Human Neutrophil Granule Cationic Protein CAP37 is a Specific Macrophage Chemotaxin that Shares Homology with Inflammatory Proteinases

Characterization of recombinant human HBP/CAP37/azurocidin, a pleiotropic mediator of inflammation‐enhancing LPS‐induced cytokine release from monocytes

“…Based on the human HBP amino acid sequence [3] and the CAP37/ azurocidin cDNA sequence [8,9] a 770 bp BamHI HindlII fragment was constructed by PCR technology by usage of a human bone marrow cDNA library (Clontech). This fragment contains the entire coding region of HBP, including a 19-residue signal peptide, a 7 amino acid pro-peptide, a mature part of 222 amino acids, and a 3 amino acid C-terminal extension.…”

“…The cDNA for HBP has been cloned and characterized [8,9], and in order to produce the protein by recombinant techniques we have tried different expression systems (yeast and mammalian cells) with very limited success. By usage of the baculovirus/insect cell system we report in the present *Corresponding author.…”

CAP37 Activation of PKC Promotes Human Corneal Epithelial Cell Chemotaxis

“…[5][6][7][8] CAP37 regulates monocyte, macrophage, and microglial functions by promoting migration, phagocytosis, and activation of these cells to produce proinflammatory cytokines. 3,9,10 Furthermore, CAP37 upregulates adhesion molecules on endothelial, smooth muscle, and corneal epithelial cells. 6,8,11 Its ability to upregulate adhesion molecules and to mediate migration and proliferation of human corneal epithelial cells (HCECs) in vitro led us to postulate that CAP37 may have an important role in corneal wound healing.…”

Novel Therapies Based on Cationic Antimicrobial Peptides

“…The antibacterial domain of BPI was delineated to a 25 kDa amino acid fragment and recombinant BPI 23 are both active in vitro and in vivo against Gram negative bacteria [47,48]. Small synthetic peptides based on the CAP37 molecule [49,50] were screened for antimicrobial activity and the primary antimicrobial domain was delineated to a 25 amino acid peptide that comprises amino acids 20 through 44 of the native CAP37 protein, CAP37 20-44 [46].…”