Human oropharynx as natural reservoir of Streptobacillus hongkongensis

Lau, Susanna K. P.; Chan, Jasper Fuk Woo; Tsang, Chi‐Ching; Chan, Sau Man; Ho, Man Ling; Que, Tak Lun; Lau, Yu Lung; Woo, Patrick C. Y.

doi:10.1038/srep24419

“…According to the author Lau et al (21), Streptobacillus hongkongensis is a novel bacterial species that permanently found in human oropharynx and there might be more other…”

Section: Resultsmentioning

confidence: 99%

Mucosa-associated Cultivable Aerobic Gut Bacterial Microbiota among Colorectal Cancer Patients Attending at the Referral Hospitals of Amhara Regional State, Ethiopia

Siraj

¹

,

Byadgelegn

²

,

Yassin

³

et al. 2020

Preprint

0

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Background: Colorectal cancer is one of the top ten cancer death in the world. Despite an increased prevalence of colorectal cancer has been documented from developing countries, reports on gut microbiota among colorectal cancer patients are none especially in Ethiopia. Therefore, the current study evaluated cultivable aerobic bacterial distributions among malignant tissue of colorectal cancer and its adjacent normal biopsies. Methods: Fifteen CRC patients who were undergoing colorectal cancer resection surgery during April 2017 to February 2018 at Felege Hiwot Referral and University of Gondar Teaching Hospitals were included. Biopsy specimens were taken from malignant and its adjacent normal tissues. Bacterial cultivation, quantification and characterization of saline washed biopsies were performed under aerobic and candle jar conditions. Differences in bacterial microbiota compositions between malignant and normal tissue biopsies were evaluated and analyzed using Microsoft excel 2010 and GraphPad Prism5 statistical software. Results: Fifteen CRC patients were participated with a mean age of 53.8 ± 10.8 years old and majorities (73.3%) of patients were in between the age groups of 40 and 60 years old. The mean ± SD bacterial microbiota of malignant biopsies (3.2x10 5 ± 1.6x10 5 CFU/ml) was significantly fewer than that of adjacent normal tissue biopsies (4.0x10 5 ± 2.2x10 5 CFU/ml). This dysbacteriosis is positively correlated with the occurrence of CRC (p=0.019). Proteobacteria (55.6%), Firmicutes (33.3%) and Fusobacteria (11.1%) were the most frequently isolated phyla from non-malignant biopsies while only Proteobacteria (58.8%) and Firmicutes (41.2%) were from malignant ones. Family level differences were observed among phyla (Firmicutes and Proteobacteria) isolated from the study participants. For instance, the relative abundance of family Bacillaceae from malignant (26%) was lower than the normal biopsies (39%). On other hand, family Enterobacteriaceae was twice more abundant in malignant tissues (45%) than in its matched normal tissues (23%). Furthermore, the family Enterococcaceae (14%) of family Firmicutes was solely isolated from malignant tissue biopsies. Conclusion: The overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition differences and pathogenesis of CRC in Ethiopian settings.

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“…Differential abundance testing of bacterial communities based on nicotine dependence scores revealed a relative abundance of Streptobacillus hongkongensis among more nicotine dependent smokers (high Fagerström score). Previous studies reported the isolation of S. hongkongensis from patients with quinsy, pneumonia, and septic arthritis [34,35], which was later reported as part of the human oropharynx natural reservoir [36]. Increased risk of developing serious respiratory illnesses might be partly attributed to more nicotine dependent smokers.…”

Section: Discussionmentioning

confidence: 99%

Revealing oral microbiota composition and functionality associated with heavy cigarette smoking.

Bataineh

¹

,

Dash

²

,

Elkhazendar

³

et al. 2020

Preprint

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Background: Heavy tobacco smoking, a hallmark feature of lung cancer, is drastically predominant in Middle Eastern populations. The precise links between nicotine dependence and the functional contribution of the oral microbiota remain unknown in these populations.Methods: We evaluated the composition and functional capabilities of oral microbiota with relation to cigarette smoking in 105 adults through shotgun metagenomics using buccal swabs. Results: The oral microbiota composition in our study subjects was dominated by the phyla Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, in addition to the genera Prevotella and Veillonella, similar to previously described westernized cohorts. Furthermore, the smoker's oral microbiota represented a significant abundance of Veillonella dispar, Leptotrichia spp., and Prevotella pleuritidis when compared to non-smokers. Within the smoking groups, differential relative abundance testing unveiled relative abundance of Streptobacillus hongkongensis, Fusobacterium massiliense, Prevotella bivia in high nicotine dependent compared to low nicotine dependent profiles based on Fagerström Test for Nicotine Dependence. Functional profiling showed marked differences between smokers and non-smokers. Smokers exhibited an enrichment of Tricarballylate utilization and Lactate racemization when compared to the non-smokers. According to their nicotine dependence, enrichment of Xanthosine utilization, p-Aminobenzoyl-Glutamate utilization, and multidrug efflux pump in Campylobacter jejuni biosynthesis modules were detected in the high nicotine dependent group. Conclusions: These compositional and functional differences may provide critical insight on how variations in the oral microbiota could predispose to respiratory illnesses and smoke cessation relapse in cigarette smokers. In particular, the observed enrichment of Fusobacterium and Prevotella in the oral microbiota possibly suggests an intriguing linkage to gut and lung cancers.

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“…Differential abundance testing of bacterial communities based on nicotine dependence scores revealed a relative abundance of Streptobacillus hongkongensis among more nicotine dependent smokers (high Fagerström score). Previous studies reported the isolation of S. hongkongensis from patients with quinsy, pneumonia, and septic arthritis [36,37], which was later reported as part of the human oropharynx natural reservoir [38]. Increased risk of developing serious respiratory illnesses might be partly attributed to more nicotine dependent smokers.…”

Section: Discussionmentioning

confidence: 99%

Revealing oral microbiota composition and functionality associated with heavy cigarette smoking.

Bataineh

¹

,

Dash

²

,

Elkhazendar

³

et al. 2020

Preprint

1

0

View full text Add to dashboard Cite

Background: Heavy tobacco smoking, a hallmark feature of lung cancer, is drastically predominant in Middle Eastern populations. The precise links between nicotine dependence and the functional contribution of the oral microbiota remain unknown in these populations.Methods: We evaluated the composition and functional capabilities of oral microbiota with relation to cigarette smoking in 105 adults through shotgun metagenomics using buccal swabs. Results: The oral microbiota composition in our study subjects was dominated by the phyla Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, in addition to the genera Prevotella and Veillonella, similar to previously described westernized cohorts. Furthermore, the smoker's oral microbiota represented a significant abundance of Veillonella dispar, Leptotrichia spp., and Prevotella pleuritidis when compared to non-smokers. Within the smoking groups, differential relative abundance testing unveiled relative abundance of Streptobacillus hongkongensis, Fusobacterium massiliense, Prevotella bivia in high nicotine dependent compared to low nicotine dependent profiles based on Fagerström Test for Nicotine Dependence. Functional profiling showed marked differences between smokers and non-smokers. Smokers exhibited an enrichment of Tricarballylate utilization and Lactate racemization when compared to the non-smokers. According to their nicotine dependence, enrichment of Xanthosine utilization, p-Aminobenzoyl-Glutamate utilization, and multidrug efflux pump in Campylobacter jejuni biosynthesis modules were detected in the high nicotine dependent group. Conclusions: These compositional and functional differences may provide critical insight on how variations in the oral microbiota could predispose to respiratory illnesses and smoke cessation relapse in cigarette smokers. In particular, the observed enrichment of Fusobacterium and Prevotella in the oral microbiota possibly suggests an intriguing linkage to gut and lung cancers.

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Human oropharynx as natural reservoir of Streptobacillus hongkongensis

Cited by 18 publications

References 40 publications

Mucosa-associated Cultivable Aerobic Gut Bacterial Microbiota among Colorectal Cancer Patients Attending at the Referral Hospitals of Amhara Regional State, Ethiopia

Mucosa-associated Cultivable Aerobic Gut Bacterial Microbiota among Colorectal Cancer Patients Attending at the Referral Hospitals of Amhara Regional State, Ethiopia

Revealing oral microbiota composition and functionality associated with heavy cigarette smoking.

Revealing oral microbiota composition and functionality associated with heavy cigarette smoking.

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