Human Milk Glycoproteins Inhibit the Adherence of <i>Salmonella typhimurium</i> to HeLa Cells

Bessler, H. C.; Oliveira, Inaiara Rosa de; Giugliano, Loreny Gimenes

doi:10.1111/j.1348-0421.2006.tb03863.x

Cited by 39 publications

(21 citation statements)

References 27 publications

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“…We found a significant difference in mortality between the LF and control groups supporting the protective effect of LF in this bacterial infection. The second significant result was the change in weight and clinical signs of acute infection (piloerection, hunched position and reduced movement) due to enterocolitis in the LF group in concordance with previous reports (Bessler et al 2006;Ochoa and Cleary, 2009). The third significant result found was the difference in the histopathology of the four organs studied between LF and control group.…”

Section: Discussionsupporting

confidence: 86%

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Effect of bovine lactoferrin in Salmonella ser. Typhimurium infection in mice

et al. 2010

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Lactoferrin (LF) has in vitro antimicrobial activity against Gram-negative bacteria. Salmonella enterica subsp. enterica serovar Typhimurium causes systemic infection and acute diarrhea in humans, mainly in children younger than 2 years of age. The aim of the study was to determine the in vivo effect of bovine LF in Salmonella ser. Typhimurium infection in mice. 58 BALB/c mice were employed. Two hours before the infection with 300 microl of 10(7) CFU of Salmonella ser. Typhimurium, 29 mice received LF (2 mg) and 29 placebo (buffer). After the infection, the mice received LF (10 mg/ml) ad libitum or buffer, respectively, for 7 days. Mortality, weight and clinical signs (piloerection, hunched position and reduced movement) were monitored daily. The degree of inflammation and necrosis in the intestine, liver, spleen and brain were studied with a blinded observer. The mortality in the control group (8/29) was higher than in the LF group (1/29) (Kapplan Meier P < 0.05). From the third day post-infection the control group were significantly more symptomatic (P < 0.05). The blood culture for Salmonella spp. was positive for all mice studied in the control group (17/17), but positive in the LF group in only 6/17 animals (P < 0.05). In the LF group, the pathologic studies show less inflammation and focal necrosis in the four organs studied, with the greatest difference found in the intestine. Bovine LF protects against Salmonella ser. Typhimurium infection in mice, reducing the severity, mortality and the degree of inflammation of this infection.

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Section: Discussionsupporting

confidence: 86%

“…enterica serovar Typhimurium and reduces their ability to adhere to host cells in an in vitro model (Bessler et al 2006). In vivo, the LF effect in a Salmonella ser.…”

Section: Introductionmentioning

confidence: 99%

Effect of bovine lactoferrin in Salmonella ser. Typhimurium infection in mice

et al. 2010

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“…Thus, the effect of dimeric IgA antibodies produced locally would promote the inhibition of excessive colonization of microorganisms from the mucous membrane, as well as the penetration of soluble antigens. Free secretory component Free secretory component (SC), which is particularly abundant in breast milk, can block epithelial adhesion and therefore limit infection by enterotoxigenic Escherichia coli, 16 Salmonella typhimurium, 17 and pneumococcus, 18 as well as inhibit the effect of bacterial toxins such as Clostridium difficile toxin A. 19 Lysozyme and lactoferrin Major enzymes present in colostrum include lysozyme and lactoferrin.…”

Section: B Cellsmentioning

confidence: 99%

Immunology of breast milk

Palmeira

Carneiro‐Sampaio

2016

Rev. Assoc. Med. Bras.

212

175

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In the critical phase of immunological immaturity of the newborn, particularly for the immune system of mucous membranes, infants receive large amounts of bioactive components through colostrum and breast milk. Colostrum is the most potent natural immune booster known to science. Breastfeeding protects infants against infections mainly via secretory IgA (SIgA) antibodies, but also via other various bioactive factors. It is striking that the defense factors of human milk function without causing inflammation; some components are even anti-inflammatory. Protection against infections has been well evidenced during lactation against, e.g., acute and prolonged diarrhea, respiratory tract infections, including otitis media, urinary tract infection, neonatal septicemia, and necrotizing enterocolitis. The milk's immunity content changes over time.In the early stages of lactation, IgA, anti-inflammatory factors and, more likely, immunologically active cells provide additional support for the immature immune system of the neonate. After this period, breast milk continues to adapt extraordinarily to the infant's ontogeny and needs regarding immune protection and nutrition. The need to encourage breastfeeding is therefore justifiable, at least during the first 6 months of life, when the infant's secretory IgA production is insignificant.

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“…Bovine lactoferrin (10e20% iron saturated) has an iron-dependent bacteriostatic effect on Salmonella typhimurium [33]. Bovine and human lactoferrin inhibit adherence and invasion of Salmonella to tissue culture cells [33,34].…”

Section: Salmonellamentioning

confidence: 99%

Effect of lactoferrin on enteric pathogens

Ochoa

Cleary²

2009

Biochimie

120

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Much has been learned in recent years about the mechanisms by which breastfeeding improves child health and survival. However, there has been little progress in using these insights to improve pediatric care. Factors that are important for protecting the breast fed infant might be expected to decrease the adverse effects of weaning on diarrhea, growth, and development. Lactoferrin, an ironbinding protein with multiple physiological functions (anti-microbial, anti-inflammatory, and immunomodulatory), is one of the most important proteins present in mammalian milk. Protection against gastroenteritis is the most likely biologically relevant activity of lactoferrin. Multiple in vitro and animal studies have shown a protective effect of lactoferrin on infections with enteric microorganisms, including rotavirus, Giardia, Shigella, Salmonella and the diarrheagenic Escherichia coli. Lactoferrin has two major effects on enteric pathogens: it inhibits growth and it impairs function of surface expressed virulence factors thereby decreasing their ability to adhere or to invade mammalian cells. Thus, lactoferrin may protect infants from gastrointestinal infection by preventing the attachment by enteropathogens in the gut. Recently several clinical trials in children have started to address this issue. Whether lactoferrin can prevent a significant portion of diarrheal disease remains to be determined.

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Human Milk Glycoproteins Inhibit the Adherence of Salmonella typhimurium to HeLa Cells

Cited by 39 publications

References 27 publications

Effect of bovine lactoferrin in Salmonella ser. Typhimurium infection in mice

Effect of bovine lactoferrin in Salmonella ser. Typhimurium infection in mice

Immunology of breast milk

Effect of lactoferrin on enteric pathogens

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