Human neuroblastoma GOTO cells express CD44 and localize it into lipid rafts upon differentiation into Schwannian cells

Hasegawa, Go; Minami, Natsumi; Kushida, Ai; Inuyama, Hiroki; Koga, Masaki; Wakabayashi, Keiji; Saito, Yuji

doi:10.1016/j.cellbi.2004.09.011

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…The GOTO-derived cell line also expressed myelin-specific proteins to a limited extent [48]. Recently, it has been reported that when GOTO cells differentiate into Schwann-like cells, they express CD44 and localize this molecule to lipid rafts which is characteristic of Schwann cells in culture [65].…”

Section: Goto Human Schwann Cell Line: Origin and Characteristicsmentioning

confidence: 99%

Glial Cell Lines: An Overview

Vries

Boullerne

2010

Neurochem Res

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The importance and essential functions of glial cells in the nervous system are now beginning to be understood and appreciated. Glial cell lines have been instrumental in the elucidation of many of these properties. In this Overview, the origin and properties of most of the existing cell lines for the major glial types: oligodendroglia, astroglia, microglia and Schwann cells, are documented. Particular emphasis is given to the culture conditions for each cell line and the degree to which the line can differentiate in vitro and in vivo. The major molecular markers for each glial cell lines are indicated. Finally, methods by which the glial cell lines have been developed are noted and the future directions of glial cell line research are discussed.

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Section: Goto Human Schwann Cell Line: Origin and Characteristicsmentioning

confidence: 99%

Glial Cell Lines: An Overview

Vries

Boullerne

2010

Neurochem Res

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“…In order to evaluate the capacity of each of the cell lines to differentiate towards glial lineage, BrdU was used as previously described [ 11 , 26 ].…”

Section: Differentiation Studiesmentioning

confidence: 99%

“…Bromodeoxyuridine is a thymidine analog which incorporates into the newly synthesized DNA during the S phase of cell cycle. It has been used as a glial differentiation factor in vitro for NB, inducing cell flattening and increased substrate adhesiveness [ 23 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive characterization of neuroblastoma cell line subtypes reveals bilineage potential similar to neural crest stem cells

et al. 2009

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“…These findings were supported by a study which reported that cultured human neuroblastoma GOTO cells, of which a cell adhesion molecule CD44 expression is usually suppressed, could be induced to differentiate into Schwannian cells and neuronal cells in the presence of 5-bromo-2’-deoxyuridine (BrdU) and by serum depletion respectively. These GOTO cells differentiated into Schwannian cells, specifically expressed CD44 glycoprotein, while this molecule remained suppressed in cells differentiated into neuronal cells which suggests that CD44 might play an important role in GOTO cells differentiation into Schwannian cells [36] and thus in tumor evolution into maturing subtypes.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of CD44s in human neuroblastic tumors: Moroccan experience and highlights on current data

et al. 2013

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BackgroundPeripheral neuroblastic tumors (pNTs), including neuroblastoma (NB), ganglioneuroblastoma (GNB) and ganglioneuroma (GN), are extremely heterogeneous pediatric tumors responsible for 15 % of childhood cancer death. The aim of the study was to evaluate the expression of CD44s (‘s’: standard form) cell adhesion molecule by comparison with other specific prognostic markers.MethodsAn immunohistochemical profile of 32 formalin-fixed paraffin-embedded pNTs tissues, diagnosed between January 2007 and December 2010, was carried out.ResultsOur results have demonstrated the association of CD44s negative pNTs cells to lack of differentiation and tumour progression. A significant association between absence of CD44s expression and metastasis in human pNTs has been reported. We also found that expression of CD44s defines subgroups of patients without MYCN amplification as evidenced by its association with low INSS stages, absence of metastasis and favorable Shimada histology.DiscussionThese findings support the thesis of the role of CD44s glycoprotein in the invasive growth potential of neoplastic cells and suggest that its expression could be taken into consideration in the therapeutic approaches targeting metastases.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1034403150888863RésuméIntroductionles tumeurs neuroblastiques périphériques (TNPs), comprenant le neuroblastome (NB), le ganglioneuroblastome (GNB) et le ganglioneurome (GN), sont des tumeurs pédiatriques extrêmement hétérogènes responsables de 15% des décès par cancer chez les enfants. Le but de cette étude était d’évaluer l’expression de la molécule d’adhésion cellulaire CD44s (‘s’: pour standard) par rapport à d’autres facteurs pronostiques spécifiques.MéthodesUn profil immunohistochimique de 32 TNPs fixées au formol et incluses en paraffine, diagnostiquées entre Janvier 2007 et Décembre 2010, a été réalisé.RésultatsNos résultats ont mis en évidence l’association des TNPs n’exprimant pas le CD44s avec une perte de différenciation et une progression tumorale et nous avons rapporté une association significative entre l’absence d’expression du CD44s et la présence de métastases. Nous avons également constaté que l’expression du CD44s définit des sous-groupes de patients dans les tumeurs n’amplifiant pas le MYCN, comme en témoigne son association avec les stades INSS bas, l’absence de métastases et l’histologie favorable de Shimada.DiscussionCes résultats appuient l’hypothèse du rôle de la glycoprotéine CD44s dans le potentiel de croissance invasive des cellules néoplasiques et suggèrent que son expression pourrait être prise en considération dans des voies thérapeutiques ciblant les métastases.

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Human neuroblastoma GOTO cells express CD44 and localize it into lipid rafts upon differentiation into Schwannian cells

Cited by 5 publications

References 27 publications

Glial Cell Lines: An Overview

Glial Cell Lines: An Overview

Comprehensive characterization of neuroblastoma cell line subtypes reveals bilineage potential similar to neural crest stem cells

Immunohistochemical expression of CD44s in human neuroblastic tumors: Moroccan experience and highlights on current data

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