1992
DOI: 10.1073/pnas.89.18.8467
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Human monoclonal islet cell antibodies from a patient with insulin-dependent diabetes mellitus reveal glutamate decarboxylase as the target antigen.

Abstract: The autoimmune phenomena associated with destruction of the (3 cell in pancreatic islets and development of type 1 (insulin-dependent) diabetes mellitus (IDDM) include circulating islet cell antibodies. We have immortalized peripheral blood lymphocytes from prediabetic individuals and patients with newly diagnosed IDDM by Epstein-Barr virus transformation. IgG-positive cells were selected by anti-human IgG-coupled magnetic beads and expanded in cell culture. Supernatants were screened for cytoplasmic islet cel… Show more

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Cited by 81 publications
(67 citation statements)
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“…Evidence supporting this claim included the similarity in biochemical properties of antigens recognized by anti-ganglioside monoclonal antibodies with that of ICA-positive sera [5], and the observation that ICA binding to islet cells is inhibited by glycolipid extracts from human pancreas [6]. Our group recently isolated the first human monoclonal ICA of the lgG-class (MICA 1-6) derived from a patient with newly-diagnosed Type 1 diabetes [7]. MICA 1-6, which represent five different circulating primary B-lymphocyte clones from blood [7,8], were selected by the histochemical ICA test and surprisingly recognized GAD as a target antigen.…”
mentioning
confidence: 99%
“…Evidence supporting this claim included the similarity in biochemical properties of antigens recognized by anti-ganglioside monoclonal antibodies with that of ICA-positive sera [5], and the observation that ICA binding to islet cells is inhibited by glycolipid extracts from human pancreas [6]. Our group recently isolated the first human monoclonal ICA of the lgG-class (MICA 1-6) derived from a patient with newly-diagnosed Type 1 diabetes [7]. MICA 1-6, which represent five different circulating primary B-lymphocyte clones from blood [7,8], were selected by the histochemical ICA test and surprisingly recognized GAD as a target antigen.…”
mentioning
confidence: 99%
“…В литературе [8,11,13] последних лет есть данные, указывающие на достаточно высокую ча-стоту выявления антител к яичниковой ткани в груп-пе больных с аутоиммунным полигландулярным синдромом первого и второго типов. Также извест-но, что идентификация антител к тканям яичников может намного опережать клиническую манифеста-цию заболевания [14,16].…”
Section: обсуждение и выводыunclassified
“…У больных СД1 наиболее изучены ци-топлазматические аутоантитела к островковой ткани β-клеток поджелудочной железы -ICA (islet cell antibodies), но до сих пор остается неясным: почему эти аутоантитела не у всех пациентов являются предше-ственниками болезни [15,16]. В целом ICA является значимым маркером аутоиммунного процесса, на-правленного на островковые клетки, и повышение их титра тесно ассоциируется с прогрессией диабета [7,9].…”
unclassified
“…The ability to identify diabetes-specific GAD65Ab that recognise conformation-dependent epitopes would enhance the value of GAD65Ab as a predictive marker. Several approaches have been used to identify GAD65Ab disease-specific epitopes, including deletion constructs [14], chimeric GAD65/67 molecules [15,16] and naturally occurring GAD65 isoforms [17]. The majority of data suggest that conformation-dependent epitopes are found at the middle and at the C-terminal end of GAD65 [12,15,16,18].…”
mentioning
confidence: 99%
“…The aim of the present study was to determine whether the alteration of a C-terminal GAD65Ab epitope, by substituting GAD67-derived amino acids, defines GAD65Ab with increased diagnostic specificity for Type 1 diabetes. [19]; (ii) mouse monoclonal antibody GAD6, which recognises the C terminus of GAD65 [20]; and (iii) human monoclonal islet cell antibodies (MICA: MICA1, MICA2, MICA3, MICA4 and MICA6) [11,17,21]. Group B included standard sera from ten children (aged 11-16 years) newly diagnosed with Type 1 diabetes, which were obtained at the clinical onset of disease [22].…”
mentioning
confidence: 99%