Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro

Abstract

Mesenchymal stem cells (MSCs) have been reported to participate in the formation of supportive tumor stroma. The abilities of proliferation and invasion of human epithelial ovarian cancer (EOC) cells were significantly enhanced when indirectly cocultured with human omental adipose-derived MSCs (O-ADSCs) in vitro. However, the underlying mechanisms remain poorly understood. In this study, EOC cells were cultured with conditioned medium (CM) from O-ADSCs (O-ADSC), and the effect of O-ADSC CM on the proteomic profile of EOC cells was assessed by two-dimensional gel electrophoresis (2-DE), followed by liquid chromatography and tandem mass spectrometry. The 2-DE assays revealed a global increase in protein expression in the EOC cells treated with CM. Nine proteins were identified from 11 selected protein spots with differential expression after treatment with CM from O-ADSCs. All the nine proteins have been linked to carcinoma and apoptosis, and the migration ability of tumor cells can be regulated by these proteins. Moreover, the upregulation of prohibitin and serine/arginine-rich splicing factor 1 in EOC cells treated with CM was further confirmed by quantitative real-time polymerase chain reaction. These results suggest that O-ADSCs affect the proteomic profile of EOC cells via paracrine mechanism in favor of EOC progression.

The inhibition of cordycepin on cancer stemness in TGF-beta induced chemo-resistant ovarian cancer cell

“…In addition, 100 μM of Cd treatment potentially reduced stemness of SKOV-3 cancer cells caused by TGF-beta. Matrix metalloproteinases (MMPs) including MMP-2, MMP-7, and MMP-9 are known to promote tumor progression and cancer stemness [ 21 ]. We found that TGF-beta treatment significantly increased the levels of MMP-2, MMP-7, and MMP-9 while Cd treatment decreased MMPs levels (Figure 3 ).…”

Epigenetic therapy can inhibit growth of ovarian cancer cells and reverse chemoresistant properties acquired from metastatic omentum

“…Omental microenvironment serves as a major site for tumor metastases, proliferation, and chemotherapy response in EOC. It has now been shown that omentum acts via secretion of paracrine growth factors although the underlying mechanisms are not fully understood . Pathologic review of notable morphological differences in OASCs from the present study possibly reflected the effect that tumor cells have on omental microenvironment and in turn influenced their secretomic expression.…”

Revisiting ovarian cancer microenvironment: a friend or a foe?

“…Both proliferation and invasion of human EOC cells are remarkably enhanced upon co-culture with omental adipose-derived MSCs (O-ADSCs) in vitro , and a recent study revealed a global increase in protein expression in the EOC cells treated with conditioned media (CM) from O-ADSC (Zhang et al, 2017 ). Specifically, nine proteins were identified with differential expression after CM treatment, which are linked to carcinogenesis, apoptosis and migration of cancer cells, suggest that O-ADSCs alter the proteomic profile of EOC cells via paracrine mechanism in favor of EOC progression (Zhang et al, 2017 ).…”