Human omental adipose‐derived stem cells from donors with different body mass index had similar effects on proliferation and migration of endometrial cancer cells in vitro

Abstract

Aim: This study aimed to confirm whether human omental adipose-derived stem cells (O-ASC) from donors with varying body mass index (BMI, calculated in kg/m 2 ) exert different effects on proliferation and migration of endometrial cancer (EC) cells. Methods: Omental adipose-derived stem cells were isolated from the omental adipose tissues of eight patients who were diagnosed with EC aged from 35 to 56 years. According to the patients' BMI, the O-ASC was divided into two groups: obesity group (BMI ≥ 30) and normal group (18.5 < BMI ≤ 24.9). A broad spectrum cytokine antibody array was used to measure 62 paracrine cytokines secreted by all the O-ASC. MTS assays, direct and indirect co-culture assays were used to assess the effects of O-ASC on proliferation and migration of Hec-1A (estrogen receptor−/progesterone receptor−) and Ishikawa (estrogen receptor+/progesterone receptor+) EC cells. Results: Eight samples of O-ASC were successfully isolated including four samples in obesity group and four in normal group. All the O-ASC displayed typical characteristics of mesenchymal stem cells and possessed similar secretory functions as 26 cytokines were identified in condition medium of O-ASC based on cytokine antibody array. The proliferation of Ishikawa cells was gently stimulated by O-ASC from the two groups (P < 0.05) whereas without any effects on Hec-1A cells. Both horizontal and vertical migrations of EC cells were promoted by O-ASC (P < 0.01). However, there were no statistical differences between the two groups. Conclusion: Human O-ASC could influence the proliferation and migration of EC cells in vitro but the effects were not modified by donors' BMI.

Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs

“…De Girolamo et al reported lower expression of CD54 and CD34 in obASCs than in lnASCs [ 17 ]. On the other hand, it has been reported that obesity of the donor does not affect the CD marker expression of ASCs [ 26 , 44 , 45 ]. In the current study, ASC from all donors showed a characteristic immunophenotype of MSCs.…”

Emerging role of mesenchymal stromal cells in gynecologic cancer therapy

“…Direct co-cultured experiments found that O-AT-MSC could fuse with endometrial cancer Ishikawa and HEC-1-A cells to spontaneously form hybrid tumor spheres, leading to the enhanced cancer mobility and heterogeneity. According to body mass index (BMI), Li et al [ 101 ] further obtained the O-AT-MSC from endometrial cancer patients with different BMI (BMI ≥ 30 and 18.5 < BMI < 24.9). Although the promotive effects of the O-AT-MSC on the proliferation and migration of HEC-1-A and Ishikawa cells were comparable, no significant differences were observed between the two groups.…”

Ion Channels and Personalized Medicine in Gynecological Cancers

“…However, the regulatory mechanisms involving CFTR in EC may be opposite in vitro compared to the in vivo conditions. Additionally, in Ishikawa cells, blockade of volume-activated chloride channels (VACC) with 5-nitro-2-(3-phenylpropyl-amino)-benzoate (NPPB) inhibited invasion and regulatory volume decrease in a dose-dependent manner [92]. Voltage-dependent anion channel-1 (VDAC1) and VDAC3 are overexpressed in EC in comparison to normal tissues, and higher expression of all VDAC or protein expression of VDAC2 was associated with poor prognosis [93].…”