Human Metaplastic Breast Carcinoma and Decorin

Boström, Pia; Sainio, Annele; Eigėlienė, Natalja; Jokilammi, Anne; Elenius, Klaus; Koskivuo, Ilkka; Järveläinen, Hannu

doi:10.1007/s12307-017-0195-8

Cited by 10 publications

(12 citation statements)

References 83 publications

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“…Caspase‐8 is able to both induce cell death directly by activating effector caspases and by initiating other apoptotic cascades. In our own studies, we have achieved oncosuppression in different carcinoma cells using recombinant human decorin cDNA‐based adenoviral vector (Boström et al, ; Sainio et al, ; Nyman et al, ; Boström et al, ). Although the precise mechanisms behind the observed oncosuppression are not yet known, our preliminary results indicate among other things induction and involvement of anti‐tumorigenic microRNAs.…”

Section: Decorin – Structure Interactions and Functionsmentioning

confidence: 99%

Decorin‐mediated oncosuppression – a potential future adjuvant therapy for human epithelial cancers

Sainio

Järveläinen

2018

British J Pharmacology

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Currently, the multifaceted role of the extracellular matrix (ECM) in tumourigenesis has been realized. One ECM macromolecule exhibiting potent oncosuppressive actions in tumourigenesis is decorin, the prototype of the small leucine‐rich proteoglycan gene family. The actions of decorin include its ability to function as an endogenous pan‐receptor tyrosine kinase inhibitor, a regulator of both autophagy and mitophagy, as well as a modulator of the immune system. In this review, we will discuss these topics in more detail. We also provide a summary of preclinical studies exploring the value of decorin‐mediated oncosuppression, as a potential future adjuvant therapy for epithelial cancers.Linked ArticlesThis article is part of a themed section on Translating the Matrix. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v176.1/issuetoc

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Section: Decorin – Structure Interactions and Functionsmentioning

confidence: 99%

Decorin‐mediated oncosuppression – a potential future adjuvant therapy for human epithelial cancers

Sainio

Järveläinen

2018

British J Pharmacology

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“…The results of this study are consistent with our previous studies demonstrating that malignant cells of various other human epithelial cancers, namely human breast, bladder, and colon carcinomas lack decorin expression. [17][18][19][20] The expression of decorin was further studied in cultured human primary and commercial vulva carcinoma cell lines. In agreement with the above tissue sample data, no decorin expression could be detected in these cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…15 , 16 We have previously shown that human breast, bladder, and colon cancer cells do not express decorin. [17][18][19][20] We have also shown that adenovirus-mediated decorin cDNA (Ad-DCN) transduction of cells representing the above cancers markedly decreases their malignant behavior. [17][18][19][20] Furthermore, in our recent study, we have even been able to demonstrate that Ad-DCN transduction alters the cytological features of human metaplastic breast carcinoma tissue toward a less malignant phenotype.…”

Section: Introductionmentioning

confidence: 99%

“…[17][18][19][20] We have also shown that adenovirus-mediated decorin cDNA (Ad-DCN) transduction of cells representing the above cancers markedly decreases their malignant behavior. [17][18][19][20] Furthermore, in our recent study, we have even been able to demonstrate that Ad-DCN transduction alters the cytological features of human metaplastic breast carcinoma tissue toward a less malignant phenotype. 20 Vulva carcinoma, frequently associated with human papilloma virus (HPV), is the fourth most common gynecological cancer, and its incidence in women under 60 years is increasing worldwide.…”

Section: Introductionmentioning

confidence: 99%

“…[17][18][19][20] Furthermore, in our recent study, we have even been able to demonstrate that Ad-DCN transduction alters the cytological features of human metaplastic breast carcinoma tissue toward a less malignant phenotype. 20 Vulva carcinoma, frequently associated with human papilloma virus (HPV), is the fourth most common gynecological cancer, and its incidence in women under 60 years is increasing worldwide. 21 Although the initial prognosis of early stages of vulva carcinoma is good, improvements in the treatment of this disease in advanced stages and in its recurrence are needed.…”

Section: Introductionmentioning

confidence: 99%

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Decorin Expression in Human Vulva Carcinoma: Oncosuppressive Effect of Decorin cDNA Transduction on Carcinoma Cells

Nyman

Jokilammi

Boström

et al. 2019

J Histochem Cytochem.

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The extracellular matrix proteoglycan decorin is well-known for its oncosuppressive activity. Here, decorin expression was examined in human vulva carcinoma tissue samples and in primary and commercial cell lines representing this malignant disease. Furthermore, the effect of adenovirus-mediated decorin cDNA (Ad-DCN) transduction on the viability, proliferation, and the expression and activity of the epidermal growth factor receptor (ErbB/HER) family members of the cell lines were investigated. Using in situ hybridization and immunohistochemistry for decorin, it was demonstrated that malignant cells in human vulva carcinoma tissues lack decorin expression. This result was true independently on tumor stage, grade or human papillomavirus status. RT-qPCR analyses showed that the human vulva carcinoma cell lines used in this study were also negative for decorin expression. Transduction of the cell lines with Ad-DCN caused a marked reduction in cell viability, while the proliferation of the cells was not affected. Experiments examining potential mechanisms behind the oncosuppressive effect of Ad-DCN transduction revealed that ErbB2/HER2 expression and activity in carcinoma cells were markedly downregulated. In conclusion, the results of this study showed that human vulva carcinoma cells lack decorin expression, and that Ad-DCN transduction of these cells induces oncosuppressive activity in part via downregulation of ErbB2/HER2.

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A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate

Jyothsna

Sarkar

Jha

et al. 2021

J Biomedical Materials Res

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Collagen I, the most abundant extracellular matrix (ECM) protein in vertebrate tissues provides mechanical durability to tissue microenvironments and regulates cell function. Its fibrillogenesis in biological milieu is predominantly regulated by dermatan sulfate proteoglycans, proteins conjugated with iduronic acid-containing dermatan sulfate (DS) glycosaminoglycans (GAG). Although DS is known to regulate tissue function through its modulation of Coll I architecture, a precise understanding of the latter remains elusive. We investigated this problem by visualizing the fibrillar pattern of fixed Coll I gels polymerized in the presence of varying concentrations of DS using second harmonic generation microscopy. Measuring mean second harmonic generation signal (which estimates the ordering of the fibrils), and surface occupancy (which estimates the space occupied by fibrils) supported by confocal reflectance microscopy, our observations indicated that the effect on fibril pattern of DS is contextual upon the latter's concentrations: Lower levels of DS resulted in sparse disorganized fibrils; higher levels restore organization, with fibrils occupying greater space.An appropriate change in elasticity as a result of DS levels was also observed through atomic force microscopy. Examination of dye-based GAG staining and scanning electron microscopy suggested distinct constitutions of Coll I gels when polymerized with higher and lower levels of DS. We observed that adhesion of the invasive ovarian cancer cells SKOV3 decreased for lower DS levels but was partially restored at higher DS levels. Our study shows how the Coll I gel pattern-tuning of DS is of relevance for understanding its biomaterial applications and possibly, pathophysiological functions.

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Human Metaplastic Breast Carcinoma and Decorin

Cited by 10 publications

References 83 publications

Decorin‐mediated oncosuppression – a potential future adjuvant therapy for human epithelial cancers

Decorin‐mediated oncosuppression – a potential future adjuvant therapy for human epithelial cancers

Decorin Expression in Human Vulva Carcinoma: Oncosuppressive Effect of Decorin cDNA Transduction on Carcinoma Cells

A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate

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