Human Papilloma Virus and Cancer Stem Cell markers in Oral Epithelial Dysplasia—An Immunohistochemical Study

Thankappan, Prasanth; Ramadoss, Madhavan Nirmal; Joseph, T Isaac; Augustine, Percy Ida; Shaga, I Bevin; Thilak, Jashree

doi:10.5041/rmmj.10451

Cited by 2 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was observed that the Ki-67 protein increased with advancing grades of OSCC which was also consistent with earlier research [23][24][25][26][27][28]. Nuclear Ki-67 positivity was identified in the peripheral region of tumor islands in well-differentiated OSCC.…”

Section: Discussionsupporting

confidence: 90%

“…This was in line with earlier research [ 23 , 24 ]. The rate of malignant transformation may be influenced by the degree of dysplasia, with high-grade dysplastic lesions being 4.5 times more likely to undergo such transformation than low-grade dysplastic lesions [ 23 - 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the final stages of mitosis, Ki-67 levels drop dramatically. Ki-67 has been found to play a crucial role in carcinogenesis, serving as a marker of tumor aggressiveness [23][24][25][26][27]. Furthermore, Ki-67 has also been implicated in tumor proliferation and invasion along with other markers like Bcl2 [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…Increased cell proliferation characterizes the shift of the normal oral epithelium to dysplasia and cancer. Thus, the Ki-67 protein may be used as a prognostic indicator for malignant transformation [26][27][28][29]. Hence, immunohistochemical expression of the cell cycleassociated protein Ki-67 was assessed in OED, OSCC, and NOM as a control in this study.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Ki-67 Expression as a Prognostic Marker: A Comparative Immunohistochemical Analysis of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Gupta¹,

Ramalingam²,

Yasothkumar³

et al. 2023

Cureus

View full text Add to dashboard Cite

Introduction Oral dysplasia is a frequent precancerous condition that may lead to oral cancer. The histopathologic abnormalities exhibited in a chronic, progressive, and premalignant condition of the oral mucosa are referred to as oral epithelial dysplasia (OED). It might show up as erythroplakia, leukoplakia, or leukoerythroplakia. OED is a premalignancy histologic marker that predicts a higher likelihood of squamous cell carcinoma development. Aims and objectives The aim of this study is to identify an association between Ki-67 protein expression and histological grading of OED and oral squamous cell carcinoma (OSCC) and to compare the expression of Ki-67 in different grades of OED and OSCC with the prognosis. Materials and methods The current retrospective research is focused on evaluating epithelial dysplasia and analyzing the function of Ki-67 as a prognostic marker after receiving institutional ethical approval. Group I - normal oral mucosa (NOM), Group II - OED, and Group III - OSCC were included in the study. For statistical analysis, SPSS Statistics version 21.0 (IBM Corp. Released 2021. IBM SPSS Statistics for Windows, Version 28.0. Armonk, NY: IBM Corp) was utilized. The Cox regression model was employed to look at interactions between various prognostic variables. At p<0.05, differences were deemed statistically significant. Results Ki-67 expression was confined to the basal layers in the normal oral epithelium and in the basal, suprabasal, and spinous layers in OED. Ki-67 positive cells were mostly found on the perimeter of well, moderate, and poorly differentiated OSCC tumor nests with Ki-67 positive cells scattered throughout OSCC. According to statistical analysis, there is a substantial difference in expression between OED and NOM, OSCC and NOM, and OED and OSCC. Conclusion Our study showed that there is a progressive increase in Ki-67 expression across various grades of OED, and the highest expression was noted in OSCC. Early identification and prompt treatment will help in improving the quality of life of such patients.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Ki-67 Expression as a Prognostic Marker: A Comparative Immunohistochemical Analysis of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Gupta¹,

Ramalingam²,

Yasothkumar³

et al. 2023

Cureus

View full text Add to dashboard Cite

show abstract

Analysis of the Correlation Between Papillomavirus Infection and the Expression of Tumor Suppressor Proteins in Patients With Oral Epithelial Dysplasia

Stepanov,

Hisamieva,

Tarasenko

et al. 2024

Actual Problems in Dentistry

View full text Add to dashboard Cite

Data from domestic and foreign literature indicate a close pathogenetic relationship between the expression of cancer markers p16 and p53, tumor suppressor proteins, and the invasion of human papillomavirus (HPV) in patients with precancerous lesions of the oral mucosa. Thus, it seems advisable to evaluate the frequency of detection of the expression of cancer markers p16 and p53 by immunohistochemical examination in patients with HPV-positive and HPV-negative dysplastic lesions of the oral mucosa. The aim is to increase the effectiveness of the diagnosis of lesions of the oral mucosa associated with epithelial dysplasia. Materials and methods. The study involved 50 patients with established diagnoses of leukoplakia and lichen planus with signs of epithelial dysplasia. After surgery, all patients underwent an immunohistochemical study of the expression of proteins p16 and p53 and a PCR study for papillomavirus. The ratio of the frequency of detection of cancer marker expression in subgroups depending on the HPV status was evaluated. Results. There were no statistically significant differences in the frequency of detection of p53 (p = 0.161) and p16 (p = 0.21) cancer marker expressions depending on the HPV status of patients. There were also statistically insignificant differences in the frequency of detection of the expression of cancer markers p16 (p = 0.333) and p53 (p = 0.178) depending on gender. The HPV-positive status of patients with epithelial dysplasia of the oral mucosa was statistically significantly more often associated with the female sex (p = 0.008). Conclusion. The assessment of the expression of proteins p16 and p53 is not a reliable method for diagnosing oral epithelial dysplasia and associated papillomavirus infection. There is a need to search for alternative and more accurate molecular markers of the disease, as well as a greater number of observations.

show abstract

Human Papilloma Virus and Cancer Stem Cell markers in Oral Epithelial Dysplasia—An Immunohistochemical Study

Cited by 2 publications

References 51 publications

Ki-67 Expression as a Prognostic Marker: A Comparative Immunohistochemical Analysis of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Ki-67 Expression as a Prognostic Marker: A Comparative Immunohistochemical Analysis of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Analysis of the Correlation Between Papillomavirus Infection and the Expression of Tumor Suppressor Proteins in Patients With Oral Epithelial Dysplasia

Contact Info

Product

Resources

About