Human Mesenchymal Stem Cells Expansion on Three-Dimensional (3D) Printed Poly-Styrene (PS) Scaffolds in a Perfusion Bioreactor

Kumar, Arun; Lau, Wing Hung; Starly, Binil

doi:10.1016/j.procir.2017.04.012

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…O 2 ‐treated scaffolds demonstrate a method to transform PS to a 3D culture surface which hMSCs attach and grow with future work seeking to investigate this further. Extrusion 3D‐printed PS scaffolds have previously isolated and expanded lymphoma cancer cells, with the scaffold isolating the cells of interest and PS punched scaffold have expanded hMSCs in a perfusion bioreactor . Continued customization of PS scaffolds would define surface chemistry, geometry, and flow properties for individual cell populations.…”

Section: Discussionmentioning

confidence: 99%

“…The widespread use of PS as a 2D culture substrate, and demonstrated improvements 3D culture provides, suggests transitioning PS to a 3D culture platform. However, the applications of 3D‐printed PS as a cell‐contacting growth surface have been limited, likely due to the difficulty in liquefying PS without thermally degrading the polymer . Previous approaches tend to rely on fabricating large objects by sintering smaller objects (e.g., a packed‐bead bioreactor) or microfluidic approaches .…”

Section: Introductionmentioning

confidence: 99%

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Development of surface functionalization strategies for 3D‐printed polystyrene constructs

et al. 2019

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There is a growing interest in 3D printing to fabricate culture substrates; however, the surface properties of the scaffold remain pertinent to elicit targeted and expected cell responses. Traditional 2D polystyrene (PS) culture systems typically require surface functionalization (oxidation) to facilitate and encourage cell adhesion. Determining the surface properties which enhance protein adhesion from media and cellular extracellular matrix (ECM) production remains the first step to translating 2D PS systems to a 3D culture surface. Here we show that the presence of carbonyl groups to PS surfaces correlated well with successful adhesion of ECM proteins and sustaining ECM production of deposited human mesenchymal stem cells, if the surface has a water contact angle between 50° and 55°. Translation of these findings to custom‐fabricated 3D PS scaffolds reveals carbonyl groups continued to enhance spreading and growth in 3D culture. Cumulatively, these data present a method for 3D printing PS and the design considerations required for understanding cell–material interactions. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2566–2578, 2019.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of surface functionalization strategies for 3D‐printed polystyrene constructs

et al. 2019

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“…17 Although a large number of experimental studies have been conducted on bioreactor systems, 21–23 limited information has been reported on computational modeling of fluid environment prior to their application. 13,18,20,24–26 Multiple factors are inserted during modeling of a dynamic systems, including fluid flow rate, size and pattern of scaffold, structure of fluid-containing chamber, entrance and exit zone of medium, flow direction and shear stress. 19,20,27 However, shear stress is affected by all of previous mentioned parameters; hence, it is the most important and the only comparable factor.…”

Section: Introductionmentioning

confidence: 99%

Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering

Nokhbatolfoghahaei

Bohlouli

Adavi

et al. 2020

Proc Inst Mech Eng H

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Bioreactor system has been used in bone tissue engineering in order to simulate dynamic nature of bone tissue environments. Perfusion bioreactors have been reported as the most efficient types of shear-loading bioreactor. Also, combination of forces, such as rotation plus perfusion, has been reported to enhance cell growth and osteogenic differentiation. Mathematical modeling using sophisticated infrastructure processes could be helpful and streamline the development of functional grafts by estimating and defining an effective range of bioreactor settings for better augmentation of tissue engineering. This study is aimed to conduct computational modeling for newly designed bioreactors in order to alleviate the time and material consuming for evaluating bioreactor parameters and effect of fluid flow hydrodynamics (various amounts of shear stress) on osteogenesis. Also, biological assessments were performed in order to validate similar parameters under implementing the perfusion or rotating and perfusion fluid motions in bioreactors’ prototype. Finite element method was used to investigate the effect of hydrodynamic of fluid flow inside the bioreactors. The equations used in the simulation to calculate the velocity values and consequently the shear stress values include Navier–Stokes and Brinkman equations. It has been shown that rotational fluid motion in rotating and perfusion bioreactor produces more velocity and shear stress compared with perfusion bioreactor. Moreover, implementing the perfusion together with rotational force in rotating and perfusion bioreactors has been shown to have more cell proliferation and higher activity of alkaline phosphatase enzyme as well as formation of extra cellular matrix sheet, as an indicator of bone-like tissue formation.

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Chitinous Scaffolds from Marine Sponges for Tissue Engineering

Mutsenko

Gryshkov

Rogulska

et al. 2019

Springer Series in Biomaterials Science and Engineering

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Human Mesenchymal Stem Cells Expansion on Three-Dimensional (3D) Printed Poly-Styrene (PS) Scaffolds in a Perfusion Bioreactor

Cited by 6 publications

References 8 publications

Development of surface functionalization strategies for 3D‐printed polystyrene constructs

Development of surface functionalization strategies for 3D‐printed polystyrene constructs

Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering

Chitinous Scaffolds from Marine Sponges for Tissue Engineering

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