Human Pancreatic Islets and Diabetes Research

Kaddis, John S.; Olack, Barbara; Sowinski, Janice; Cravens, James; Contreras, Juan L.; Niland, Joyce C.

doi:10.1001/jama.2009.482

Cited by 68 publications

(61 citation statements)

References 45 publications

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“…Human islets isolated at IIDP-affiliated isolation centers are shipped overnight to recipient laboratories (20). Both the number of investigators applying to receive human islets and the total number of requested islets have risen dramatically (21). Along with each islet preparation, the isolation center provides metrics of the isolation procedure, e.g., cold ischemic time, estimated culture time, and purity and viability of the islets, as well as deidentified donor information (age, Sex, BMI, race) that is released by the organ procurement organization.…”

mentioning

confidence: 99%

Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles

Kayton

Poffenberger

Henske

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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Powers AC. Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles. Am J Physiol Endocrinol Metab 308: E592-E602, 2015. First published February 3, 2015 doi:10.1152/ajpendo.00437.2014.-Human islet research is providing new insights into human islet biology and diabetes, using islets isolated at multiple US centers from donors with varying characteristics. This creates challenges for understanding, interpreting, and integrating research findings from the many laboratories that use these islets. In what is, to our knowledge, the first standardized assessment of human islet preparations from multiple isolation centers, we measured insulin secretion from 202 preparations isolated at 15 centers over 11 years and noted five distinct patterns of insulin secretion. Approximately three quarters were appropriately responsive to stimuli, but one quarter were dysfunctional, with unstable basal insulin secretion and/or an impairment in stimulated insulin secretion. Importantly, the patterns of insulin secretion by responsive human islet preparations (stable Baseline and Fold stimulation of insulin secretion) isolated at different centers were similar and improved slightly over the years studied. When all preparations studied were considered, basal and stimulated insulin secretion did not correlate with isolation center, biological differences of the islet donor, or differences in isolation, such as Cold Ischemia Time. Dysfunctional islet preparations could not be predicted from the information provided by the isolation center and had altered expression of genes encoding components of the glucose-sensing pathway, but not of insulin production or cell death. These results indicate that insulin secretion by most preparations from multiple centers is similar but that in vitro responsiveness of human islets cannot be predicted, necessitating preexperimental human islet assessment. These results should be considered when one is designing, interpreting, and integrating experiments using human islets.human; islet; function THE AVAILABILITY OF HUMAN ISLETS for basic and translational research has increased markedly over the past decade, fueling insights into human islet biology and diabetes. Studies of human islets have provided insight into human islet morphology, ␤-cell proliferation (2, 5, 6, 12), epigenetics (2, 4 -6, 12, 22

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mentioning

confidence: 99%

Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles

Kayton

Poffenberger

Henske

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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“…It is imperative that preoperative treatments, such as sildenafil, bosentan, and intravenous epoprostenol, be continued perioperatively. [66][67][68][69][70][71][72][73][74][75][76][77] Weaning after transplantation can take weeks. As additional therapy, inhaled nitric oxide (at doses of up to 80 ppm) can be delivered into the breathing circuit intraoperatively and postoperatively to reduce pulmonary artery pressures.…”

Section: Portopulmonary Hypertensionmentioning

confidence: 99%

Perioperative Care of the Liver Transplant Patient

Keegan

2016

Critical Care Clinics

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“…coh.org/) has created an automated matching system and an extremely efficient shipping mechanism to quickly ship viable human islets to multiple investigators. 9,10 More recently, the JDRF-supported nPOD program (http://www. jdrfnpod.org/) has made human pancreatic specimens (normal and diabetic) available to a growing number of investigators.…”

Section: Understanding Of Human Biology Has Greatly Increasedmentioning

confidence: 99%

Architecture and Morphology of Human Pancreatic Islets

Powers

2014

Cellular Endocrinology in Health and Disease

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Human Pancreatic Islets and Diabetes Research

Cited by 68 publications

References 45 publications

Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles

Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles

Perioperative Care of the Liver Transplant Patient

Architecture and Morphology of Human Pancreatic Islets

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