Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent

Hauck, Barbara; Philipp, Alois; Eckstein, Reinhold; Ott, Sabine; Zimmermann, R.; Dengler, T.; Zingsem, J.

doi:10.1111/j.1399-0039.2011.01779.x

Cited by 27 publications

(49 citation statements)

References 19 publications

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“…To date, all the results have been concordant with pre-typed controls. From the 1,520 platelet donor samples of the Basel and Zurich areas, HPA allele frequencies were well comparable to data previously reported for the Austrian population [62] while HNA allele frequencies are proving to be similar to those observed in Germany [63].…”

Section: Maldi-tof Ms In Transfusion Medicinesupporting

confidence: 73%

Molecular Diagnostics in Transfusion Medicine: MALDI-TOF MS for Blood Bankers- The Alternative Approach for Genotyping

Molan¹

2014

IOSRJPBS

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Although matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry (MALDI-TOF MS) has been previously reported for high throughput blood group genotyping, those reports are limited to only a few blood group systems. This report is timely as promising preliminary results have recently been published from a large cooperative Swiss-German project that aimed to employ MALDI-TOF MS for the molecular detection of the blood groups

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Section: Maldi-tof Ms In Transfusion Medicinesupporting

confidence: 73%

Molecular Diagnostics in Transfusion Medicine: MALDI-TOF MS for Blood Bankers- The Alternative Approach for Genotyping

Molan¹

2014

IOSRJPBS

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“…The frequency of the SNP indicative of the SLC44A2*1 and SLC44A2*2 alleles is known for Caucasian, African American, Han Chinese, Japanese, Tunisian, Brazilian, Thai, and other populations . Chu and colleagues applied an algorithmic approach to predict HNA antigens from whole genome sequencing (WGS) data.…”

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confidence: 99%

Full‐length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen‐3

et al. 2015

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Background HNA-3a alloantibodies can cause severe transfusion-related acute lung injury (TRALI). The frequency of the single nucleotide polymorphisms (SNPs) indicative of the two clinically relevant HNA-3a/b antigens are known in many populations. In the present study, we determined the full length nucleotide sequence of common SLC44A2 alleles encoding the choline transporter-like protein-2 (CTL2) that harbors HNA-3a/b antigens. Study design and methods A method was devised to determine the full length coding sequence and adjacent intron sequences from genomic DNA by 8 polymerase chain reaction (PCR) amplifications covering all 22 SLC44A2 exons. Samples from 200 African American, 96 Caucasian, 2 Hispanic and 4 Asian blood donors were analyzed. We developed a decision tree to determine alleles (confirmed haplotypes) from the genotype data. Results A total of 10 SNPs were detected in the SLC44A2 coding sequence. The non-coding sequences harbored an additional 28 SNPs (1 in the 5’-untranslated region (UTR); 23 in the introns; and 4 in the 3’-UTR). No SNP indicative of a non-functional allele was detected. The nucleotide sequences for 30 SLC44A2 alleles (haplotypes) were confirmed. There may be 66 haplotypes among the 604 chromosomes screened. Conclusions We found 38 SNPs, including 1 novel SNP, in 8192 nucleotides covering the coding sequence of the SLC44A2 gene among 302 blood donors. Population frequencies of these SNPs were established for African Americans and Caucasians. Because alleles encoding HNA-3b are more common than non-functional SLC44A2 alleles, we confirmed our previous postulate that African American donors are less likely to form HNA-3a antibodies compared to Caucasians.

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“…The FCGR3B*1 and FCGR3B*2 allele frequencies of the northern Thais did not differ from those of the Taiwanese and Japanese populations [9, 14]. Interestingly, the FCGR3B*1 and FCGR3B*2 allele frequencies were significantly different from those observed in the central Thai, German, Turkish, Tunisian, and Korean populations ( P <0.01) [16, 20, 21]. …”

Section: Resultsmentioning

confidence: 67%

“…Moreover, the FCGR3B locus was in Hardy-Weinberg equilibrium in Thai populations. The genotype frequency distribution of the central Thai population was also compared with that of the northern Thai population and other studies previously reported [9, 14, 16, 20, 21]. The FCGR3B*1 and FCGR3B*2 allele frequencies of the northern Thais did not differ from those of the Taiwanese and Japanese populations [9, 14].…”

Section: Resultsmentioning

confidence: 75%

Frequency of FCGR3B Alleles in Thai Blood Donors

et al. 2013

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BackgroundHuman neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations.MethodsEight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique.ResultsThe frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the FCGR3B*2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations.ConclusionsFCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection.

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Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent

Cited by 27 publications

References 19 publications

Molecular Diagnostics in Transfusion Medicine: MALDI-TOF MS for Blood Bankers- The Alternative Approach for Genotyping

Molecular Diagnostics in Transfusion Medicine: MALDI-TOF MS for Blood Bankers- The Alternative Approach for Genotyping

Full‐length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen‐3

Frequency of FCGR3B Alleles in Thai Blood Donors

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