Human lung cell growth is not stimulated by lead ions after lead chromate-induced genotoxicity

Wise, Sandra S.; Holmes, Amie L.; Moreland, Jonathan A.; Xie, Hong; Sandwick, Sarah J.; Stackpole, Megan M.; Fomchenko, Elena; Teufack, Sonia; May, Alfred; Katsfis, Spiros P.; Wise, John Pierce

doi:10.1007/s11010-005-8217-0

Cited by 15 publications

(18 citation statements)

References 27 publications

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“…But, it does raise a question about the possible role of lead in the toxicity of the compound. Our previous studies in human cells demonstrated that lead does not contribute to the cytotoxic and genotoxic effects of lead chromate because it is poorly absorbed by cells (Wise, S. et al, 2004; 2005). We did check sea turtle cells and found very low lead uptake after lead chromate exposure (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…Lead chromate (CAS# 7758-97-6, ACS reagent minimum 98% purity), a particulate Cr(VI) compound was administered as a suspension in water as previously described (Wise J et al, 2002). Lead chromate does not fully dissolve in tissue culture while sodium chromate does (Holmes et al, 2005; Wise S. et al, 2005). Thus, direct comparisons of the two chemicals using a common unit of measure is difficult.…”

Section: Methodsmentioning

confidence: 99%

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Hexavalent chromium is cytotoxic and genotoxic to hawksbill sea turtle cells

Wise

Xie

Fukuda

et al. 2014

Toxicology and Applied Pharmacology

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Sea turtles are a charismatic and ancient ocean species and can serve as key indicators for ocean ecosystems, including coral reefs and sea grass beds as well as coastal beaches. Genotoxicity studies in the species are absent, limiting our understanding of the impact of environmental toxicants on sea turtles. Hexavalent chromium (Cr(VI)) is a ubiquitous environmental problem worldwide, and recent studies show it is a global marine pollutant of concern. Thus, we evaluated the cytotoxicity and genotoxicity of soluble and particulate Cr(VI) in hawksbill sea turtle cells. Particulate Cr(VI) was both cytotoxic and genotoxic to sea turtle cells. Concentrations of 0.1, 0.5, 1, and 5 μg/cm2 lead chromate induced 108, 79, 54, and 7 percent relative survival, respectively. Additionally, concentrations of 0, 0.1, 0.5, 1, and 5 μg/cm2 lead chromate induced damage in 4, 10, 15, 26, and 36 percent of cells and caused 4, 11, 17, 30, and 56 chromosome aberrations in 100 metaphases, respectively. For soluble Cr, concentrations of 0.25, 0.5, 1, 2.5, and 5 μM sodium chromate induced 84, 69, 46, 25, and 3 percent relative survival, respectively. Sodium chromate induced 3, 9, 9, 14, 21, and 29 percent of metaphases with damage, and caused 3, 10, 10, 16, 26, and 39 damaged chromosomes in 100 metaphases at concentrations of 0, 0.25, 0.5, 1, 2.5, and 5 μM sodium chromate, respectively. These data suggest that Cr(VI) may be a concern for hawksbill sea turtles and sea turtles in general.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Hexavalent chromium is cytotoxic and genotoxic to hawksbill sea turtle cells

Wise

Xie

Fukuda

et al. 2014

Toxicology and Applied Pharmacology

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“…It is established that lead chromate partially dissolves outside the cell, releasing Pb and Cr ions that then enter the cell (9). Once inside the cell, the Cr ions induce the cytotoxic, growth-inhibiting, and genotoxic effects observed after exposure to lead chromate and the lead ions have no apparent effect (14,15,17,18). Thus far, the potential effects of Cr ions on centrosome amplification have not been studied, but a 30-hour exposure to soluble chromate induced aneuploidy, which suggests that Cr ions could have the capacity to cause centrosome amplification, although that study did not consider it (31).…”

Section: Discussionmentioning

confidence: 99%

“…The internalized Cr ions induce chromosome aberrations, DNA adducts, and DNA double-strand breaks (10)(11)(12)(13)(14)(15), whereas the internalized Pb ions are generally nongenotoxic (8,16). Two studies investigating potential epigenetic effects of Pb in lead chromate-induced carcinogenesis also show that Pb does not interfere with Crinduced cell death (17) or cause mitotic stimulation of Cr-damaged cells (18). In addition, internalized particles seem to have no toxic effect except for possibly a small contribution to cytotoxicity (17).…”

Section: Introductionmentioning

confidence: 99%

Chronic Exposure to Lead Chromate Causes Centrosome Abnormalities and Aneuploidy in Human Lung Cells

et al. 2006

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Hexavalent chromium [Cr(VI)] compounds are established human lung carcinogens. The carcinogenicity of Cr(VI) is related to its solubility, with the most potent carcinogens being the insoluble particulate Cr(VI) compounds. However, it remains unknown why particulate Cr(VI) is more carcinogenic than soluble Cr(VI). One possible explanation is that particulates may provide more chronic exposures to chromate over time. We found that aneuploid cells increased in a concentration-and time-dependent manner after chronic exposure to lead chromate. Specifically, a 24-hour lead chromate exposure induced no aneugenic effect, whereas a 120-hour exposure to 0.5 and 1 Mg/cm 2 lead chromate induced 55% and 60% aneuploid metaphases, respectively. We also found that many of these aneuploid cells were able to continue to grow and form colonies. Centrosome defects are known to induce aneuploidy; therefore, we investigated the effects of chronic lead chromate exposure on centrosomes. We found that centrosome amplification in interphase and mitotic cells increased in a concentration-and time-dependent manner with 0.5 and 1 Mg/cm 2 lead chromate for 120 hours, inducing aberrant centrosomes in 18% and 21% of interphase cells and 32% and 69% of mitotic cells, respectively; however, lead oxide did not induce centrosome amplification in interphase or mitotic cells. There was also an increase in aberrant mitosis after chronic exposure to lead chromate with the emergence of disorganized anaphase and mitotic catastrophe. These data suggest that one possible mechanism for lead chromate-induced carcinogenesis is through centrosome dysfunction, leading to the induction of aneuploidy. (Cancer Res 2006; 66(8): 4041-8)

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“…Chromium is a recognized carcinogen of the respiratory tract, lungs, and gastric mucosa epithelium [50][51][52][53][54][55][56]. Higher levels of Cr were found in blood plasma of people suffering from lymphoproliferative diseases [57], as well as hexavalent Cr genotoxicity measured by the amount of chromosome aberrations [58,59] and the amount of single and/or double-strand DNA breakage in human lymphocytes in vitro [60,61] and animal cells [62,63].…”

Section: Correlation Coefficients Between Elements In Rainwater Andmentioning

confidence: 99%

The Content of Elements in Rainwater and Its Relation to the Frequency of Hospitalization for Chronic Lymphocytic Leukemia and Chronic Myeloid Leukemia in Opole Voivodship, Poland, During 2000–2002

Szyguła

Bunio

Tubek

2010

Biol Trace Elem Res

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Environmental factors play an essential role in the etiology of diseases of the hematopoietic system. Such factors include soil and water pollution and the presence of metals and toxic compounds in the air. Measuring the content of metallic elements in rainwater has become an accepted procedure for environmental pollution monitoring. In accordance with the above, it was decided to study relations between the content of selected elements in rainwater and hospitalization frequency due to chronic lymphocytic leukemia (CLL, C91 on ICD-10) and chronic myeloid leukemia (CML, C92 on ICD-10). It can be assumed that hospitalization frequency is a reliable indicator of exacerbations of these diseases. The annual average of hospitalizations due to a given disease during the years 2000-2002 was correlated with the annual average content of a given element in rainwater using the Spearman's correlation indicator to describe the relationship between the element content and the disease that is possibly a consequence of the element's presence in rainwater. In cases of CLL for all the subjected population and for men, no statistically significant correlations were found. For women, statistically significant correlations were found for chromium (r = 0.66), lead (r = 0.58), copper (r = 0.58), and cadmium (r = 0.51). For CML in all the studied population significant, negative correlations were found for magnesium (r = -0.6) and zinc (r = -0.52). In men, significant negative correlations were seen for magnesium (r = -0.69 and zinc (r = -0.55). No significant correlations were found in women. These results indicate the need of taking into account the environmental and gender factors in research connected with these diseases, which can be probably of help in improvements of therapy efficiency.

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Human lung cell growth is not stimulated by lead ions after lead chromate-induced genotoxicity

Cited by 15 publications

References 27 publications

Hexavalent chromium is cytotoxic and genotoxic to hawksbill sea turtle cells

Hexavalent chromium is cytotoxic and genotoxic to hawksbill sea turtle cells

Chronic Exposure to Lead Chromate Causes Centrosome Abnormalities and Aneuploidy in Human Lung Cells

The Content of Elements in Rainwater and Its Relation to the Frequency of Hospitalization for Chronic Lymphocytic Leukemia and Chronic Myeloid Leukemia in Opole Voivodship, Poland, During 2000–2002

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