Human Na⁺, K⁺ ‐ATPase genes

Abstract

The existence of a chromosome gene family containing at least one gene and one pseudogene was shown for the Na+,K+-ATPase /?-subunit. A partial structure of the pl-gene was determined, the coding part of which was completely homologous to cDNA of the Na+,K+-ATPase /II-subunit from HeLa cells [8]. The region encoding the putative protein transmembrane domain was shown to be bordered by two introns. The structure of a pseudogene (Bv/) was determined. This pseudogene is processed and contains multiple stop codons. Its homology to the /II-subunit cDNA from HeLa cells is about 88 %.

Na,K-ATPase subunit isoforms in human reticulocytes: Evidence from reverse transcription–PCR for the presence of α1, α3, β2, β3, and γ

“…We confirmed by sequencing that this was due to unspecific binding of the primers to unrelated sequences. In a single experiment, we found a product and initially classified it as ␤1, but further sequencing identified it as the pseudogene ␤ psi (18). We could never repeat this result, and we ascribe it to contamination by genomic DNA in this single experiment.…”

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“…The discovery of a second type of &subunit leads to the conclusion, that a family of P-subunit genes exists. In fact, when analysing a human genomic library using pig kidney cDNA of the P-subunit as a hybridization probe Ushkaryiov et al found at least two different sequences [86]. One sequence presents most probably the gene coding for P-subunit, the isolated fragment contains one exon coding for the putative transmembrane segment and two introns.…”

“…The second sequence highly homologous to the pl gene, is intron free and contains many stop codons, and an insert. Therefore it was proposed that this sequence is a pseudogene belonging to the b-subunit family [86].…”