Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos

Xu, Jianbing; Cheung, Tak-Ming; Chan, S; Ho, Pak‐Chung; Yeung, William S.B.

doi:10.1016/s0015-0282(00)01618-6

Cited by 40 publications

(30 citation statements)

References 28 publications

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“…Mouse twocell embryos do not develop into blastocysts when exposed to BMP4, although BMP4 induces expression of Cdx2 in the ICM of existing blastocysts (86). On the other hand, human oviductal cells express high levels of Noggin (87) and cause blastocyst formation in mouse embryos (88). Trophoblasts can be derived from mESCs using Noggin (36).…”

Section: Discussionmentioning

confidence: 99%

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

Sarkar

Randall

Collier

et al. 2015

Journal of Biological Chemistry

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Background: Specification of terminal fate in trophoblasts derived from human embryonic stem cells is not understood. Results: Inhibition of activin/nodal signaling triggers extravillous fate, but loss of inhibition causes syncytial fate. Conclusion: Activin/nodal signaling switches the terminal fate of trophoblasts. Significance: We provide a model system that allows for targeted derivation of extravillous trophoblasts and syncytiotrophoblasts.

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Section: Discussionmentioning

confidence: 99%

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

Sarkar

Randall

Collier

et al. 2015

Journal of Biological Chemistry

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“…The overall low levels of apoptosis reflect positively on the quality of embryos and the basal medium used in this study. Apoptotic indices of freshly isolated embryos in an un-supplemented control medium has ranged from (1.5 to 32%) in other published studies, depending on the animal species, culture medium and embryo stage [4,5,10,12,28,29,44,49].…”

Section: Discussionmentioning

confidence: 99%

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Desai

Kattal

AbdelHafez

et al. 2007

J Assist Reprod Genet

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“…such as: higher and faster cleavage (Bongso et al, 1989), improved morphological appearance/ grade (Wiemer et al, 1989), increase of the average number of blastomeres (Smith et al, 1992), improved post-thaw blastomere survival of cryopreserved co-cultured embryos (Tucker et al, 1995), reduced apoptosis (Xu et al, 2000), higher blastocyst rate (Joo et al, 2001), facilitated hatching (Ellington et al, 1990), lower fragmentation rates, improved pregnancy rates (Wiemer et al, 1989), higher implantation ratio (Wetzels et al, 1998), and live births (Marcus and Brinsden, 1996). These effects are most pronounced with increasing the duration of co-culture (Wiemer et al, 1989), especially during the early cleavage stages which may be mediated by the expression of growth factors (Yeung et al, 1992).…”

mentioning

confidence: 99%

Effect of various co-culture systems on embryo development in ovine

Heidari¹,

Shirazi²,

Naderi³

et al. 2013

CZECH J ANIM SCI

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Considering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-free culture system. The IVM/IVF presumptive sheep zygotes were randomly cultured in different culture conditions as follows: (1) SOFaaBSA medium for the whole culture period (SOF, n = 371), (2) SOFaaBSA medium for the first 3 days followed by co-culturing with MSCs for the next 5 days (SOF-MSCs, n = 120), (3) co-culturing with MSCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (MSCs-SOF, n = 133), (4) co-culturing with MSCs for the whole culture period (MSCs, n = 212), (5) SOFaaBSA medium for the first 3 days followed by co-culturing with EFCs for the next 5 days (SOF-EFCs, n = 132), (6) co-culturing with EFCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (EFCs-SOF, n = 165), (7) co-culturing with EFCs for the whole culture period (EFCs, n = 236), and (8) co-culturing with OECs for the whole culture period (OECs, n = 255). One-Way ANOVA by multiple pairwise comparisons using Tukey's test was performed. Co-culturing in MSCs group had no superiority over EFCs and OECs groups. Though, when co-culturing with MSCs and EFCs was limited to the first 3 days of culture, the embryo development indices were improved compared to the other co-cultured groups. Considering both the hatching rate and total cell number, the application of MSCs for the first 3 days of culture (MSCs-SOF) was superior to the other co-culture and SOF groups.

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Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos

Cited by 40 publications

References 28 publications

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Effect of various co-culture systems on embryo development in ovine

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