Human mastadenovirus in water, sediment, sea surface microlayer, and bivalve mollusk from southern Brazilian beaches

Gularte, Juliana Schons; Girardi, Viviane; Demoliner, Meriane; Souza, Fernanda Gil de; Filippi, Micheli; Eisen, Ana Karolina Antunes; Mena, Kristina D.; Quevedo, Daniela Müller de; Rigotto, Caroline; Barros, Marcelo Pereira de; Spilki, Fernando Rosado

doi:10.1016/j.marpolbul.2018.12.046

Cited by 21 publications

(18 citation statements)

References 54 publications

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“…Quantitative microbial risk assessment (QMRA) is a useful tool that has been used for almost 40 years to estimate human health risks associated with exposures to pathogens in different environmental matrices ( Haas et al, 2014 ). QMRA has been applied to assess health risks associated with bioaerosols ( Carducci et al, 2018 ), drinking water ( Petterson and Ashbolt, 2016 ), reclaimed water ( Zaneti et al, 2012a , Zaneti et al, 2012b , Zaneti et al, 2013 ), recreational waters ( Girardi et al, 2019 ; Gularte et al, 2019 ), irrigation water ( Ezzat, 2020 ), and sewage ( Kozak et al, 2020 ). Recent publications have encouraged the application of QMRA based on previous studies of relevant respiratory viruses, such as SARS-CoV and MERS-CoV, to assess the likelihood of adverse effects of SARS-CoV-2 associated with sewage exposure ( Haas, 2020 ; Kitajima et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants

Zaneti¹,

Girardi

Spilki

et al. 2021

Science of The Total Environment

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117

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Faecal-oral transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is yet to be validated, but it is a critical issue and additional research is needed to elucidate the risks of the novel coronavirus in sanitation systems. This is the first study that investigates the potential health risks of SARS-CoV-2 in sewage to wastewater treatment plant (WWTP) workers. A quantitative microbial risk assessment (QMRA) is applied for three COVID-19 scenarios (moderate, aggressive and extreme) to study the effects of different stages of the pandemic in terms of percentage of infected population on the probability of infection to WWTP workers. A dose-response model for SARS-CoV-1 (as a surrogate pathogen) is assumed in the QMRA for SARS-CoV-2 using an exponential model with k = 4.1 × 10 2 . Literature data are incorporated to inform assumptions for calculating the viral load, develop the model, and derive a tolerable infection risk. Results reveal that estimates of viral RNA in sewage at the entrance of WWTPs ranged from 4.14 × 10 1 to 5.23 × 10 3 GC·mL −1 (viable virus concentration from 0.04 to 5.23 PFU·mL −1 , respectively). In addition, estimated risks for the aggressive and extreme scenarios (2.6 × 10 −3 and 1.3 × 10 −2 , respectively) were likely to be above the derived tolerable infection risk for SARS-CoV-2 of 5.5 × 10 −4 pppy, thus reinforcing the concern of sewage systems as a possible transmission pathway of SARS-CoV-2. These findings are helpful as an early health warning tool and in prioritizing upcoming risk management strategies, such as Emergency Response Plans (ERPs) for water and sanitation operators during the COVID-19 and future pandemics.

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Section: Introductionmentioning

confidence: 99%

Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants

Zaneti¹,

Girardi

Spilki

et al. 2021

Science of The Total Environment

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117

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“…A549 cell culture was used to value the presence of HAdV infectious particles in samples following the same protocol described by Gularte et al [15] with some modifications. Briefly, 24-well plates were used to carry out the assays.…”

Section: Viral Isolationmentioning

confidence: 99%

“…The fecal-oral route is the main route of HAdV transmission; some serotypes can be eliminated in feces of the host asymptomatically and excreted for months and even years [8,9,11]. This enteric virus is used as a reliable indicator of fecal contamination in different aquatic environments [12][13][14][15]. Its wide use in fecal contamination monitoring is due to its greater stability under adverse conditions, such as physical and chemical treatments, UV light, and pH and temperature changes [16].…”

Section: Introductionmentioning

confidence: 99%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.

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“…The qPCR reactions were performed using the VTB1 and VTB2 oligonucleotides pairs (Wolf, Hewitt, & Greening, 2010), and following the volumes and reaction steps previously established (Gularte et al, 2019). For detecting the presence of the AdV genome of different hosts, it was used the region that codifies the DNA-polymerase using pan-adenovirus oligonucleotide external pair (Pol-F and Pol-R) and oligonucleotide internal pair (Pol-nF and Pol-nR) (Li, He, & Jiang, 2010), through of a nested-PCR (Gularte et al, 2019). For the phylogenetic analysis, the DNA sequencing of amplicons obtained from nested-PCR was performed by the Sanger method.…”

Section: Editor's Choice-short Communicationmentioning

confidence: 99%

Occurrence of human adenoviruses in a beach area of Guarujá, São Paulo, Brazil

Roveri

Guimarães

Correia

et al. 2020

Water Environment Research

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Along the coastal zone of the State of São Paulo, Brazil, diffuse discharges that flow directly to tourist beaches are responsible for introducing various pathogens into recreational waters. The objective of this study was to analyze, for the first time, the presence of protozoa (Cryptosporidium ssp and Giardia ssp), as well as human mastadenoviruses (HAdV–species C and F) and other species of adenoviruses (AdV) in beach drainage channels of Enseada and Perequê, municipality of Guarujá, São Paulo, Brazil. Protozoa were not detected in any sample over the course of the 11‐month study. In relation to HAdVs, 100% (n = 22) of the water samples presented contamination by at least one type of virus (C, D, or F species), suggesting potential risks to the public health following recreational exposure of beach users to these waters. Practitioner points First report on the presence of human adenoviruses in urban drainage channels in the coast of São Paulo State, Brazil. Urban surface water runoff is responsible for introducing human adenoviruses linked to disease outbreaks in areas of intense recreation. Cryptosporidium oocysts and Giardia cysts were below the limit of detection for all analyzed samples. However, all sites were positive for at least one of the viral species (HAdV–C, HAdV–D, or HAdV–F). Viral loads found in the water were similar to those commonly found in the wastewater.

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Human mastadenovirus in water, sediment, sea surface microlayer, and bivalve mollusk from southern Brazilian beaches

Cited by 21 publications

References 54 publications

Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants

Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Occurrence of human adenoviruses in a beach area of Guarujá, São Paulo, Brazil

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