Human neutrophil antigens: Nature, clinical significance and detection

Browne, Tom; Dearman, Rebecca J.; Poles, Anthony

doi:10.1111/iji.12514

Cited by 18 publications

(27 citation statements)

References 81 publications

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“…One of the most interesting changes was in ITGAL TUBB3, ADRB2, SLP1, and CCL19 CTSG, with slightly lower expression of the minimal lesion and active lesion and significantly higher than the chronic and mixed lesion. ITGAL is involved in various immune phenomena including leukocyte-endothelial cell interactions, cytotoxic T cell-mediated killing, and antibody-dependent killing by granulocytes and monocytes [ 37 ]. CCL19 plays an important role in the trafficking of T cells in the thymus and T cell and B cell migration to secondary lymphoid organs [ 38 ]; CTSG regulates inflammation, activating matrix metalloproteinases (MMPs) and coagulation [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The Diagnostic and Predictive Significance of Immune-Related Genes and Immune Characteristics in the Occurrence and Progression of IgA Nephropathy

Qing

Song

et al. 2022

Journal of Immunology Research

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Objective. To investigate the potential diagnostic and predictive significance of immune-related genes in IgA nephropathy (IgAN) and discover the abnormal glomerular inflammation in IgAN. Methods. GSE116626 was used as a training set to identify different immune-related genes (DIRGs) and establish machine learning models for the diagnosis of IgAN; then, a nomogram model was generated based on GSE116626, and GSE115857 was used as a test set to evaluate its clinical value. Short Time-Series Expression Miner (STEM) analysis was also performed to explore the changing trend of DIRGs with the progression of IgAN lesions. GSE141344 was used with DIRGs to establish the ceRNA network associated with IgAN progression. Finally, ssGSEA analysis was performed on the GSE141295 dataset to discover the abnormal inflammation in IgAN. Results. Machine learning (ML) performed excellently in diagnosing IgAN using six DIRGs. A nomogram model was constructed to predict IgAN based on the six DIRGs. Three trends related to IgAN lesions were identified using STEM analysis. A ceRNA network associated with IgAN progression which contained 8 miRNAs, 14 lncRNAs, and 3 mRNAs was established. A higher macrophage ratio and lower CD4+ T cell ratio in IgAN compared to controls were observed, and the correlation between macrophages and monocytes in the glomeruli of IgAN patients was inverse compared to controls. Conclusion. This study reveals the diagnostic and predictive significance of DIRGs in IgAN and finds that the imbalance between macrophages and CD4+ immune cells may be an important pathomechanism of IgAN. These results provide potential directions for the treatment and prevention of IgAN.

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Section: Discussionmentioning

confidence: 99%

The Diagnostic and Predictive Significance of Immune-Related Genes and Immune Characteristics in the Occurrence and Progression of IgA Nephropathy

Qing

Song

et al. 2022

Journal of Immunology Research

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“…Human neutrophil antigens (HNAs) are glycoproteins expressed on the human neutrophils surface [ 1 ]. On human neutrophils, there are five antigenic systems (from HNA-1 to HNA-5) that consist of fourteen alleles located on the human neutrophil Fc gamma-receptor IIIb (Fc Y RIIIb) (CD16), CD177, choline transporter-like protein 2 (CTL2), Mac1 (CD11b/CD18), and LFA1 (CD11a/CD18), respectively [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors

Esmaeili

Bayat

Alirezaee

et al. 2022

Journal of Immunology Research

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Objective. Human neutrophil antigens (HNAs) can be targeted by HNA-allo antibodies and cause a variety of clinical conditions such as transfusion-related acute lung injury (TRALI) and neonatal alloimmune neutropenia (NAIN). The current study is aimed at identifying the genotype and allele frequencies of HNAs in Iranian blood donors. Methods. A total of 150 blood samples were obtained from healthy blood donors. HNA-1, HNA-3, HNA-4, and HNA-5 were genotyped, using the polymerase chain reaction sequence-specific primer (PCR-SSP) technique. The expression of the HNA-2 antigen on the neutrophil surface was evaluated by flow cytometry. Results. The allele frequencies of FCGR3B ∗ 1 (encoding HNA-1a), FCGR3B ∗ 2 (encoding HNA-1b), and FCGR3B ∗ 3 (encoding HNA-1c) were 0.34, 0.63, and 0.03, respectively. For HNA-3, the allele frequencies for SLC44A2 ∗ 1 (encoding HNA-3a) and SLC44A2 ∗ 2 (encoding HNA-3b) were 0.63 and 0.37, respectively. The frequencies of ITGAM ∗ 1 (encoding HNA-4a) and ITGAM ∗ 2 (encoding HNA-4b) alleles were 0.85 and 0.15, respectively. Furthermore, the frequencies of ITGAL ∗ 1 (encoding HNA-5a) and ITGAL ∗ 2 (encoding HNA-5b) alleles were 0.72 and 0.28, respectively. In the studied population, HNA-2 antigen was present on the neutrophil surface in 97.3% of the individuals, while no detectable HNA-2 expression was observed in 2.7% of the individuals. However, no significant difference in HNA-2 expression between different age groups was found. Conclusion. The present study provides the first report of the HNA allele and genotype frequencies among the Iranian population. All HNAs (HNA-1 to HNA-5) were typed using the PCR-SSP and flow cytometer. In the current cohort study, the determined HNA allele frequencies were similar to the previous reports from British, German, and Danish populations. Considering the presence of different Iranian ethnic groups, further studies with a larger sample size are needed to draw a total picture for HNA allele frequencies.

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“…Granulocytes constitute the major proportion of human leukocytes and are an inevitable part of the innate immune system. As a significant majority (~99%) of the granulocyte population primarily comprises of neutrophils, a family of glycoprotein epitopes expressed on granulocytes have been collectively named as the human neutrophil antigens (HNAs) (Browne et al, 2021), (Flesch & Reil, 2018). Based on the serologically defined epitopes, these antigens are classified into five major groups/systems namely HNA-1 to HNA-5.…”

Section: Introductionmentioning

confidence: 99%

Genetic epidemiology of human neutrophil antigen variants suggest significant global variability

Rophina

Scaria

2022

Preprint

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Human neutrophil antigens possess significant clinical implications especially in the fields of transfusion and transplantation medicine. Efforts to estimate the prevalence of genetic variations underpinning the antigenic expression are emerging. However, there lacks a precise capture of the global frequency profiles. Our article emphasizes the potential utility of maintaining an organized online repository of evidence on neutrophil antigen associated genetic variants from published literature and reports. This in our opinion, is an emerging area and would significantly benefit from the awareness and understanding of population-level diversities.

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Human neutrophil antigens: Nature, clinical significance and detection

Cited by 18 publications

References 81 publications

The Diagnostic and Predictive Significance of Immune-Related Genes and Immune Characteristics in the Occurrence and Progression of IgA Nephropathy

The Diagnostic and Predictive Significance of Immune-Related Genes and Immune Characteristics in the Occurrence and Progression of IgA Nephropathy

Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors

Genetic epidemiology of human neutrophil antigen variants suggest significant global variability

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