Human mast cell migration in response to members of the transforming growth factor-β family

Olsson, Niclas; Piek, Ester; Dijke, Peter ten; Nilsson, Gunnar

doi:10.1002/jlb.67.3.350

Cited by 113 publications

(101 citation statements)

References 35 publications

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“…These effects of TGF-β on function of MCs depend on the concentration of TGF-β. [7] This behavior illustrates the autocrine property of MCs. [8] In addition, there is dearth in the literature regarding TGF-β/MCs relation; though it is believed to be chemotactic for endothelial cells and also causes growth of fibrous tissue.…”

Section: Role Of Mcs In Ofsmentioning

confidence: 82%

“…[6] They are derived from the bone marrow and are mobile. [7] MCs exit the bone marrow in an immature form and mature at the tissue site. [13] They can be easily identified using toluidine blue stain due to metachromasia exhibited by the granules.…”

Section: Role Of Mcs In Ofsmentioning

confidence: 99%

“…MCs have serine/threonine types I and II receptors for TGF-β, showing that TGF-β responses are elicited via signaling through TβR-I/TβR-II complexes. These membrane receptors cause signal transduction, [7] and hence, it can be deducted that TGF-β influences MC activity. TGF-β can impede functioning of MC, [6] or acts as a chemoattractant for MCs, [7] depending on its concentration.…”

Section: Role Of Mcs In Ofsmentioning

confidence: 99%

“…These membrane receptors cause signal transduction, [7] and hence, it can be deducted that TGF-β influences MC activity. TGF-β can impede functioning of MC, [6] or acts as a chemoattractant for MCs, [7] depending on its concentration. These effects of TGF-β on function of MCs depend on the concentration of TGF-β.…”

Section: Role Of Mcs In Ofsmentioning

confidence: 99%

“…While TGF-β inhibited MCs in some studies, few others have shown it to be a chemotaxin for MCs. [6,7] Furthermore, there is scarcity in the literature about the interaction TGF-β with MCs in OSF. Henceforward, this attempt was made to connect TGF-β expression and MC in different OSF grades, so as to further comprehend the pathogenesis of this disease and thereby open new avenues for its management.…”

Section: Introductionmentioning

confidence: 99%

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Correlation between transforming growth factor-beta expression and mast cell count in different grades of oral submucous fibrosis

Kumar¹,

Suma²,

Kumar³

et al. 2016

JCRI

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Background and Objectives:In India, oral submucous fibrosis (OSF) is a commonly encountered precancerous disorder. It is a disease with a protracted course and characteristic clinical features caused by the habit of consuming areca nut. Histologically, the main pathology is extensive fibrosis of the mucosa. OSF can be categorized into four grades based on histology. Inflammatory cells and keratocytes are stimulated by the areca nut alkaloids, and they release transforming growth factor-beta (TGF-β). It has been advocated to play a major role in OSF pathogenesis by increasing collagen synthesis and preventing collagen degradation. Mast cells (MCs) are the additional key players in the pathogenesis of OSF. There is confusion regarding the functional relationship of MC with TGF-β. Few studies have shown TGF-β can repress MC function, whereas other studies have established that TGF-β is a chemotaxin for MCs. However, there is dearth in literature regarding their interaction in OSF. Hence, this study attempted to show a relationship between TGF-β and MC in different grades of the disease, so as to further explicate the pathogenesis of OSF. Materials and Methods: A total of 48 OSF cases were stained using TGF-β antibody. Its staining intensity and staining area were calculated. Sections from same blocks were also stain MCs using 1% toluidine blue. The number of MCs was counted for all the cases. Expression of TGF-β was correlated with MC count in all OSF grades. Results: On correlating TGF-β and MC between the histologic grades, there was a strong negative correlation for Grade I, moderately positive for Grade II and no correlation for Grade III OSF. Interpretation and Conclusions: Among the different grades of OSF, the correlation between MC and TGF-β varies, depending on the severity of inflammation, and the concentration of TGF-β and further investigation in this field may elucidate their role in determining the severity of OSF.

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Section: Role Of Mcs In Ofsmentioning

confidence: 82%

Section: Role Of Mcs In Ofsmentioning

confidence: 99%

Section: Role Of Mcs In Ofsmentioning

confidence: 99%

Section: Role Of Mcs In Ofsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Correlation between transforming growth factor-beta expression and mast cell count in different grades of oral submucous fibrosis

Kumar¹,

Suma²,

Kumar³

et al. 2016

JCRI

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The chemokine receptor CXCR4 is expressed within the mast cell lineage and its ligand stromal cell-derived factor-1α acts as a mast cell chemotaxin

et al. 2000

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In this study we provide evidence that the chemokine stromal cell‐derived factor‐1α (SDF‐1α) acts as a mast cell chemoattractant through interactions with its receptor CXCR4 expressed on mast cell progenitors in the blood as well as on in vitro‐developed and leukemic mast cells. We found expression of CXCR4 on cord blood‐derived mast cells (CBMC) and on the human mast cell line HMC‐1, analyzed by RNAse protection assay and flow cytometry. SDF‐1α induced intracellular calcium mobilization in HMC‐1 cells and was chemotactic for both HMC‐1 cells and CBMC. The activity of SDF‐1α was completely blocked by treating the cells with pertussis toxin, indicating the involvement of Gi‐proteins in the signaling. By applying a transwell assay we could show that SDF‐1α induces migration of a cell population in peripheral blood that is enriched for cells with the capacity to differentiate into mast cells. These findings thus suggest a mechanism by which human mast cell progenitors may be recruited from circulation into the tissue.

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TGF-β1 enhances SDF-1α-induced chemotaxis and homing of naive T cells by up-regulating CXCR4 expression and downstream cytoskeletal effector molecules

et al. 2002

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The migration of immunocytes within the extracellular matrix (ECM) is influenced by the activation state of the incoming cell and its responses to the presence of chemokines and cytokines. We studied the regulatory role of TGF‐β1 on T cell homing to secondary lymphatic organs, such as the spleen, and chemotaxis within an ECM‐like environment in using an ECM‐like 3‐dimensional gel system designed to follow the migration of individual leukocytes along chemokine gradients in real time. The numbers of migrating naive, but not memory T cells toward SDF‐1α markedly increased after pre‐incubating the cells with TGF‐β1 (0.25 ng / ml) for 24 h. The mechanisms underlying TGFβ1‐modulated migration involve the up‐regulation of the expression of the SDF‐1α receptor CXCR4, the enhancement of the SDF‐1α‐induced actin polymerization, and increased phosphorylation of Pyk2, a focal adhesion kinase involved in integrin‐mediated lymphocyte migration, adhesion and interactions with ECM. Interestingly, priming of naive human T cells with TGF‐β1 increased homing of these cells to the spleen of NOD / SCID mice in a CXCR4‐dependent manner. We propose that theeffect of TGF‐β1 on the chemotaxis of naive T cells may be important in the locomotion of naive T cells toward SDF‐1α‐rich niches.

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Human mast cell migration in response to members of the transforming growth factor-β family

Cited by 113 publications

References 35 publications

Correlation between transforming growth factor-beta expression and mast cell count in different grades of oral submucous fibrosis

Correlation between transforming growth factor-beta expression and mast cell count in different grades of oral submucous fibrosis

The chemokine receptor CXCR4 is expressed within the mast cell lineage and its ligand stromal cell-derived factor-1α acts as a mast cell chemotaxin

TGF-β1 enhances SDF-1α-induced chemotaxis and homing of naive T cells by up-regulating CXCR4 expression and downstream cytoskeletal effector molecules

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