Human papillomavirus and human telomerase RNA component gene in cervical cancer progression

Abstract

This study aimed to examine hTERC gene in different grades of cervical intraepithelial neoplasia (CIN) and cervical cancer, and the association between hTERC and high risk-human papillomavirus (HR-HPV) infection. Patients who underwent cervical cancer screening at the Second Affiliated Hospital of Kunming Medical University between October 2010 and December 2011 were enrolled. All patients underwent liquid-based cytology test and hybrid capture 2 (HC2) for HPV detection. hTERC was examined using fluorescence in situ hybridization (FISH). Cervical colposcopy biopsy was performed if any of the three results was positive. HC2, FISH, and pathology were compared. A total of 1200 women underwent screening, 150 patients underwent cervical biopsy: 32 in the normal group, 38 in the CIN1 group, 66 in the CIN2/3 group, and 14 in the invasive cervical cancer group. More patients had HR-HPV infection in the CIN2/3 group and ICC group compared with the CIN1 group. hTERC increased with increasing histological dysplasia. There was significant difference in hTERC positive rate between each of the three groups. More patients with hTERC gene amplification were observed in the positive HR-HPV group than in the HR-HPV negative group. In conclusion, hTERC is a potential marker for precancerous cervical cancer lesions. hTERC might be correlated with HR-HPV infection in cervical diseases.

The Role of p16/Ki-67 Immunostaining, hTERC Amplification and Fibronectin in Predicting Cervical Cancer Progression: A Systematic Review

“…Telomerase is a ribonucleoprotein enzyme complex that adds 50-TTAGGG-30 repeats to the chromosomal ends known as telomeres, which play an important part in maintaining chromosomal stability during DNA replication [ 21 , 23 , 24 ]. Human telomerase consists of three subunits: one RNA component (hTERC), which functions as a template for DNA replication; one of unknown function (TP1) and the human telomerase reverse transcriptase (hTERT) [ 95 , 96 ].…”

“…Moreover, the E6/E7 oncogene encoding the HPV proto-oncoprotein can up-regulate telomerase activity by human telomerase RNA component (hTERC) gene amplification. Studies have shown an important correlation between HR-HPV infection and hTERC up-regulation in CC progression [ 23 , 24 , 25 , 26 ]. Telomerase activity as a prognostic biomarker in CC has been demonstrated through numerous studies and it is generally recommended as an ancillary biomarker in CC screening, after cytology and HPV DNA detection.…”

Evaluation of anti-cancer potential of Excoecaria agallocha (L.) leaf extract on human cervical cancer (SiHa) cell line and assessing the underlying mechanism of action

“…At a global level, cervical cancer is one of the most common malignant forms of cancers among women [11]. In recent years, resistant to chemotherapeutics and increasing toxicity of the drugs have been observed that have decreased the efficiency of the existing treatment strategies for cervical cancer [12][13][14].…”

Bioinformatic Analyzes of the Association Between Upregulated Expression of JUN Gene via APOBEC-Induced FLG Gene Mutation and Prognosis of Cervical Cancer

“…According to the 2020 Global Cancer Statistics, CC ranks fourth in morbidity and mortality among women with cancer whereby there were 604,127 new cases and 41,831 deaths in the year ( 3 ). The highest global prevalence of CC occurs in developing countries (87%), of which it is the foremost form of gynecological cancer ( 4 ) whereas China accounts for 12% of the global incidence of CC and 11% of deaths ( 5 ). The CC screening program aims to reduce the incidence and mortality of the cancer ( 6 ).…”