Human monoclonal anti‐D antibodies. I. THEIR PRODUCTION, SEROLOGY, QUANTITATION AND POTENTIAL USE AS BLOOD GROUPING REAGENTS

Kumpel, Belinda M.; Poole, Geoff; Bradley, B. A.

doi:10.1111/j.1365-2141.1989.tb06285.x

Cited by 90 publications

(41 citation statements)

References 13 publications

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“…The production of human monoclonal anti-D antibod ies by long-term culture of Epstein-Barr virus (EBV)-transformed lymphocytes has recently been described [1][2][3][4][5]. In the future, it may be possible to use these antibodies for prophylaxis against haemolytic disease of the newborn.…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

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Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Merry¹,

Brojer²,

Zupanska³

et al. 1989

Vox Sanguinis

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Red cells sensitized with IgGl or IgG3 monoclonal anti-D antibodies were used in rosette assays with human lymphocytes, monocytes and granulocytes. With all three cell types, IgG3 antibodies promoted a greater degree of rosette formation than IgGl antibodies. Monocytes required a minimum of about 0.5 x 10^3 IgG3 molecules per red cell for rosette formation, and granulocytes and lymphocytes required around 1 x 10^4 IgG3 molecules per red cell. Approximately 80% of monocytes and granulocytes and 10% of lymphocytes were capable of rosette formation. These results are consistent with differences in the number and affinity of Fc receptors on different leucocytes. When compared with previous data these results suggest that binding of monocytes to monoclonal anti-D sensitized red cells is very similar to that of red cells sensitized with polyclonal antisera. Lymphocytes and granulocytes, however, appear to bind less well to red cells sensitized with certain monoclonal antibodies than with polyclonal antibodies. These findings may be of relevance to the prophylactic use of monoclonal anti-D antibodies.

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Section: Monoclonal Antibodiesmentioning

confidence: 99%

“…The serological characteristics of some of these antibodies have recently been described [5]. The IgG subclasses were determined by haemagglutination using subclass specific monoclonal antibodies (Unipath).…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Merry¹,

Brojer²,

Zupanska³

et al. 1989

Vox Sanguinis

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“…The MAb-D cell lines were produced by transforming B cells from hyper-immu nised anti-D donors with the Epstein-Barr virus [16,26]. Cells were grown in static culture flasks at 37°C in RPMI 1640 medium contain ing 10% bovine IgG-depleted fetal calf serum (FCS), L-glutamine and antibiotics (Hyclonc Europe Ltd, Cramlington, UK) (RPM1/FCS) for a minimum of 10 days.…”

Section: Antibody Preparationmentioning

confidence: 99%

Quantitation of Rh D Antigen Sites on Weak D and D Variant Red Cells by Flow Cytometry

Jones¹,

Loyd-Evans²,

Kumpel³

1996

Vox Sanguinis

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Information on the number of D sites on weak D (Du) and D variant cells is limited and incomplete. The aim of this study was to use a simple non-isotopic technique utilising a combination of flow cytometry and ELISA to quantitate the number of D sites on an extensive range of these cells. Five human monoclonal IgG anti-D (BRAD-7 (JACK)), BRAD-5, 2B6, BRAD-3, H27) and one affinitypurified polyclonal IgG anti-D were each used at a saturating concentration of 20 pg/ml. In general, BRAD-3, BRAD-5 and 2B6 gave the highest number of D sites per cell (SPC), H27 and the polyclonal anti-D were slightly lower, while BRAD-7 gave the lowest SPC with all D-positive cells tested except DFR. Interestingly, BRAD-7 gave the highest SPC with DFR cells. Rh D antigen density for R(2)R(2) cells was approximately double that seen with either R(2)r or R(0) (presumed R(0)r) cells. R(1)R(1) cells gave only moderately higher SPC than R(1)r cells. Higher SPC were obtained with the R, haplotype if the C^w antigen was present. Weak D, Va and VI cells of the R(1) haplotype had higher SPC than those of the R(0) or R(2) haplotypes. The majority of D variant cells were found to have lower SPC than normal cells, and for polyclonal anti-D, which was the only anti-D to react with all D variants, SPC decreased in the order IIIc>IIIa>HMii>IVa> Va>DFR> DBT>IVb>VII>II>HMi>VI. The number of molecules of IgG anti-D bound to D variant cells varied by up to 10 times with reactive monoclonal antibodies. The highest SPC on weak D and D variant cells were obtained with BRAD-7 (DFR, 9,500), BRAD-3 (Va, 12,500), BRAD-5 (II, 5,500; VII, 5,300; weak D, 1,300), H27 (III, 24,000; VI, 2,900; HMi, 3,000; HMii, 16,800) and polyclonal anti-D (IVa, 9,300; IVb, 4,000; DBT, 4,300).

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“…[9][10][11][12][13] With respect to AITP, however, only one study attempted to treat patients with AITP with MoAnti-D, but platelet counts were not increased. 14 It was suggested that the failure was the result of the monoclonal preparation missing factors present in polyclonal anti-D. 15 Subsequently, few studies attempted to understand why MoAnti-D preparations are not effective in AITP.…”

Section: Introductionmentioning

confidence: 99%

Epitope specificity and isotype of monoclonal anti-D antibodies dictate their ability to inhibit phagocytosis of opsonized platelets

Kjaersgaard

Aslam

Kim³

et al. 2007

Blood

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Rh immune globulin (WinRho SDF; Cangene, Mississauga, ON, Canada) is an effective treatment for autoimmune thrombocytopenic purpura; however, maintaining a sustained supply for its use in autoimmune thrombocytopenic purpura and its primary indication, hemolytic disease of the newborn, makes the development of alternative reagents desirable. We compared Rh immune globulin and 6 human monoclonal anti-D antibodies (MoAnti-D) with differing isotypes and specificities for their ability to opsonize erythrocytes and inhibit platelet phagocytosis in an in vitro assay. Results demonstrated that opsonization of erythrocytes with Rh immune globulin significantly (P < .001) reduced phagocytosis of fluorescently labeled opsonized platelets in an Fcdependent manner. Of the MoAnti-D that shared specificity but differed in isotype, only IgG3 antibodies could significantly (P < .001) inhibit platelet phagocytosis. In contrast, 2 MoAnti-D shared isotypes and differed in specificity; however, only one could significantly (P < .001) inhibit platelet phagocytosis. The results suggest that MoAnti-D epitope specificity and isotypes are critical requirements for optimal inhibition of opsonized platelet phagocytosis.

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Human monoclonal anti‐D antibodies. I. THEIR PRODUCTION, SEROLOGY, QUANTITATION AND POTENTIAL USE AS BLOOD GROUPING REAGENTS

Cited by 90 publications

References 13 publications

Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Quantitation of Rh D Antigen Sites on Weak D and D Variant Red Cells by Flow Cytometry

Epitope specificity and isotype of monoclonal anti-D antibodies dictate their ability to inhibit phagocytosis of opsonized platelets

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