Human Milk Oligosaccharides Protect Bladder Epithelial Cells Against Uropathogenic Escherichia coli Invasion and Cytotoxicity

Lin, Ann E.; Autran, Chloe; Espanola, Sophia D.; Bode, Lars; Nizet, Victor

doi:10.1093/infdis/jit464

Cited by 76 publications

(61 citation statements)

References 54 publications

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“…As a result epithelial cells cannot respond to E.coli bacteria. These effects were only observed with the HMO 3 0 -sialyllactose (3 0 SL) (Lin et al 2014). 2 0 FL HMOs can directly influence inflammatory reactions as well (He et al 2014).…”

Section: Effects Of Hmos On Prevention Of Infection and Support Of Immentioning

confidence: 98%

“…Presence of HMOs in urine of infants indicates that HMOs are absorbed into the bloodstream (Rudloff et al 2012). Blood absorbed HMOs have been shown to reduce invasion of uropathogenic Escherichia coli (E. coli) by interacting with ureter epithelial cells, making them more resistant against E. coli invasion by a suppression of intracellular signaling of apoptotic pathways (Lin et al 2014). As a result epithelial cells cannot respond to E.coli bacteria.…”

Section: Effects Of Hmos On Prevention Of Infection and Support Of Immentioning

confidence: 99%

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Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Akkerman

Faas²,

Vos

2018

Critical Reviews in Food Science and Nutrition

139

140

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Human milk (HM) is the golden standard for nutrition of newborn infants. Human milk oligosaccharides (HMOs) are abundantly present in HM and exert multiple beneficial functions, such as support of colonization of the gut microbiota, reduction of pathogenic infections and support of immune development. HMO-composition is during lactation continuously adapted by the mother to accommodate the needs of the neonate. Unfortunately, for many valid reasons not all neonates can be fed with HM and are either totally or partly fed with cow-milk derived infant formulas, which do not contain HMOs. These cow-milk formulas are supplemented with non-digestible carbohydrates (NDCs) that have functional effects similar to that of some HMOs, since production of synthetic HMOs is challenging and still very expensive. However, NDCs cannot substitute all HMO functions. More efficacious NDCs may be developed and customized for specific groups of neonates such as pre-matures and allergy prone infants. Here current knowledge of HMO functions in the neonate in view of possible replacement of HMOs by NDCs in infant formulas is reviewed. Furthermore, methods to expedite identification of suitable NDCs and structure/function relationships are reviewed as in vivo studies in babies are impossible.

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Section: Effects Of Hmos On Prevention Of Infection and Support Of Immentioning

confidence: 98%

Section: Effects Of Hmos On Prevention Of Infection and Support Of Immentioning

confidence: 99%

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Akkerman

Faas²,

Vos

2018

Critical Reviews in Food Science and Nutrition

139

140

View full text Add to dashboard Cite

show abstract

“…For example, Lin et al (2014) showed that pooled HMOs protect bladder epithelial cells against uropathogenic Escherichia coli (UPEC) invasion and cytotoxicity. Although the presence of HMOs did not significantly alter the adhesion to the host cells, a significant reduction was observed in bacterial internalization.…”

Section: Structure-function Associations Of Milk Glycansmentioning

confidence: 99%

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Kirmiz

Robinson

Shah

et al. 2018

Annu. Rev. Food Sci. Technol.

106

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Human milk is a unique and complex fluid that provides infant nutrition and delivers an array of bioactive molecules that serve various functions. Glycans, abundant in milk, can be found as free oligosaccharides or as glycoconjugates. Milk glycans are increasingly linked to beneficial outcomes in neonates through protection from pathogens and modulation of the immune system. Indeed, these glycans influence the development of the infant and the infant-gut microbiota. Bifidobacterium species commonly are enriched in breastfed infants and are among a limited group of bacteria that readily consume human milk oligosaccharides (HMOs) and milk glycoconjugates. Given the importance of bifidobacteria in infant health, numerous studies have examined the molecular mechanisms they employ to consume HMOs and milk glycans, thus providing insight into this unique enrichment and shedding light on a range of translational opportunities to benefit at-risk infants.

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“…For example, ␣1-2 fucosylated HMGs, which arise mainly from individuals that are Secretor(ϩ), were observed to prevent the adherence of Campylobacter jejuni to epithelial cells (20) and were associated with protection against diarrhea caused by Campylobacter, caliciviruses, and Escherichia coli toxin in breastfed infants (21)(22)(23). Sialylated HMGs were exclusive receptors for influenza viruses (24 -26) and showed a capacity to inhibit cholera toxin B (27), Vibrio cholera (28), enterotoxigenic E. coli, and uropathogenic E. coli strains (29,30). It was also proposed that HMGs might serve as anti-inflammatory components and thus contribute to the lower incidence of necrotizing enterocolitis in breastfed infants.…”

mentioning

confidence: 99%

Human Milk Contains Novel Glycans That Are Potential Decoy Receptors for Neonatal Rotaviruses

Lasanajak

Song

et al. 2014

Molecular & Cellular Proteomics

117

126

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Human milk contains a rich set of soluble, reducing glycans whose functions and bioactivities are not well understood. Because human milk glycans (HMGs) have been implicated as receptors for various pathogens, we explored the functional glycome of human milk using shotgun glycomics. The free glycans from pooled milk samples of donors with mixed Lewis and Secretor phenotypes were labeled with a fluorescent tag and separated via multidimensional HPLC to generate a tagged glycan library containing 247 HMG targets that were printed to generate the HMG shotgun glycan microarray (SGM). To investigate the potential role of HMGs as decoy receptors for rotavirus (RV), a leading cause of severe gastroenteritis in children, we interrogated the HMG SGM with recombinant forms of VP8* domains of the RV outer capsid spike protein VP4 from human neonatal strains N155 (G10P[11]) and RV3(G3P[6]) and a bovine strain, B223 (G10P[11]). Glycans that were bound by RV attachment proteins were selected for detailed structural analyses using metadataassisted glycan sequencing, which compiles data on each glycan based on its binding by antibodies and lectins before and after exo-and endo-glycosidase digestion of the SGM, coupled with independent MS n analyses. These complementary structural approaches resulted in the identification of 32 glycans based on RV VP8* binding, many of which are novel HMGs, whose detailed structural assignments by MS n are described in a companion report. Although sialic acid has been thought to be important as a surface receptor for RVs, our studies indicated that sialic acid is not required for binding of glycans to individual VP8* domains. Remarkably, each VP8* recognized specific glycan determinants within a unique subset of related glycan structures where specificity differences arise from subtle differences in glycan structures. Molecular & Cellular Proteomics 13:

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Human Milk Oligosaccharides Protect Bladder Epithelial Cells Against Uropathogenic Escherichia coli Invasion and Cytotoxicity

Cited by 76 publications

References 54 publications

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Human Milk Contains Novel Glycans That Are Potential Decoy Receptors for Neonatal Rotaviruses

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