2002
DOI: 10.1093/emboj/21.7.1723
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Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication

Abstract: Budding yeast Mps1p kinase has been implicated in both the duplication of microtubule-organizing centers and the spindle assembly checkpoint. Here we show that hMps1, the human homolog of yeast Mps1p, is a cell cycle-regulated kinase with maximal activity during M phase. hMps1 localizes to kinetochores and its activity and phosphorylation state increase upon activation of the mitotic checkpoint. By antibody microinjection and siRNA, we demonstrate that hMps1 is required for human cells to undergo checkpoint ar… Show more

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Cited by 238 publications
(297 citation statements)
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“…In this context, this study revealed an unexpected function of TTK in regulating subcellular localization of c-Abl. Whereas TTK is expressed in both the nucleus and the cytoplasm (Stucke et al, 2002), cytoplasmic TTK could be mainly responsible for Thr735 phosphorylation because Thr735 phosphorylation of c-Abl is required for its cytoplasmic sequestration . More importantly, phosphorylation of c-Abl at Thr735 is indispensable for interaction between c-Abl and 14-3-3 .…”
Section: Discussionmentioning
confidence: 99%
“…In this context, this study revealed an unexpected function of TTK in regulating subcellular localization of c-Abl. Whereas TTK is expressed in both the nucleus and the cytoplasm (Stucke et al, 2002), cytoplasmic TTK could be mainly responsible for Thr735 phosphorylation because Thr735 phosphorylation of c-Abl is required for its cytoplasmic sequestration . More importantly, phosphorylation of c-Abl at Thr735 is indispensable for interaction between c-Abl and 14-3-3 .…”
Section: Discussionmentioning
confidence: 99%
“…In addition, suppression of TTK expression in U2OS cells results in defects in spindle checkpoint [18]. Moreover, mass spectrometric identification of molecular composition of CENP-E complex revealed an association between TTKand CENP-E [17].…”
Section: A Model Of Ttk In Spindle Checkpoint Signalingmentioning
confidence: 99%
“…However, several recent studies have raised debates concerning the function and precise localization of TTK in mammalian cells [18,21]. To define more closely the localization of TTK in the mitotic spindle, we carried out immuno-electron microscopic analysis using an affinitypurified antibody (C-19).…”
Section: Ttk Is Associated With Isolated Mitotic Chromosomal Fractionmentioning
confidence: 99%
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“…The Mps1 family of kinases has been described in several other species including mouse (esk/mMps1; Douville et al, 1992), S. cerevisiae (Mps1p; Winey et al, 1991), S. pombe (Mph1p; He et al, 1998), Xenopus laevis (xMps1; Abrieu et al, 2001), zebrafish (mps1/ncp; Poss et al, 2002) and Drosophila (Mps1; Fischer et al, 2004). In budding yeast (S. cerevisiae), this kinase is required for spindle pole duplication and spindle assembly checkpoint, whereas in mammals, although involved in spindle checkpoint, the kinase is thought to be involved in centrosome duplication, although this is debated (Weiss and Winey, 1996;Fisk and Winey, 2001; Stucke et al, 2002;Fisk et al, 2003). During mitosis, Mps1 localizes to kinetochores and is required for proper chromosome alignment and segregation as well as the recruitment of other mitotic checkpoint proteins in the event of unattached chromosome or uneven tension at the kinetochores (Abrieu et al, 2001;Stucke et al, 2004;Vigneron et al, 2004;Jelluma et al, 2008).…”
Section: Introductionmentioning
confidence: 99%