Human non-Hodgkin's malignant lymphomas serially transplanted in nude mice conditioned with whole-body irradiation

Igarashi, Tsuyoshi; Oka, Kuniyuki; Miyamoto, Tadaaki

doi:10.1038/bjc.1989.71

Cited by 7 publications

(1 citation statement)

References 24 publications

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“…Two days before transplantation, all of the mice were irradiated with X-ray at a dose of 4 J/kg [10]. POS cells (1 × 10 7 /mouse) or CHOS cells (1 × 10 7 /mouse) were subcutaneously injected into 15 mice and 12 mice, respectively.…”

Section: Methodsmentioning

confidence: 99%

Anti-Tumor Effect of Adenoviral Vector-Mediated p53 Gene Transfer on the Growth of Canine Osteosarcoma Xenografts in Nude Mice

Kanaya

Yazawa

Goto‐Koshino

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. We evaluated the anti-tumor effect of adenoviral vector-mediated p53 gene therapy on the growth of canine osteosarcoma xenografts formed in nude mice. Nude mice were subcutaneously transplanted with cells of 2 P53 mutant canine osteosarcoma cell lines, POS and CHOS. The osteosarcoma xenografts were injected with either an adenoviral vector that expresses canine wild-type P53 (AxCA-cp53) or LacZ (AxCA-LacZ). Tumor growth was significantly inhibited in the xenografts injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS during the observation period of 27 days. An increase of the amount of p21 WAF1/CDKN1A mRNA, and the number of apoptotic cells was shown in the tumors injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS. The present study revealed that the adenoviral vector-mediated p53 gene transfer had an anti-tumor effect in canine osteosarcoma xenografts formed in nude mice.

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Section: Methodsmentioning

confidence: 99%

Anti-Tumor Effect of Adenoviral Vector-Mediated p53 Gene Transfer on the Growth of Canine Osteosarcoma Xenografts in Nude Mice

Kanaya

Yazawa

Goto‐Koshino

et al. 2011

The Journal of Veterinary Medical Science

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Engraftment of human non-hodgkin lymphomas in mice with severe combined immunodeficiency

Itoh¹,

Shiota²,

Takanashi³

et al. 1993

Cancer

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Background. Malignant non‐Hodgkin lymphoma (NHL) is one of the most difficult neoplasms to transplant into nude mice. Mice with severe combined immunodeficiency (SCID) accept various human cancers much more efficiently than do nude mice. The authors investigated whether SCID mice could be used as convenient hosts in which to grow human NHL in vivo. Methods. Fifty NHL specimens were engrafted into SCID mice. The original specimens and the tumors that developed in SCID mice were studied immunohistologically and by Southern blot analysis to clarify their clonal identity and to determine if they were Epstein–Barr virus (EBV)‐transformed B cell proliferations. Results. SCID tumors developed from 23 of 50 NHL specimens. Ten tumors were identical immunophenotypically and, partly, genotypically to the original NHL, showing that the original NHL grew in the SCID mice. B‐cell NHL rather than T‐cell NHL and high‐grade rather than low‐grade malignancy groups were much more easily heterotransplanted. Most of the heterotransplanted NHL were maintained by successive transplantation. In two other SCID tumors, the original NHL clones and a newly developed B‐cell clone coexisted. The remaining 11 SCID tumors were composed of newly developed clones. The latter 13 tumors were shown to be human cells of B‐cell lineage bearing EBV latent proteins–‐latent membrane protein 1 and EB nuclear antigen 2–‐suggesting that they originated from EBV‐infected B‐cells that were present in the original tumor tissues. Conclusion. SCID mice accept human NHL far more efficiently than do nude mice. However, frequent occurrence of spontaneous EBV‐associated B‐cell proliferation must be kept in mind.

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Efficacy of AZT therapy in reducing p24 antigen burden in a modified SCID mouse model of HIV infection

Alder

Clement

1995

Antiviral Research

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Human non-Hodgkin's malignant lymphomas serially transplanted in nude mice conditioned with whole-body irradiation

Cited by 7 publications

References 24 publications

Anti-Tumor Effect of Adenoviral Vector-Mediated p53 Gene Transfer on the Growth of Canine Osteosarcoma Xenografts in Nude Mice

Anti-Tumor Effect of Adenoviral Vector-Mediated p53 Gene Transfer on the Growth of Canine Osteosarcoma Xenografts in Nude Mice

Engraftment of human non-hodgkin lymphomas in mice with severe combined immunodeficiency

Efficacy of AZT therapy in reducing p24 antigen burden in a modified SCID mouse model of HIV infection

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