typically developing muscle milieus. Here we show the nonlinear effects of altered satellite cell numbers on muscle regeneration, where muscle repair is relatively insensitive to satellite cell concentration above a threshold, but relatively sensitive below that threshold. With the coupled model, we show that the fiber bundle geometry undergoes atrophy and fibrosis with too few satellite cells and excess extracellular matrix, representative of the progression of cerebral palsy in muscle. This work uses in silico modeling to demonstrate how muscle degeneration in cerebral palsy may arise from the process of cellular regeneration and a reduced number of satellite cells.

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Section: Methodsmentioning

confidence: 99%

A Coupled Mechanobiological Model of Muscle Regeneration In Cerebral Palsy

Khuu

Fernandez

Handsfield

2021

Front. Bioeng. Biotechnol.

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“…Aortic pulse wave velocity (PWV) was then measured in triplicate using a SphygmoCor (AtCor Medical, Sydney, Australia) . A resting muscle biopsy was then taken from the lateral portion of the m. vastus lateralis under local anaesthesia (0.5% marcaine), using the Weil-Blakesley conchotome technique (Baczynska et al 2016). Finally, insulin sensitivity was measured using an oral glucose tolerance test (OGTT) (Matsuda & DeFronzo, 1999).…”

Section: Experimental Protocolmentioning

confidence: 99%

Home‐hit improves muscle capillarisation and eNOS/NAD(P)Hoxidase protein ratio in obese individuals with elevated cardiovascular disease risk

Scott

Shepherd

Hopkins

et al. 2019

The Journal of Physiology

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Key points Obesity and sedentary behaviour are associated with capillary rarefaction and impaired muscle microvascular vasoreactivity, due to reduced nitric oxide bioavailability. Low‐volume high‐intensity interval training (HIT) is a time‐efficient alternative to traditional moderate‐intensity continuous training (MICT), but its effect on the muscle microvasculature has not been studied. The applicability of current laboratory‐ and gym‐based HIT protocols for obese individuals with low fitness and mobility has been disputed by public health experts, who cite the strenuous nature and complex protocols as major barriers. Therefore, we developed a virtually supervised HIT protocol targeting this group that can be performed at home without equipment (Home‐HIT). This study is the first to show that 12 weeks of virtually supervised Home‐HIT in obese individuals with elevated cardiovascular disease risk leads to similar increases in capillarisation and eNOS/NAD(P)Hoxidase protein ratio within the muscle microvascular endothelium as virtually supervised home‐based MICT and laboratory‐based HIT, while reducing many of the major barriers to exercise. Abstract This study investigated the effect of a novel virtually supervised home‐based high‐intensity interval training (HIT) (Home‐HIT) intervention in obese individuals with elevated cardiovascular disease (CVD) risk on capillarisation and muscle microvascular eNOS/NAD(P)Hoxidase ratio. Thirty‐two adults with elevated CVD risk (age 36 ± 10 years; body mass index 34.3 ± 5 kg m−2; V̇O2 peak 24.6 ± 5.7 ml kg min−1), completed one of three 12‐week training programmes: Home‐HIT (n = 9), laboratory‐based supervised HIT (Lab‐HIT; n = 10) or virtually supervised home‐based moderate‐intensity continuous training (Home‐MICT; n = 13). Muscle biopsies were taken before and after training to assess changes in vascular enzymes, capillarisation, mitochondrial density, intramuscular triglyceride content and GLUT4 protein expression using quantitative immunofluorescence microscopy. Training increased V̇O2 peak (P < 0.001), whole‐body insulin sensitivity (P = 0.033) and flow‐mediated dilatation (P < 0.001), while aortic pulse wave velocity decreased (P < 0.001) in all three groups. Immunofluorescence microscopy revealed comparable increases in total eNOS content in terminal arterioles and capillaries (P < 0.001) in the three conditions. There was no change in eNOS ser1177 phosphorylation (arterioles P = 0.802; capillaries P = 0.311), but eNOS ser1177/eNOS content ratio decreased significantly following training in arterioles and capillaries (P < 0.001). Training decreased NOX2 content (arterioles P < 0.001; capillaries P < 0.001), but there was no change in p47phox content (arterioles P = 0.101; capillaries P = 0.345). All measures of capillarisation increased (P < 0.05). There were no between‐group differences. Despite having no direct supervision during exercise, virtually supervised Home‐HIT resulted in comparable structural and endothelial enzymatic changes in the skeletal muscle mic...

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“…We measured grip strength (kg) by taking three assessments from each hand using a Jamar dynamometer, with the maximum value used in analyses [22]. We recorded the time take to complete five chair stands [23]. We tested physical performance by measuring normal gait speed over 4 metres and tests of standing balance.…”

Section: Methodsmentioning

confidence: 99%

Advancing our understanding of skeletal muscle across the lifecourse: protocol for the MASS_Lifecourse study and characteristics of the first 80 participants

Dodds

Hurst

Hillman

et al. 2021

Preprint

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Introduction Sarcopenia, the age-related loss of skeletal muscle strength and mass, carries a significant burden for affected individuals. There has been little investigation of sarcopenia using experimental medicine techniques to study human muscle tissue in detail. The aim of the Muscle Ageing Sarcopenia Studies Lifecourse (MASS_Lifecourse) study is to recruit up to 160 participants, equally divided between females and males between ages 45 and 85 years for detailed phenotyping of skeletal muscle health. Here we describe the protocol for the study and the characteristics of the first 80 participants. Methods We are recruiting participants from three sources in the north-east of England. Study fieldwork comprises a home visit (or videocall) for consent and assessment of health, cognition, lifestyle, and wellbeing. This is followed by a visit to a clinical research facility for assessment of sarcopenia status and collection of samples including a vastus lateralis muscle biopsy. We produced descriptive statistics for the first 80 participants, including expressing their grip strength relative to normative data in the form of Z-scores. Results The first 80 participants (53.8% female) covered the target ages, ranging from 48 to 84 years. They were regularly physically active, reported good physical function and had a prevalence sarcopenia (including probable sarcopenia) of 11.3% based on the revised European consensus. Their grip strength was similar to that in the general population, with a mean Z-score of 0.09 standard deviations (95% CI: -1.64, 1.83) above that expected. Conclusions The MASS_Lifecourse study combines comprehensive health and lifestyle data with a range of biological samples including skeletal muscle. The findings from planned analyses should contribute to improvements in the diagnosis, treatment and prevention of sarcopenia.

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Human <em>Vastus Lateralis</em> Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Cited by 11 publications

References 22 publications

A Coupled Mechanobiological Model of Muscle Regeneration In Cerebral Palsy

A Coupled Mechanobiological Model of Muscle Regeneration In Cerebral Palsy

Home‐hit improves muscle capillarisation and eNOS/NAD(P)Hoxidase protein ratio in obese individuals with elevated cardiovascular disease risk

Advancing our understanding of skeletal muscle across the lifecourse: protocol for the MASS_Lifecourse study and characteristics of the first 80 participants

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