Human Metapneumovirus Infection among Children, Bangladesh

Brooks, W. Abdullah; Erdman, Dean D.; Terebuh, Pauline; Klimov, Alexander; Goswami, Doli; Sharmeen, Amina Tahia; Azim, Tasnim; Luby, Stephen P.; Bridges, Carolyn B.; Breiman, Robert F.

doi:10.3201/eid1310.070337

Cited by 24 publications

(24 citation statements)

References 12 publications

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“…Several studies have demonstrated that human metapneumovirus is more likely to be associated with bronchiolitis in early childhood [Xepapadaki et al, 2004;Garcia-Garcia et al, 2007;do Carmo Debur et al, 2007]. However, other investigations including the present study found that human metapneumovirus was associated more frequently with bronchopneumonia compared to human metapneumovirus-bronchiolitis cases [Choi et al, 2006;Brooks et al, 2007]. These variable results may reflect that human metapneumovirus is not exclusively a bronchiolitis pathogen and can, also cause bronchopneumonia among infants.…”

Section: Discussioncontrasting

confidence: 55%

“…Human metapneumovirus was discovered recently as a respiratory virus implicated in both upper and lower respiratory tract infection ranging from mild to severe disease in all age groups [van den Hoogen et al, 2001]. Since then, it has been reported in Europe, Asia, Australia, South Africa and America [Peret et al, 2002;Bastien et al, 2003;Jpma et al, 2004;Rao et al, 2004;Sloots et al, 2006;Brooks et al, 2007], suggesting worldwide distribution. Seroprevalence studies have shown that this virus has been present among humans for over five decades [van den Hoogen et al, 2001;Hamelin et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of human metapneumovirus among hospitalized children younger than 1 year in Catalonia, Spain

Camps

Ricart

Dimova

et al. 2008

Journal of Medical Virology

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Human metapneumovirus was discovered recently respiratory virus implicated in both upper and lower respiratory tract infection. In children, the clinical symptoms of human metapneumovirus are similar to those produced by respiratory syncytial virus, ranging from mild to severe diseases such as bronchiolitis and pneumonia. The aim of the present study was to describe the prevalence of human metapneumovirus and other common respiratory viruses among admitted to hospital infants. From January 2006 to June 2006, 99 nasopharyngeal aspirates were collected from hospitalized children younger than 12 months in order to study respiratory viruses. Human metapneumovirus detection was performed by cell culture and two RT-PCR targeting on polymerase and fusion genes. The latter gene was used for phylogenetic analysis. In 67/99 children (67%) at least one viral pathogen was identified, the viruses detected most frequently were respiratory syncytial virus (35%), human metapneumovirus (25%) and rhinovirus (19%). The results obtained in this study, show that: (1) human metapneumovirus is one of the most important viruses among children less than 12 months; (2) children infected with human metapneumovirus were significantly older than those infected by respiratory syncytial virus; (3) human metapneumovirus was associated more frequently with pneumonia whereas respiratory syncytial virus was only detected in patients with bronchiolitis; (4) there was a clear epidemiological succession pattern with only a small overlap among the viruses detected most frequently; (5) all human metapneumovirus samples were clustered within sublineage A2.

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Prevalence of human metapneumovirus among hospitalized children younger than 1 year in Catalonia, Spain

Camps

Ricart

Dimova

et al. 2008

Journal of Medical Virology

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“…To test retrospectively for viral infection, serological assays can be performed using acute-and convalescentstage patient serum samples. For hMPV, tests have included immunofluorescence assays using hMPVinfected cells [11,15] or recombinant baculovirus-infected cells expressing the fusion (F) protein [16] as well as ELISAs using hMPV-infected cell lysates [17,18], lysates from recombinant baculovirus-infected cells expressing nucleoprotein [19], purified nucleoprotein from a prokaryotic expression system [20], and a recombinant vesicular stomatitis virus expressing hMPV F protein [6]. However, to our knowledge, no serological studies have been conducted using purified hMPV glycoproteins from a eukaryotic or human cell line system.…”

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confidence: 99%

Human Metapneumovirus Reinfection among Children in Thailand Determined by ELISA Using Purified Soluble Fusion Protein

Pavlin

Hickey

Ulbrandt³

et al. 2008

J INFECT DIS

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Background Human metapneumovirus (hMPV) is a newly discovered paramyxovirus that causes acute respiratory illness. Despite apparent near-universal exposure during early childhood, immunity is transient. Methods An indirect screening ELISA using a recombinant, soluble, fusion (F) glycoprotein derived from hMPV was used to test for anti-F IgG in 1,380 acute and convalescent sera collected from children in Kamphaeng Phet, Thailand Results 1,376 (99.7%) tested sera showed evidence of prior infection with hMPV. 67 children demonstrated a four-fold or greater rise in titer for an overall re-infection rate of 4.9%. Two children demonstrated evidence of an initial infection. 49 of the 69 new or re-infections occurred in 2000, accounting for 13.2% of all non-flaviviral febrile illnesses in the study population in that year. Of 69 positive cases, 89.9% reported a respiratory symptom compared to 69.2% of tested negative cases (p<.001). All positive specimens were also tested for an increase in titer to RSV F and 27% exhibited a four-fold or greater rise in titer. Conclusion These results demonstrate hMPV reinfection causing illness at rates equal to that seen for initial infections. hMPV may represent a more significant impact in older children than previously realized and may be the cause of significant outbreaks.

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“…Most hMPV infections occur within the first few years of life, usually later than those for hRSV, and cause 5 to 10% of respiratory infections in children requiring hospitalization, second only to hRSV (7). Both viruses are ubiquitous globally, and recent studies have documented a high prevalence of infection among children in less developed countries (8,9). Clinical outcomes similar to those for hRSV have been described for hMPV infections in the frail elderly (10).…”

mentioning

confidence: 74%

“…As part of a population-based respiratory disease surveillance study in Dhaka, Bangladesh, conducted by the International Center for Diarrheal Disease Research, Bangladesh (ICDDR, B), paired acute-and convalescent-phase serum specimens were collected from children Ͻ5 years of age presenting with acute respiratory tract illness and/or fever from April 2004 to Feb 2006. Serum pairs obtained from 852 children were sent to the Centers for Disease Control and Prevention (CDC; Atlanta, Georgia, USA) for serologic testing for respiratory pathogens (8). This study was reviewed and approved by the Research Review and Ethical Review Committees of ICDDR, B and the CDC Institutional Review Board.…”

Section: Methodsmentioning

confidence: 99%

Serologic Cross-Reactions between Nucleocapsid Proteins of Human Respiratory Syncytial Virus and Human Metapneumovirus

et al. 2015

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Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) share virologic and epidemiologic features and cause clinically similar respiratory illness predominantly in young children. In a previous study of acute febrile respiratory illness in Bangladesh, we tested paired serum specimens from 852 children presenting fever and cough for diagnostic increases in titers of antibody to hRSV and hMPV by enzyme immunoassay (EIA). Unexpectedly, of 93 serum pairs that showed a >4-fold increase in titers of antibody to hRSV, 24 (25.8%) showed a concurrent increase in titers of antibody to hMPV; of 91 pairs showing an increase to hMPV, 13 (14.3%) showed a concurrent increase to hRSV. We speculated that common antigens shared by these viruses explain this finding. Since the nucleocapsid (N) proteins of these viruses show the greatest sequence homology, we tested hyperimmune antisera prepared for each virus against baculovirus-expressed recombinant N (recN) proteins for potential cross-reactivity. The antisera were reciprocally reactive with both proteins. To localize common antigenic regions, we first expressed the carboxy domain of the hMPV N protein that was the most highly conserved region within the hRSV N protein. Although reciprocally reactive with antisera by Western blotting, this truncated protein did not react with hMPV IgG-positive human sera by EIA. Using 5 synthetic peptides that spanned the amino-terminal portion of the hMPV N protein, we identified a single peptide that was cross-reactive with human sera positive for either virus. Antiserum prepared for this peptide was reactive with recN proteins of both viruses, indicating that a common immunoreactive site exists in this region.H uman respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) are negative single-stranded, enveloped RNA viruses that are coclassified within the Pneumovirinae subfamily of the Paramyxoviridae. hRSV is the leading cause of severe lower respiratory tract infections in infants and young children and has been associated with community-acquired pneumonia in adults, with the highest mortality rates found among the hospitalized elderly and immunocompromised (1, 2, 3). hMPV was identified first in 2001 by van den Hoogen et al. (4) and subsequently has been shown to have clinical and epidemiological features similar to but distinct from those of hRSV (5, 6). Most hMPV infections occur within the first few years of life, usually later than those for hRSV, and cause 5 to 10% of respiratory infections in children requiring hospitalization, second only to hRSV (7). Both viruses are ubiquitous globally, and recent studies have documented a high prevalence of infection among children in less developed countries (8, 9). Clinical outcomes similar to those for hRSV have been described for hMPV infections in the frail elderly (10).The RNA genomes of hRSV and hMPV are complexed with nucleocapsid (N) protein that together serve as the templates for genome replication and transcription (11). The N protein is the most abundant...

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Human Metapneumovirus Infection among Children, Bangladesh

Cited by 24 publications

References 12 publications

Prevalence of human metapneumovirus among hospitalized children younger than 1 year in Catalonia, Spain

Prevalence of human metapneumovirus among hospitalized children younger than 1 year in Catalonia, Spain

Human Metapneumovirus Reinfection among Children in Thailand Determined by ELISA Using Purified Soluble Fusion Protein

Serologic Cross-Reactions between Nucleocapsid Proteins of Human Respiratory Syncytial Virus and Human Metapneumovirus

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